Search results for "SAMPLE"
showing 10 items of 2270 documents
Stroke Analysis in Padel According to Match Outcome and Game Side on Court
2020
The aim of this study was to analyze the distribution of padel strokes, their effectiveness, direction, and court zone, comparing between the winning and losing pairs in the match and the playing side of the players. The sample included 8441 strokes corresponding to 1055 points out of a total of nine padel matches in the First National Category. The variables analyzed were type of stroke, court area, effectiveness and directions of the strokes, match outcome, and game side. Matches were analyzed through systematic observation. The results showed that the winning pair made a significantly higher percentage of winners, and cross-court smashes and volleys from the offensive zone. In addition, …
Pilot study on university students' opinion about STEM studies at higher education
2020
The percentages of women enrolled in higher education in the STEM sector are significantly lower than those of men. Overall, gender representation in science, technology, engineering and mathematics degrees in Europe is not balanced. The Leaky Pipeline phenomenon, marked by gender stereotypes, makes the latent gender gap a relevant topic of study. Studies exist on academic performance, self-perception, self-efficacy, outcome expectations; however, studying gender stereotypes linked to STEM studies is also essential. It is necessary to know the social and family context in which young people have grown up, as well as their perception of such studies. To study gender stereotypes of university…
The all-on-four treatment concept: Systematic review
2017
Objectives To systematically review the literature on the "all-on-four" treatment concept regarding its indications, surgical procedures, prosthetic protocols and technical and biological complications after at least three years in function. Study design The three major electronic databases were screened: MEDLINE (via PubMed), EMBASE, and the Cochrane Library of the Cochrane Collaboration (CENTRAL). In addition, electronic screening was made of the 'grey literature' using the System for Information on Grey Literature in Europe - Open Grey, covering the period from January 2005 up to and including April 2016. Results A total of 728 articles were obtained from the initial screening process. O…
Evaluation of FASP, SP3, and iST Protocols for Proteomic Sample Preparation in the Low Microgram Range
2017
Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Here, we performed an independent comparison between single-pot solid-phase-enhanced sample preparation (SP3), filter-aided sample preparation (FASP), and a commercial kit based on the in-StageTip (iST) method. We assessed their performance for the processing of proteomic samples in the low μg range using varying amounts of HeLa cell lysate (1-20 μg of total protein). All three workflows showed similar performances for 20 μg of starting material. When handling sample sizes below 10 μg, the number of identified proteins and peptides as well as the quantitat…
Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
2019
The use of isolated ocular blood vessels in vitro to decipher the pathophysiological state of the eye using advanced technological approaches has greatly expanded our understanding of certain diseases. Mass spectrometry (MS)-based proteomics has emerged as a powerful tool to unravel alterations in the molecular mechanisms and protein signaling pathways in the vascular beds in health and disease. However, sample preparation steps prior to MS analyses are crucial to obtain reproducible results and in-depth elucidation of the complex proteome. This is particularly important for preparation of ocular microvessels, where the amount of sample available for analyses is often limited and thus, pose…
Proteomics of CaCO3 biomineral-associated proteins: how to properly address their analysis.
2013
8 pages; International audience; In a recent editorial (Proc. Natl. Acad. Sci., 2013 110, E2144-E2146) and elsewhere, questions have been raised regarding the experimental practices in relation to the proteomic analysis of organic matrices associated to the biomineralized CaCO3 skeletons of metazoans such as molluscan shells and coral skeletons. Indeed, although the use of new high sensitivity MS technology potentially allows to identify a greater number of proteins, it is also equally (or even more) sensitive to contamination of residual proteins from soft tissues, which are in close contact with the biomineral. Based on our own past and present experimental know-how-observations that are …
Comparison of classification methods that combine clinical data and high-dimensional mass spectrometry data
2013
Background The identification of new diagnostic or prognostic biomarkers is one of the main aims of clinical cancer research. Technologies like mass spectrometry are commonly being used in proteomic research. Mass spectrometry signals show the proteomic profiles of the individuals under study at a given time. These profiles correspond to the recording of a large number of proteins, much larger than the number of individuals. These variables come in addition to or to complete classical clinical variables. The objective of this study is to evaluate and compare the predictive ability of new and existing models combining mass spectrometry data and classical clinical variables. This study was co…
Recent Advances in Affinity MOF-Based Sorbents with Sample Preparation Purposes
2020
This review summarizes the recent advances concerning metal–organic frameworks (MOFs) modified with several biomolecules (e.g., amino acids, nucleobases, proteins, antibodies, aptamers, etc.) as ligands to prepare affinity-based sorbents for application in the sample preparation field. The preparation and incorporation strategies of these MOF-based affinity materials were described. Additionally, the different types of ligands that can be employed for the synthesis of these biocomposites and their application as sorbents for the selective extraction of molecules and clean-up of complex real samples is reported. The most important features of the developed biocomposites will be discussed thr…
Spanish human proteome project: Dissection of chromosome 16
2013
11 páginas, 6 figuras.-- et al.
Feasibility of shotgun urinary proteomics for investigating prematurely born preschoolers (PBP)
2016
Background: Preterms and twins are at higher risk of respiratory morbidity later in life. Advances in proteomic approaches may allow the characterization of biomarkers involved in respiratory diseases (Mauri et al. Imm. Lett. 2014;162:2-10). Gel-free approach quantitatively identify differentially expressed proteins in relation to physiopathological conditions (Mauri&Dehò, Meth Enzymology 2008;447:99-117). This can improve the clinical reliability of the next generation of biomarkers to discriminate multiple phenotypes of childhood respiratory diseases. Aim: To assess the ability of gel-free proteomics for identifying specific protein profiles related to PBP. Methods: Urine samples were…