Search results for "SCAR"

showing 10 items of 914 documents

SCAR-based real time PCR to identify a biocontrol strain (T1) of Trichoderma atroviride and study its population dynamics in soils.

2006

Strains of Trichoderma spp. are known for their antagonistic properties against plant pathogens, some are already on the market, others are under development. In order to launch a strain on the market its perfect identification at the species and strain levels is needed. The aim of this study is to (i) design a SCAR marker for specific identification of strain T1 of Trichoderma atroviride and (ii) monitor population dynamics of this strain in soil by real time PCR. A primer pair targeting a 141-bp fragment enabled specific detection of this strain without cross detection of autochthonous populations of Trichoderma in several field soils. In two soils, population dynamics assessed by real ti…

Microbiology (medical)PopulationBiologycomplex mixturesMicrobiologyPolymerase Chain Reactionlaw.inventionlawREAL-TIME PCReducationDNA FungalMolecular BiologyPolymerase chain reactionSoil MicrobiologyTrichodermaeducation.field_of_studyStrain (chemistry)business.industryFungal geneticsfood and beveragesFungi imperfectiSequence Analysis DNAbiology.organism_classificationDNA FingerprintingSOILSBiotechnologyRandom Amplified Polymorphic DNA TechniquePOPULATION DYNAMICSSCAR[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyTrichodermabusinessSoil microbiologySpecific identificationJournal of microbiological methods
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Hybridization of mouse lemurs: different patterns under different ecological conditions

2011

Abstract Background Several mechanistic models aim to explain the diversification of the multitude of endemic species on Madagascar. The island's biogeographic history probably offered numerous opportunities for secondary contact and subsequent hybridization. Existing diversification models do not consider a possible role of these processes. One key question for a better understanding of their potential importance is how they are influenced by different environmental settings. Here, we characterized a contact zone between two species of mouse lemurs, Microcebus griseorufus and M. murinus, in dry spiny bush and mesic gallery forest that border each other sharply without intermediate habitats…

Microcebus murinusEvolutionMolecular Sequence DataPopulationIntrogressionLemurCheirogaleidaeDNA MitochondrialLinkage DisequilibriumHybrid zonebiology.animalMadagascarQH359-425AnimalseducationEcosystemPhylogenyEcology Evolution Behavior and SystematicsDNA Primerseducation.field_of_studyBase SequenceModels GeneticbiologyEcologyBayes TheoremSequence Analysis DNAbiology.organism_classificationGenetics PopulationHaplotypesHabitatEvolutionary biologyHybridization GeneticCheirogaleidaeMicrocebus griseorufusMicrosatellite RepeatsResearch ArticleBMC Evolutionary Biology
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Analysis of intermittent supply systems in water scarcity conditions and evaluation of the resource distribution equity indices

2007

Generally, urban water distribution shortage situations are solved by introducing discontinuous service and rationing the available water resources. This approach is widely adopted, not only in developing countries but also in developed ones, for solving short term scarcity conditions which can be caused by unpredicted drought periods. Intermittent distribution has the advantage of requiring small financial efforts but it leads to network operating conditions that are very far from the usual design ones. With the aim to analyse and describe the water supply network behaviour in intermittent conditions, a network hydraulic model has been set up in which both user and manager dependent regula…

Microeconomicsdistribution network performance water scarcity conditions intermittent distributionNatural resource economicsEconomicsEquity (finance)Resource distributionWater scarcity
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Nutritional Profiling and the Value of Processing By-Products from Gilthead Sea Bream (

2019

Fish processing industries generate a large volume of discards. In order to fulfil with the principles of a sustainable circular economy, it is necessary to maintain aquaculture by-products in the food chain through the production of high-value biomolecules that can be used as novel ingredients. In this study, we try to give value to the gilthead sea bream by-products, evaluating the composition and the nutritional value of the muscle and six discards commonly obtained from the fish processing industry (fishbone, gills, guts, heads, liver, and skin), which represent ≈ 61% of the whole fish. Significant differences were detected among muscle and by-products for fatty acid and amino acid prof…

Mineralsamino acidsFood HandlingDissectionFatty Acidsfish discardsFishesAquacultureNutrientsSea BreamArticlemineral compositionfatty acid profileBody CompositionAnimalsvaluable compoundsNutritive ValueMarine drugs
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Missed abortion etioloģija, gestācijas laiks un terapijas izvēle

2021

Ievads: Nenoticis aborts (NA) ir embrija vai augļa bojāeja pirms pilnām 22 gestācijas nedēļām bez augļa olas izstumšanas no dzemdes. Tā ir bieži sastopama agrīnas grūtniecības komplikācija, kas skar sievietes reproduktīvā vecumā gan Latvijā, gan visā pasaulē. NA ir sastopams aptuveni 15 % no visām klīniski diagnosticētajām grūtniecībām un sastāda aptuveni 30 % no kopējā abortu skaita un tas ir otrais biežāk reģistrētais aborta veids Latvijas slimnīcās pēc legāla aborta. Pētījuma mērķis: Izpētīt vai pacientēm, kuras saņēma misoprostolu pēc shēmas, kas rekomendēta NA ārstēšanai pēc FIGO, pozitīvs ārstēšanas rezultāts tiks sasniegts biežāk, nekā pacientēm, kas misoprostolu saņēma pēc citas lie…

Missed abortionDzemdes dobuma abrāzijaNenoticis abortsMiscarriageMisoprostolsMedicīna
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What is the form of muscimol from fly agaric mushroom (Amanita muscaria) in water? An insight from NMR experiment supported by molecular modeling.

2020

ARTYKUŁ Z BADAŃ SPECJALNYCH

Models MolecularMagnetic Resonance SpectroscopyAmanita010402 general chemistry01 natural scienceschemistry.chemical_compoundReceptors GABAComputational chemistryMoleculeHumansGeneral Materials ScienceGABA-A Receptor Antagonistsindirect spin‐spin coupling constantsNMR in waterDensity Functional TheoryCarbon Isotopesbiology010405 organic chemistryAgaricmolecular modelingMuscimolChemical shiftWaterGeneral ChemistryCarbon-13 NMRbiology.organism_classification0104 chemical sciencesSolventnervous systemchemistryMuscimolGIAO NMRZwitterionProtonsAmanita muscariaMagnetic resonance in chemistry : MRCREFERENCES
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Obstetrics outcomes after complete and partial molar pregnancy: Review of the literature and meta-analysis.

2021

The hydatidiform mole is a rare gynecological disease rising from the trophoblastic. Post-molar pregnancies have an extremely variable course, varying from repeated abortions, stillbirths, preterm births, live births, or recurring in further molar pregnancies. Literature on obstetric outcomes following molar pregnancy is poor, often including monocentric studies, and with data collected from national databases. This review and meta-analysis aim to analyze the obstetric outcomes after conservative management of complete (CHM) and partial (PHM) molar pregnancies. The meta-analysis was performed following the Meta-analysis Of Observational Studies in Epidemiology (MOOSE) and the preferred repo…

Molarmedicine.medical_specialtyMiscarriage03 medical and health sciences0302 clinical medicineMolar pregnancyPregnancyEpidemiologymedicineHumans030212 general & internal medicinePregnancy030219 obstetrics & reproductive medicineObstetricsbusiness.industryInfant NewbornObstetrics and GynecologyHydatidiform Molemedicine.diseaseConfidence intervalObstetricsSystematic reviewReproductive MedicineMeta-analysisUterine NeoplasmsPremature BirthFemaleNeoplasm Recurrence LocalbusinessEuropean journal of obstetrics, gynecology, and reproductive biology
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Paradox between malnutrition and nutritional richness of foods in Madagascar: the example of Moringa oleifera

2013

Madagascar has two faces: a developing country with a high percentage of population under poverty scale, and a country rich in natural resources. Only few studies examined this paradox between malnutrition prevalence and food availability. In other developing countries Moringa oleifera (MO) was proposed as a potential solution for its high nutritional value. In Madagascar this plant grows in the wild.The present research investigated why MO leaves are not part of Malagasy diet and how it could be introduce as daily food. A first study examined the actual nutritional composition of Malagasy MO leaves, and a second one the perceived nutritional value of these leaves by Malagasy people.Results …

Moringa oleifera[SDV.AEN] Life Sciences [q-bio]/Food and Nutritionnutrition[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionMadagascarprotein[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
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Multiple overseas dispersal in amphibians

2003

Amphibians are thought to be unable to disperse over ocean barriers because they do not tolerate the osmotic stress of salt water. Their distribution patterns have therefore generally been explained by vicariance biogeography. Here, we present compelling evidence for overseas dispersal of frogs in the Indian Ocean region based on the discovery of two endemic species on Mayotte. This island belongs to the Comoro archipelago, which is entirely volcanic and surrounded by sea depths of more than 3500 m. This constitutes the first observation of endemic amphibians on oceanic islands that did not have any past physical contact to other land masses. The two species of frogs had previously been tho…

MovementBiogeographyMolecular Sequence DataMantellidaeBiologyphylogenyDNA MitochondrialComorosGeneral Biochemistry Genetics and Molecular BiologyEvolution MolecularAmphibiaddc:570MadagascarVicarianceAnimalsIndian OceanPhylogenybiogeographyDNA PrimersGeneral Environmental ScienceLikelihood FunctionsBase SequenceGeographyModels GeneticGeneral Immunology and MicrobiologyEcologySequence Analysis DNAGeneral MedicineSalt waterBiological dispersalAnuraGeneral Agricultural and Biological SciencesResearch Article
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Discounted Prototyping of Virtual Reality Solutions for Science Education

2017

Science education requires experimental work for learners to achieve the intended learning outcomes, which sometimes involve abstract concepts by nature. Advances in digital technology can help learners to undertake experimental work, which would otherwise be costly, either due to the scarcity of laboratory equipment or safety requirements. However, one of the major concerns resides into how to design usable learning solutions from the users’ perspective. That would require the involvement of users at all stages of design and development process, even if the technology itself could still be in its infancy. It can be very helpful to present the new design concepts to potential users at a ver…

MultimediaComputer scienceProcess (engineering)business.industrymedia_common.quotation_subjectVirtual realityUSablecomputer.software_genreScience educationTest (assessment)ScarcityUser experience designHuman–computer interactionDesign processbusinesscomputermedia_common
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