Search results for "SAMPLE"
showing 10 items of 2270 documents
Motives for buying local, organic food through English box schemes
2018
Purpose The purpose of this paper is to explain the growing interest of English consumers in local organic food sold through box schemes, by providing insights into the motives of customers of such schemes and examining the relationship with their awareness about problems of the agro-food system. Design/methodology/approach A mixed methods approach combined in-depth interviews with 22 box scheme customers with a quantitative survey of 416 consumers, analysed by means of principal component analysis and an ordered logit model. Findings Consumers of small local organic box schemes in England are both altruistically and hedonistically motivated. This includes a strong political motivation to …
Systematic review of guidelines for internal validity in the design, conduct and analysis of preclinical biomedical experiments involving laboratory …
2020
Over the last two decades, awareness of the negative repercussions of flaws in the planning, conduct and reporting of preclinical research involving experimental animals has been growing. Several initiatives have set out to increase transparency and internal validity of preclinical studies, mostly publishing expert consensus and experience. While many of the points raised in these various guidelines are identical or similar, they differ in detail and rigour. Most of them focus on reporting, only few of them cover the planning and conduct of studies. The aim of this systematic review is to identify existing experimental design, conduct, analysis and reporting guidelines relating to preclinic…
Improving efficiency of viability-qPCR for selective detection of infectious HAV in food and water samples.
2017
Aim: To improve the efficacy of intercalating dyes to distinguishing between infectious and inactivated hepatitis A virus (HAV) in food. Methods and Results: Different intercalating dyes were evaluated for the discrimination between infectious and thermally inactivated HAV suspensions combining with the RT‐qPCR proposed in the ISO 15216. Among them, PMAxx was the best dye in removing the RT‐qPCR signal from inactivated HAV. Applied to lettuce and spinach, PMAxx–Triton pretreatment resulted in complete removal of the RT‐qPCR signal from inactivated HAV. Likewise, this study demonstrates that this pretreatment is suitable for the discrimination of inactivated HAV in shellfish without further …
A Method for Isolation of the Virome from Plasma Samples
2018
Virome studies are of special interest nowadays. Understanding viral communities in different body compartments will help guide future personalized treatments and to discern between homeostasis and disease. High-throughput sequencing technologies allow us to detect all the nucleic acids present in a sample, including viral ones, by random sequencing. One of the major challenges in virome studies is the correct isolation of the viral nucleic acids from a specific sample. This can be done during the extraction steps (e.g., enrichment of viral capsids), or during the bioinformatic analysis (e.g., removing all human and bacterial sequences). Furthermore, it is an important remark that the treat…
Label-Free Proteomics of Quantity-Limited Samples Using Ion Mobility-Assisted Data-Independent Acquisition Mass Spectrometry
2021
Over the past two decades, unbiased data-independent acquisition (DIA) approaches have gained increasing popularity in the bottom-up proteomics field. Here, we describe an ion mobility separation enhanced DIA workflow for large-scale label-free quantitative proteomics studies where starting material is limited. We set a special focus on the single pot solid-phase-enhanced sample preparation (SP3) protocol, which is well suited for the processing of quantity-limited samples.
Do vegetarians feel bad? Examining the association between eating vegetarian and subjective well-being in two representative samples
2020
Abstract Research on the relationship between vegetarianism and subjective well-being (SWB) has produced inconsistent results, which may partly be due to small sample sizes and divergent operationalizations of well-being. For these reasons, the present study aimed to thoroughly examine this association in two large representative samples from Germany (Study 1: N = 12,905, including 665 vegetarians) and Australia (Study 2: N = 15,532, including 383 vegetarians) using a consensual conceptualization of SWB (composed of an affective component, i.e., positive and negative affect, and a cognitive component, i.e., life satisfaction). Results of t-tests showed that vegetarians reported slightly hig…
Building an Optimal WSD Ensemble Using Per-Word Selection of Best System
2006
In Senseval workshops for evaluating WSD systems [1,4,9], no one system or system type (classifier algorithm, type of system ensemble, extracted feature set, lexical knowledge source etc.) has been discovered that resolves all ambiguous words into their senses in a superior way. This paper presents a novel method for selecting the best system for target word based on readily available word features (number of senses, average amount of training per sense, dominant sense ratio). Applied to Senseval-3 and Senseval-2 English lexical sample state-of-art systems, a net gain of approximately 2.5 – 5.0% (respectively) in average precision per word over the best base system is achieved. The method c…
Oxidative stability of virgin olive oil: evaluation and prediction with an adaptive neuro-fuzzy inference system (ANFIS).
2019
Background An adaptive neuro-fuzzy inference system (ANFIS) was employed to predict the oxidative stability of virgin olive oil (VOO) during storage as a function of time, storage temperature, total polyphenol, α-tocopherol, fatty acid profile, ultraviolet (UV) extinction coefficient (K268 ), and diacylglycerols (DAGs). Results The mean total quantities of polyphenols and DAGs were 1.1 and 1.9 times lower in VOOs stored at 25 °C than in the initial samples, and the mean total quantities of polyphenols and DAGs were 1.3 and 2.26 times lower in VOOs stored at 37 °C than in the initial samples, respectively. In a single sample, α-tocopherol was reduced by between 0.52 and 0.91 times during sto…
Altruistic leadership and affiliative humor's role on service innovation: Lessons from Spanish public hospitals.
2018
Despite literature identifies aspects that might promote innovation, the relationship between the leadership style and nurses' innovative behavior still remains unclear, and little research has provided evidence of this. To help advance in knowledge of effects of leadership on nurses' innovative behavior, we researched the effect of altruistic leadership on nurses' innovative behavior. In addition, the mediating role of affiliative humor in the relationship between altruistic leadership and nurses' innovative behavior was examined. Questionnaire survey method was followed with a sample of 324 nurses working in public hospitals in Spain. We used structural equation models, to check the resea…
Double validation of translated questionnaires in international studies
2018
Background Study questionnaires developed in one country can be translated and used for similar studies in other countries. The main problem of this process is that people speaking different languages can understand the meaning of some questions differently due to disparate mentalities and backgrounds. To avoid a misunderstanding, to perform correct conclusions in accordance to the mentality of each country, and to permit the comparison of results in different countries, the intellectual validation of questionnaires is widely used. The main problem of such a validation is its qualitative nature that can be biased by the subjective approach of the investigator. Therefore, there is a necessit…