Search results for "SAC"

showing 10 items of 3337 documents

Purification of a glucose-binding protein from rat liver nuclei. Evidence for a role in targeting of nuclear mRNP to nuclear pore complex.

1992

A nuclear carbohydrate-binding protein with a molecular mass of 67 kDa (CBP67), which is specific for glucose residues, was purified to essential homogeneity from rat liver nuclear extracts. This protein could also be isolated from nuclear ribonucleoprotein (RNP) complexes by extraction in the presence of 0.6 M or 2 M NaCl, but it was absent in polysomal RNP complex. The binding of the purified protein, which has an isoelectric point of 7.3, to glucose-containing glycoconjugates depends on the presence of Ca2+ and Mg2+. Using closed nuclear envelope vesicles as a system to study nuclear transport of RNA, it was shown that both entrapped polysomal mRNA and nuclear RNA precursors are readily …

MaleMonosaccharide Transport ProteinsCations DivalentBiologyBiochemistryAnimalsHumansMagnesiumRNA MessengerNuclear proteinNuclear poreCell NucleusBinding proteinNuclear cap-binding protein complexBiological TransportRats Inbred StrainsRatsMessenger RNPGlucose bindingMolecular WeightBiochemistryLiverRibonucleoproteinsCalciumElectrophoresis Polyacrylamide GelNucleoporinNuclear transportIsoelectric FocusingHeLa CellsEuropean journal of biochemistry
researchProduct

Subcellular Localization of GLUT4 in Nonstimulated and Insulin-Stimulated Soleus Muscle of Rat

1992

Soleus muscles of fed rats were fixed by vascular perfusion with paraformaldehyde; individual fibers were teased and immunostained with a polyclonal antibody against the COOH-terminal of GLUT4. The binding sites were visualized by a horseradish peroxidase–coupled secondary antibody and diaminobenzidine. The fibers were embedded in epoxy resin and studied by electron microscopy. Strong immunoreactivity was found in subsarcolemmal clusters of vesicles and cisternae, Golgilike structures, and triadic junctions. Clusters of vesicles between myofibrils were occasionally stained. The plasma membrane was unlabeled. However, the plasma membrane was labeled when the rats had been injected with insul…

MaleMonosaccharide Transport ProteinsEndocrinology Diabetes and MetabolismGlucose uptakeCoated vesicleImmunoenzyme TechniquesCell membraneSarcolemmaInternal MedicinemedicineAnimalsInsulinSarcolemmabiologyMusclesVesicleCell MembraneRats Inbred StrainsRatsmedicine.anatomical_structureBiochemistrybiology.proteinBiophysicsMyofibrilGLUT4IntracellularDiabetes
researchProduct

Suppression of leukotriene B4 and tumour necrosis factor alpha release in acute inflammatory responses by novel prenylated hydroquinone derivatives.

1998

A series of prenyl hydroquinone derivatives synthesized as structural analogs of marine products were tested for their effects on inflammatory responses in vitro and in vivo. 2-Prenyl-1,4-hydroquinone (H1), 2-diprenyl-1,4-hydroquinone (H2), 2-triprenyl-1,4-hydroquinone (H3) and 2-tetraprenyl-1,4-hydroquinone (H4) scavenged reactive oxygen species and inhibited 5-lipoxygenase (5-LO) activity in human neutrophils. The inhibition of 5-LO activity was demonstrated in vivo in the mouse air pouch injected with zymosan and arachidonic acid-induced ear inflammation. The four compounds suppressed the production of tumour necrosis factor alpha (TNFalpha) in J774 cells stimulated with lipopolysacchari…

MaleNecrosisLipopolysaccharideLeukotriene B4Anti-Inflammatory AgentsPharmacologyLeukotriene B4Dinoprostonechemistry.chemical_compoundMiceIn vivomedicineAnimalsEdemaHumansCells CulturedNitritesPharmacologyInflammationArachidonic AcidbiologyTumor Necrosis Factor-alphaZymosanGeneral MedicineHydroquinonesNitric oxide synthasechemistryBiochemistryDepression ChemicalArachidonate 5-lipoxygenaseLuminescent Measurementsbiology.proteinTumor necrosis factor alphamedicine.symptomNaunyn-Schmiedeberg's archives of pharmacology
researchProduct

Associations between Adolescents’ Interpersonal Relationships, School Well-being, and Academic Achievement during Educational Transitions

2020

AbstractA youth’s ability to adapt during educational transitions has long-term, positive impacts on their academic achievement and mental health. Although supportive relationships with parents, peers, and teachers are protective factors associated with successful educational transitions, little is known about the reciprocal link between the quality of these interpersonal relationships and school well-being, with even less known about how these two constructs affect academic achievement. This longitudinal study examined how the quality of interpersonal relationships and school well-being worked together to affect academic achievement during the transition from primary school to lower second…

MaleParentsearly adolescenceLongitudinal studyinterpersonal relationshipsInterpersonal relationshipsvarhaisnuoretAcademic achievementEmpirical ResearchAcademic achievementihmissuhteetalakouluDevelopmental psychologySchool well-beinghenkinen hyvinvointinuoretTransactional leadershipeducational transitionDevelopmental and Educational PsychologyLongitudinal StudiesParent-Child RelationsChildAcademic SuccessSchoolsParenting4. Education05 social sciences050301 educationacademic achievementHealth psychologyFemaleyläkouluPsychology050104 developmental & child psychologyopintomenestysAdolescentSocial PsychologyeducationEducational transitionAffect (psychology)Peer GroupEducationInterpersonal relationshipHumansInterpersonal Relations0501 psychology and cognitive sciencesEarly adolescenceAchievementschool well-beingMental healthPersonality DevelopmentWell-being516 Educational sciences0503 educationSocial Sciences (miscellaneous)Journal of Youth and Adolescence
researchProduct

Clinicopathological evaluation of 164 dental follicles and dentigerous cysts with emphasis on the presence of odontogenic epithelium in the connectiv…

2012

Objectives: Some ameloblastomas presumably originate from odontogenic epithelium within the connective tissue of dental follicles and dentigerous cysts. Therefore, it would seem reasonable to discuss as whether odontogenic epithelium proliferations, frankly displaying ameloblastomatous features (“focal ameloblastoma”), should be considered as an “early” ameloblastoma. Study Design: Histopathological reports from 164 dental follicles and dentigerous cysts from the Department of Oral and Maxillofacial Surgery/Oral Pathology of the VU Free University medical center in Amsterdam, The Ne-therlands, were reviewed. Histopathological slides from 39 cases reporting the presence of odontogenic epithe…

MalePathologymedicine.medical_specialtyDentigerous CystConnective tissueOdontologíaEpitheliumAmeloblastomaYoung AdultSDG 3 - Good Health and Well-beingDental SacOral and maxillofacial pathologymedicineHumansAmeloblastomaGeneral DentistryDental folliclebusiness.industryDental Sac:CIENCIAS MÉDICAS [UNESCO]medicine.diseaseCiencias de la saludEpitheliumDentigerous cystmedicine.anatomical_structureOtorhinolaryngologyConnective TissueUNESCO::CIENCIAS MÉDICASOral and maxillofacial surgery/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_beingFemaleResearch-ArticleSurgeryOral Surgerybusiness
researchProduct

Performance of a Predictive Model for Long-Term Hemoglobin Response to Darbepoetin and Iron Administration in a Large Cohort of Hemodialysis Patients

2016

International audience; Anemia management, based on erythropoiesis stimulating agents (ESA) and iron supplementation, has become an increasingly challenging problem in hemodialysis patients. Maintaining hemodialysis patients within narrow hemoglobin targets, preventing cycling outside target, and reducing ESA dosing to prevent adverse outcomes requires considerable attention from caregivers. Anticipation of the long-term response (i.e. at 3 months) to the ESA/iron therapy would be of fundamental importance for planning a successful treatment strategy. To this end, we developed a predictive model designed to support decision-making regarding anemia management in hemodialysis (HD) patients tr…

MalePediatricsBlood transfusionDarbepoetin alfaPhysiologymedicine.medical_treatment030232 urology & nephrologylcsh:Medicine030204 cardiovascular system & hematologyFerric CompoundsBiochemistryGlucaric AcidHemoglobinsMathematical and Statistical Techniques0302 clinical medicineMedicine and Health SciencesDarbepoetin alfaErythropoiesislcsh:ScienceFerric Oxide SaccharatedMultidisciplinaryPharmaceuticsDisease ManagementAnemia[SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/HematologyHematologyMiddle Aged3. Good healthNephrologyInjections IntravenousPhysical SciencesFemaleHemodialysisStatistics (Mathematics)Research Articlemedicine.drugComputer and Information Sciencesmedicine.medical_specialtyAnemiaResearch and Analysis Methods03 medical and health sciencesDose Prediction MethodsRenal DialysisArtificial IntelligenceMedical DialysismedicineHumansHemoglobinDosingStatistical MethodsIron Deficiency AnemiaIntensive care medicineArtificial Neural NetworksAgedRetrospective StudiesComputational NeuroscienceModels Statisticalbusiness.industrylcsh:RBiology and Life SciencesComputational BiologyProteinsRetrospective cohort studymedicine.diseaseIron-deficiency anemiaHematinicsKidney Failure ChronicCognitive Sciencelcsh:QNeural Networks ComputerHemoglobinPhysiological ProcessesbusinessMathematicsNeuroscienceForecasting
researchProduct

Importance of surgical history in diagnosing mucopolysaccharidosis type II (Hunter syndrome): data from the Hunter Outcome Survey.

2010

Purpose: To characterize surgical histories typical of patients with mucopolysaccharidosis type II, thereby broadening understanding of the natural history of these patients and helping physicians recognize the disease. Methods: Data on surgical interventions from the Hunter Outcome Survey—a multinational, observational database of patients with mucopolysaccharidosis type II—were analyzed. The study population comprised 527 patients for whom surgical data were reported on/before July 23, 2009. Results: Surgical interventions were performed in 83.7% of the study population. Patients underwent their first operation at a median age of 2.6 years. Tympanostomies, repairs of inguinal hernias, and…

MalePediatricsmedicine.medical_specialtyAdolescentMucopolysaccharidosisPopulationYoung AdultAge DistributionmedicineHumansHerniaMucopolysaccharidosis type IIChildeducationCarpal tunnel syndromeGenetics (clinical)Mucopolysaccharidosis IIeducation.field_of_studybusiness.industryData CollectionInfantHunter syndromemedicine.diseaseSurgeryNatural historyTreatment OutcomeChild PreschoolSurgical Procedures OperativeHunter syndromePopulation studySettore MED/35 - MALATTIE CUTANEE E VENEREEbusiness
researchProduct

A dose-range study assessing immunogenicity and safety of one dose of a new candidate meningococcal serogroups A, C, W-135, Y tetanus toxoid conjugat…

2009

Meningococcal disease incidence is highest in young children, yet a tetravalent conjugate vaccine is currently not available for this age group. This study evaluated a single dose of four different ACWY-TT conjugate vaccine formulations in 240 toddlers (12-14 months) and 268 children (3-5 years) compared to licensed age-appropriate control vaccines. In toddlers, rSBA-MenC GMTs for the selected formulation were statistically higher than after monovalent-MenC-conjugate vaccine. In children, rSBA-GMTs against each serogroup were statistically higher than after tetravalent polysaccharide vaccine. The safety profile was comparable to licensed controls. The new ACWY-TT conjugate vaccine promises …

MalePediatricsmedicine.medical_specialtyMeningococcal VaccinesMeningococcal diseasePolysaccharide Vaccinemedicine.disease_causeConjugate vaccinemedicineTetanus ToxoidHumansVaccines ConjugateGeneral VeterinaryGeneral Immunology and MicrobiologyTetanusbusiness.industryImmunogenicityNeisseria meningitidisPublic Health Environmental and Occupational HealthToxoidInfantmedicine.diseaseAntibodies BacterialMeningococcal InfectionsInfectious DiseasesChild PreschoolImmunologyMolecular MedicineFemalebusinessConjugateVaccine
researchProduct

Circulating neprilysin hypothesis: A new opportunity for sacubitril/valsartan in patients with heart failure and preserved ejection fraction?

2021

BackgroundCirculating Neprilysin (sNEP) has emerged as a potential prognostic biomarker in heart failure (HF). In PARAGON-HF benefit of sacubitril/valsartan was only observed in patients with left ventricular ejection fraction (LVEF) ≤57%. We aimed to assess the prognostic value of sNEP in outpatients with HF and LVEF >57%, in comparison with patients with LVEF ≤57%.MethodsConsecutive HF outpatients were included from May-2006 to February-2016. The primary endpoint was the composite of all-cause death or HF hospitalization and the main secondary endpoint was the composite of cardiovascular death or HF hospitalization. For the later competing risk methods were used.ResultssNEP was measure…

MalePhysiologyCancer TreatmentCardiovascular MedicineBiochemistrySacubitrilMedical ConditionsClinical endpointMedicine and Health SciencesMultidisciplinaryEjection fractionPharmaceuticsAminobutyratesHazard ratioQRMiddle AgedPrognosisBody FluidsCardiovascular TherapyBloodValsartanOncologyCardiovascular DiseasesCardiologycardiovascular systemMedicineValsartanFemaleNeprilysinAnatomymedicine.drugcirculatory and respiratory physiologyResearch Articlemedicine.medical_specialtyScienceCardiologyDrug TherapyInternal medicinemedicineHumanscardiovascular diseasesAgedProportional Hazards ModelsHeart FailureProportional hazards modelbusiness.industryBiphenyl CompoundsBiology and Life SciencesStroke VolumeCardiovascular Disease Riskmedicine.diseaseHeart failurebusinessSacubitril ValsartanBiomarkersReceptor Antagonist TherapyEjection FractionPloS one
researchProduct

Serum hexosaminidase and ß-glucuronidase activities in infants: effects of age and sex

2003

We investigated the effect of age and sex on the serum activity of hexosaminidase (HEX) and ß-glucuronidase (BGLU) in 275 normal term infants aged 12 h to 12 months. Up to six weeks of life, HEX was significantly higher in boys (P<=0.023). During the age period of 1-26 weeks, BGLU was also higher in boys, but differences were significant only at 2-6 and 7-15 weeks (P<=0.016). The developmental pattern of HEX and BGLU was sex dependent. HEX activity increased in both sexes from 4-7 days of life, reaching a maximum of 1.4-fold the birth value at 2-6 weeks of age in boys (P<0.001) and a maximum of 1.6-fold at 7-15 weeks in girls (P<0.001). HEX activity gradually decreased thereafter, reaching …

MalePhysiologyImmunologyBiophysicsPhysiologyLysosomal storage diseaseFirst year of lifeAge and sexBiochemistrySex FactorsGangliosidoses GM2GM2 gangliosidosisHumansMedicineHexosaminidaseGeneral Pharmacology Toxicology and PharmaceuticsMucopolysaccharidosis type VIIlcsh:QH301-705.5GlucuronidaseAnalysis of Variancelcsh:R5-920business.industryGeneral NeuroscienceAge FactorsInfant NewbornMucopolysaccharidosis VIIInfantHexosaminidaseCell BiologyGeneral Medicinebeta-N-Acetylhexosaminidaseslcsh:Biology (General)Femalelcsh:Medicine (General)businessS glucuronidaseß-GlucuronidaseBiomarkersBrazilian Journal of Medical and Biological Research
researchProduct