Search results for "S-nitrosylation"
showing 10 items of 22 documents
Identification et analyse du rôle de protéines S‐nitrosylées lors de la modulation de la réponse immunitaire d’Arabidopsis thaliana par l’apo-pyoverd…
2019
Des travaux de l’UMR Agroécologie montrent que la réponse immunitaire des plantes est corrélée à leur nutrition en fer et est modulable par la pyoverdine, un sidérophore produit par la souche bactérienne bénéfique Pseudomonas fluorescens C7R12. Le traitement d’Arabidopsis thaliana exposée à une carence en fer par la pyoverdine dépourvue de fer (apo-pyo) restaure en effet la croissance de la plante aux dépens de son immunité. Nous avons émis l’hypothèse que le monoxyde d’azote (NO), une molécule signalétique ubiquitaire, est un acteur de la modulation de la réponse immunitaire des plantes exposées à l’apo-pyo et à sa souche productrice. Afin d’étayer cette hypothèse, trois axes seront dévelo…
Étude de la régulation des calmodulines de Nicotiana tabacum par le monoxyde d’azote
2014
Rapport de stage de Master II R SPE IPM; Master
Role Of S-Nitrosylation In The Extrinsic Apoptotic Signalling Pathway In Cancer.
2015
One of the key features of tumour cells is the acquisition of resistance to apoptosis. Thus, determining therapeutic strategies that circumvent apoptotic resistance and result in tumor regression is a challenge. One strategy to induce apoptosis is to activate death receptor signalling pathways. Members of the Tumor Necrosis Factor TNF-family death receptors ligand (TRAIL, FasL and TNF-α) can originate from immune and non-immune cells. Death receptors, engaged by cognate ligands, can initiate multiple signaling pathways, which can generate diverse outcomes, including non-apoptosis-related signal. Knowledge on the molecular mechanisms (that determine death or survival of tumour cells) followi…
Les protéines S-nitrosylées lors des réponses de défense des plantes. Cas de la protéine CDC48 et de l’EPSP Synthase
2012
Rapport de stage de Master 2 Recherche en Biochimie Biologie Cellulaire et Moléculaire (BBCM) SPE IPM CT non renseigné car non soutenu par INRA; Master
Emerging functions of nitric oxide in plant immunity
2016
SPEIPMUBAgrosupCNRS; The importance of nitric oxide (NO) in innate and adaptive immunity in mammals is well recognised. NO exerts antimicrobial properties against invaders but also displays immunoregulatory functions in which S-nitrosylation represents a signalling process of major importance. Over the last two decades, a growing body of evidence suggests that NO is also a major component of plant immunity. Our understanding of its role in plant defence has been enriched by the identification and functional analysis of S-nitrosylated proteins. The recent identification of new S-nitrosylated proteins including the chaperone-like enzyme cell division cycle 48 (CDC48), histone deacetylases (HD…
Nitric oxide (NO) in plants, a cell signalling messenger involved in plant defense. The case study of the N. tabacum / cryptogein model
2013
Communication vers les professionnels SPE IPM CT non renseigné car non soutenu par INRA
eNOS S-nitrosylates β-actin on Cys374 and regulates PKC-θ at the immune synapse by impairing actin binding to profilin-1.
2017
The actin cytoskeleton coordinates the organization of signaling microclusters at the immune synapse (IS); however, the mechanisms involved remain poorly understood. We show here that nitric oxide (NO) generated by endothelial nitric oxide synthase (eNOS) controls the coalescence of protein kinase C-¿ (PKC-¿) at the central supramolecular activation cluster (c-SMAC) of the IS. eNOS translocated with the Golgi to the IS and partially colocalized with F-actin around the c-SMAC. This resulted in reduced actin polymerization and centripetal retrograde flow of ß-actin and PKC-¿ from the lamellipodium-like distal (d)-SMAC, promoting PKC-¿ activation. Furthermore, eNOS-derived NO S-nitrosylated ß-…
NO Signalling in Plant Immunity
2016
The importance of nitric oxide (NO) in innate and adaptive immunity in mammals is well recognised. NO exerts antimicrobial properties against invaders but also displays immunoregulatory functions in which S-nitrosylation represents a signalling process of major importance. Over the last two decades, a growing body of evidence suggests that NO is also a major component of plant immunity. Our understanding of its role in plant defence has been enriched by the identification and functional analysis of S-nitrosylated proteins. The recent identification of new S-nitrosylated proteins including the chaperone-like enzyme cell division cycle 48 (CDC48), histone deacetylases (HDACs) and calmodulin (…
Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus
2017
Dysregulation of NO production is implicated in pregnancy-related diseases, including gestational diabetes mellitus (GDM). The role of NO and its placental targets in GDM pregnancies has yet to be determined. S-Nitrosylation is the NO-derived posttranslational protein modification that can modulate biological functions by forming NO-derived complexes with longer half-life, termed S-nitrosothiol (SNO). Our aim was to examine the presence of endogenous S-nitrosylated proteins in cysteine residues in relation to antioxidant defense, apoptosis, and cellular signal transduction in placental tissue from control (n=8) and GDM (n=8) pregnancies. S-Nitrosylation was measured using the biotin-switch …
Protein S-nitrosylation: What's going on in plants?
2012
International audience; Nitric oxide (NO) is now recognized as a key regulator of plant physiological processes. Understanding the mechanisms by which NO exerts its biological functions has been the subject of extensive research. Several components of the signaling pathways relaying NO effects in plants, including second messengers, protein kinases, phytohormones, and target genes, have been characterized. In addition, there is now compelling experimental evidence that NO partly operates through posttranslational modification of proteins, notably via S-nitrosylation and tyrosine nitration. Recently, proteome-wide scale analyses led to the identification of numerous protein candidates for S-…