Search results for "SAC"

Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats

2020

Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes. We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice. Here, we aimed at targeting F. prausnitzii by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD. First, the effect of XOS on F. prausnitzii growth was assessed in vitro. Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group). XOS increased F. prausnitzii growth, having onl…

The anaerobic metabolism of malate of Saccharomyces bailii and the partial purification and characterization of malic enzyme.

1982

1. The main pathway of the anaerobic metabolism of l-malate in Saccharomyces bailii is catalyzed by a l-malic enzyme. 2. The enzyme was purified more than 300-fold. During the purification procedure fumarase and pyruvate decarboxylase were removed completely, and malate dehydrogenase and oxalacetate decarboxylase were removed to a very large extent. 3. Manganese ions are not required for the reaction of malic enzyme of Saccharomyces bailii, but the activity of the enzyme is increased by manganese. 4. The reaction of l-malic enzyme proceeds with the coenzymes NAD and (to a lesser extent) NADP. 5. The Km-values of the malic enzyme of Saccharomyces bailii were 10 mM for l-malate and 0.1 mM for…

FIRST STAGES OF MICROSPORE REPROGRAMMING TO EMBRYOGENESIS THROUGH ISOLATED MICROSPORE CULTURE IN LOQUAT

2011

The current experiments were undertaken to develop a method for regenerating doubled haploid (DH) plants of Eriobotrya japonica (Thunb.), Rosaceae, subfamily Maloideae through isolated microspore culture of nine loquat cultivars. Protocols were developed for isolation and culture of loquat microspores. Isolated microspores in vitro cultured started dividing and developing multicellular, globular and irregular structures. After 4 weeks of culture, the responding microspores produced yellowish/white callus. The embryogenic response of microspores was highly dependent on the cultivar and on the medium composition.

Apple (Malus domestica) and Tomato (Lycopersicum esculentum) Fruits Cell-Wall Hemicelluloses and Xyloglucan Degradation during Penicillium expansum I…

2004

We characterized the changes in cell-wall hemicellulosic polysaccharides and the hemicellulose-degrading enzymes associated with apple and tomato fruits infected by Penicillium expansum. Our results showed a reduction in the molecular mass of hemicelluloses, with this reduction being particularly notable in the xyloglucan associated with P. expansum infection. The activation of fungal beta-glucanases was also highlighted. Fruit xyloglucan endotransglucosylase/hydrolase (XTH)-specific activity decreased drastically during the infection process in both apple and tomato fruits. We suggest that XTH reduction during the infection might be related with the fungus attack mechanism. We also suggest…

Yeast Chronological Lifespan: Longevity Regulatory Genes and Mechanisms

2022

S. cerevisiae plays a pivotal role as a model system in understanding the biochemistry and molecular biology of mammals including humans. A considerable portion of our knowledge on the genes and pathways involved in cellular growth, resistance to toxic agents, and death has in fact been generated using this model organism. The yeast chronological lifespan (CLS) is a paradigm to study age-dependent damage and longevity. In combination with powerful genetic screening and high throughput technologies, the CLS has allowed the identification of longevity genes and pathways but has also introduced a unicellular “test tube” model system to identify and study macromolecular and cellular damage lead…

The Aesthetics of Healing in the Sacredness of the African American Female’s Bible: Zora Neale Hurston’s Moses, Man of the Mountain

2016

Zora Neale Hurston’s Moses, Man of the Mountain (1939) stands in the tradition of African American use of the biblical musings that aims to relativize and yet uphold a new version of the sacred story under the gaze of a black woman that manipulates and admonishes the characters of the gospel to offer a feminist side of the Bible. The novel discloses Hurston’s mastering of the aesthetics that black folklore infused to the African American cultural experience and her accommodation to bring to the fore the needed voice of black women. Rejecting the role of religion as a reductive mode of social protest, the novel extends its jeremiadic ethos and evolves into a black feminist manifesto in which…

FROM Δηομένη TO Madonna dell’Itria... AND VICEVERSA. TWO MARIAN ICONOGRAPHIES SUPERIMPOSED IN A “PALIMPSEST WALL” OF MARSALA (TP)

2019

During a recent restoration in an underground chapel in Marsala (TP), the wall to which the altar is attached proved to be a palimpsest of several layers. The oldest dated back from the late twelfth to early thirteenth century displaying a “velarium” in the lower register. Overhead we see a Madonna in prayer (Δηομένη) preceded by a theory of Saints. A second layer, from the 15th century, decisively changes the original iconography thus transforming it into the typically Sicilian iconography of the “Madonna dell’Itria”. The Child, positioned in the center of the Mother’s breast, sits inside a chest supported by two old chants. The latter devotional image, due to the perishability of the plas…

The Shwartzman reaction repealed

2007

The article, “ -Galactosylceramide induces protection against lipopolysaccharide-induced shock” (doi:10.1189/jlb.0506298), was selected as a Pivotal Advance because the results suggest that -galactosylceramide ( GalCer), a glycolipid isolated from marine sponges, can protect against the complete morbidity and mortality characterisitic of endotoxin shock by inducing NKT cells to produce TH2 cytokines.

2019

Pre-industrial money supply typically consisted of multiple, often foreign currencies. Standard economic theory implies that this entails welfare loss due to transaction costs imposed by currency exchange. Through a study of novel data on Finnish nineteenth-century parish-level currency conditions, we show that individual currencies had principal areas of circulation, with extensive co-circulation restricted to the boundary regions in between. We show that trade networks, defined here through the regional co-movement of grain prices, proved crucial in determining the currency used. Market institutions and standard price mechanisms had an apparent role in the spread of different currencies a…

Transaction management for m-commerce at a mobile terminal

2006

Although there has been a lot of discussion of "transactions" in mobile e-commerce (m-commerce), very little attention has been paid for distributed transactional properties of the computations facilitating m-commerce. In this paper, we first present a requirement analysis and then present a wireless terminal-based transaction manager (TM) architecture. This architecture is based on the assumption that there is an application that supports certain business transaction(s) and that it uses the TM to store transactional state information and retrieve it after a communication link, application, or terminal crash. We present the design of such a TM, including the application interface, modules a…