Search results for "S5"
showing 10 items of 201 documents
Microvesicles shed by oligodendroglioma cells and rheumatoid synovial fibroblasts contain aggrecanase activity
2012
Membrane microvesicle shedding is an active process and occurs in viable cells with no signs of apoptosis or necrosis. We report here that microvesicles shed by oligodendroglioma cells contain an ‘aggrecanase’ activity, cleaving aggrecan at sites previously identified as targets for adamalysin metalloproteinases with disintegrin and thrombospondin domains (ADAMTSs). Degradation was inhibited by EDTA, the metalloproteinase inhibitor GM6001 and by tissue inhibitor of metalloproteinases (TIMP)-3, but not by TIMP-1 or TIMP-2. This inhibitor profile indicates that the shed microvesicles contain aggrecanolytic ADAMTS(s) or related TIMP-3-sensitive metalloproteinase(s). The oligodendroglioma cells…
En studie av de faktorer som bidrar til effekter ved PDAbruk i norsk hjemmesykepleie
2006
Masteroppgave i informasjonssystemer 2006 - Høgskolen i Agder, Kristiansand Helsesektoren i Norge er en belastet sektor som skal betjene et økende antall hjemmeboende klienter og bistå dem med helsetjenester og hjelp til daglige gjøremål. Etaten sliter med lav bemanning og begrensede midler. Dette har bidratt til at man har fått fremvekst av moderniseringstiltak i sektoren. Moderniseringen kan ta flere former og denne oppgaven ser i hovedsak på bruk av PDAer i norsk hjemmesykepleie. PDAer er små håndholdte PC-er som kan gi mobil tilgang til informasjon som tidligere var papirbasert og utilgjengelig for pleiepersonell ute på mobile oppdrag. Det er en rekke potensielle effekter ved slike PDA-…
Combined Therapy of Interferon Plus Ribavirin Promotes Multiple Adaptive Solutions in Hepatitis C Virus
2009
Hepatitis C virus (HCV) presents several regions involved potentially in evading antiviral treatment and host immune system. Two regions, known as PKR-BD and V3 domains, have been proposed to be involved in resistance to interferon. Additionally, hypervariable regions in the envelope E2 glycoprotein are also good candidates to participate in evasion from the immune system. In this study, we have used a cohort of 22 non-responder patients to combined therapy (interferon alpha-2a plus ribavirin) for which samples obtained just before initiation of therapy and after 6 or/and 12 months of treatment were available. A range of 25-100 clones per patient, genome region and time sample were obtained…
Leibniz, Modal Logic and Possible World Semantics: The Apulean Square as a Procrustean Bed for His Modal Metaphysics
2012
Even if Leibniz didn’t have the opportunity to actually conceive an explicit modal logic system, remains the fact that he had worked out a modal metaphysics, of which the inaugural act, in his Elementa juris naturalis (c. 1671) was an obvious reference to the Apulean square of opposition. Later, scholars acknowledged in this passage probably one of the first sketch of deontic logic of norms. His modal metaphysics rather deals with the so-called alethic modalities, sometimes expounded through a language such as R.M. Adams wondered whether Leibniz could be “a sort of grandfather of possible worlds semantics for modal logic”. In the following study, the Apulean square is used as a hermeneutic …
The effect of dentine pre-treatment using bioglass and/or polyacrylic acid on the interfacial characteristics of resin-modified glass ionomer cements.
2018
Objective To evaluate the effect of load-cycle aging and/or 6 months artificial saliva (AS) storage on bond durability and interfacial ultramorphology of resin-modified glass ionomer cement (RMGIC) applied to dentine air-abraded using Bioglass 45S5 (BAG) with/without polyacrylic acid (PAA) conditioning.Methods RMGIC (Ionolux, VOCO) was applied onto human dentine specimens prepared with silicon-carbide abrasive paper or air-abraded with BAG with or without the use of PAA conditioning. Half of bonded-teeth were submitted to load cycling (150,000 cycles) and half immersed in deionised water for 24 h. They were cut into matchsticks and submitted immediately to microtensile bond strength (μTBS) …
Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation
1995
Processing of the hepatitis C virus polyprotein is mediated by host cell signalases and at least two virally encoded proteinases. Of these, the serine-type proteinase encompassing the amino-terminal one-third of NS3 is responsible for cleavage at the four sites carboxy terminal of NS3. The activity of this proteinase is modulated by NS4A, a 54-amino-acid polyprotein cleavage product essential for processing at the NS3/4A, NS4A/4B, and NS4B/5A sites and enhancing cleavage efficiency between NS5A and NS5B. Using the vaccinia virus-T7 hybrid system to express hepatitis C virus polypeptides in BHK-21 cells, we studied the role of NS4A in proteinase activation. We found that the NS3 proteinase a…
Resistance test guided retreatment of HCV infected patients with a previous failure to a NS5A inhibitor-containing regimen: the Italian Vironet C rea…
2019
Previous article in issueNext article in issue Introduction: There is a limited documentation about the retreatment of patients failing a recommended NS5A-containing regimen in Italy. Materials & methods: Within the VIRONET-C network, 386 NS5A-failing patients infected with different HCV-genotypes (GT) (GT1a/1b/2a-c/3a-b-g-h/4a-d-n-o-v=93/124/19/112/38) were analyzed. Retreatment of 105 failures was investigated. HCV-resistance-test was performed by Sanger-sequencing. Results: Failures following seven different NS5A-containing regimens were studied: 3D/2D (paritaprevir/ombitasvir ± dasabuvir) ± ribavirin (N = 72/4), daclatasvir/ledipasvir/velpatasvir + sofosbuvir ± ribavirin (N = 105/13…
Galvanic Deposition of Calcium Phosphate/Bioglass Composite Coating on AISI 316L
2023
Calcium phosphate/Bioglass composite coatings on AISI 316L were investigated with regard to their potential role as a beneficial coating for orthopedic implants. These coatings were realized by the galvanic co-deposition of calcium phosphate compounds and Bioglass particles. A different amount of Bioglass 45S5 was used to study its effect on the performance of the composite coatings. The morphology and chemical composition of the coatings were investigated before and after their aging in simulated body fluid. The coatings uniformly covered the AISI 316L substrate and consisted of a brushite and hydroxyapatite mixture. Both phases were detected using X-ray diffraction and Raman spectroscopy.…
Transitioning towards a Sustainable Wellbeing Economy—Implications for Rural–Urban Relations
2021
This article focuses on the question of how a shift from a narrow economic perspective to a wider sustainable wellbeing focus in regional development strategies and actions might change rural–urban relations. A brief review of relevant research and discourses about economic development models provides the foundation for the analysis. The review leads to the development of an analytical framework that puts the notion of sustainable wellbeing at its center. The criteria included in the analytical framework are then used to assess the current situation, challenges and perceived ways forward based on data and analyses from 11 European regions. The focus of the analysis is on different exp…
Variabilidad clonal de la polimerasa NS5b del virus de la hepatitis C en aislados de muestras clínicas
2010
La infección crónica provocada por el virus de la hepatitis C (VHC) sigue siendo un gran problema de salud pública a nivel mundial. Se estima que 200 millones de personas (3% de la población mundial) se encuentran infectadas por este virus.El tratamiento actual basado en la combinación de dos fármacos no específicos, el interferón-α pegilado y la ribavirina; provoca numerosos efectos secundarios, el tratamiento es de larga duración y además es costoso y tiene poco éxito en pacientes infectados con genotipo 1. Esto ha impulsado al desarrollo de nuevos fármacos más específicos. Los más prometedores son los dirigidos contra proteínas específicas del virus, como la proteasa NS3 o la RNA polimer…