Search results for "SCID"

showing 10 items of 252 documents

Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells

2010

Glioblastoma is a highly angiogenetic malignancy, the neoformed vessels of which are thought to arise by sprouting of pre-existing brain capillaries. The recent demonstration that a population of glioblastoma stem-like cells (GSCs) maintains glioblastomas indicates that the progeny of these cells may not be confined to the neural lineage. Normal neural stem cells are able to differentiate into functional endothelial cells. The connection between neural stem cells and the endothelial compartment seems to be critical in glioblastoma, where cancer stem cells closely interact with the vascular niche and promote angiogenesis through the release of vascular endothelial growth factor(VEGF) and str…

EndotheliumAngiogenesisTransplantation HeterologousSettore MED/27 - NEUROCHIRURGIAMice TransgenicMice SCIDBiologyModels BiologicalMiceVasculogenesisNeural Stem CellsMice Inbred NODCell Line TumormedicineAnimalsHumansCell LineageVasculogenic mimicryglioblastoma tumor vascularizationIn Situ Hybridization FluorescenceChromosome AberrationsMultidisciplinaryNeovascularization PathologicEndothelial CellsCell DifferentiationVascular endothelial growth factor BEndothelial stem cellVascular endothelial growth factor Amedicine.anatomical_structureVascular endothelial growth factor CTumor Markers BiologicalImmunologyCancer researchEndothelium VascularGlioblastomaNeoplasm TransplantationNature
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On the induced drag reduction due to propeller-wing interaction

1995

AbstractThe aerodynamic interaction between a wing and a couple of propellers in tractor configuration is investigated by means of a model based on the lifting line concept and under the assumption of quasi-steady incompressible motion of inviscid fluid. The ways the propellers influence the wing performances, particularly the induced drag, are analysed. It turns out that the lift increase and the possible drag reduction depend strongly on the direction of the blade rotation and on the propeller distances from midspan, and result from two main effects: the direct propeller induction on the wing and the modification of the lift distribution along the span. An additional nonlinear (mixed) con…

EngineeringWingLift-induced dragbusiness.industryPropellerAerospace EngineeringMechanical engineeringAerodynamicsMechanicsRotationLift (force)Inviscid flowDragbusinessThe Aeronautical Journal
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On numerical simulation of electromagnetic field effects in the combustion process

2018

This paper deals with a simplified model taking into account the interplay of compressible, laminar, axisymmetric flow and the electrodynamical effects due to Lorentz force’s action on the combustion process in a cylindrical pipe. The combustion process with Arrhenius kinetics is modelled by a single step exothermic chemical reaction of fuel and oxidant. We analyze non-stationary PDEs with 6 unknown functions: the 3 components of velocity, density, concentration of fuel and temperature. For pressure the ideal gas law is used. For the inviscid flow approximation ADI method is used. Some numerical results are presented.

Exothermic reactionElectromagnetic fieldMaterials science01 natural sciences010305 fluids & plasmasPhysics::Fluid Dynamicssymbols.namesakeLorentz forceInviscid flow0103 physical sciencesQA1-9390101 mathematicsPhysics::Chemical PhysicsIdeal gas law010102 general mathematicsaxisymmetric flowArrhenius kineticsLaminar flowMechanicslaminarAlternating direction implicit methodModeling and SimulationCompressibilitysymbolsLorentz forceAnalysisMathematicscompressibleMathematical Modelling and Analysis
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Enhancerless Cytomegalovirus Is Capable of Establishing a Low-Level Maintenance Infection in Severely Immunodeficient Host Tissues but Fails in Expon…

2010

ABSTRACT Major immediate-early transcriptional enhancers are genetic control elements that act, through docking with host transcription factors, as a decisive regulatory unit for efficient initiation of the productive virus cycle. Animal models are required for studying the function of enhancers paradigmatically in host organs. Here, we have sought to quantitatively assess the establishment, maintenance, and level of in vivo growth of enhancerless mutants of murine cytomegalovirus in comparison with those of an enhancer-bearing counterpart in models of the immunocompromised or immunologically immature host. Evidence is presented showing that enhancerless viruses are capable of forming restr…

Gene Expression Regulation ViralMutantImmunology/dk/atira/pure/subjectarea/asjc/2400/2406CytomegalovirusMice SCIDBiologyMicrobiologyVirusImmunocompromised HostMiceExponential growthIn vivoVirologyAnimalsHumans/dk/atira/pure/subjectarea/asjc/2400/2403EnhancerTranscription factorMice Inbred BALB CVirologyGenome Replication and Regulation of Viral Gene ExpressionEnhancer Elements GeneticInsect ScienceCytomegalovirus InfectionsHost-Pathogen InteractionsCytomegalovirus infections
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Severe hepatic injury in interleukin 18 (IL-18) transgenic mice: a key role for IL-18 in regulating hepatocyte apoptosis in vivo.

2004

Background: Interleukin 18 (IL-18) is a cytokine with pleiotropic activity that augments T helper 1 responses and cytotoxic activity of natural killer cells. Methods: To assess the function of IL-18 in vivo, we generated IL-18 transgenic (IL-18 Tg) mice under the control of a CD2 promoter/enhancer construct. Results: Macroscopically, IL-18 Tg mice showed reduced relative liver weight compared with wild-type littermates. TUNEL assays demonstrated increased hepatocyte apoptosis, and primary hepatocytes isolated from IL-18 Tg mice exhibited an increased spontaneous apoptosis rate. Furthermore, cross linking of Fas increased significantly the apoptosis rate in hepatocytes isolated from wild- ty…

Genetically modified mousemedicine.medical_specialtyTransgeneT-LymphocytesApoptosisMice TransgenicMice SCIDBiologyTransfectionTranslocation GeneticInterferon-gammaMiceIn vivoInternal medicinemedicineCytotoxic T cellAnimalsfas ReceptorL-SelectinCells CulturedLiver injuryTumor Necrosis Factor-alphaGastroenterologyInterleukin-18NF-kappa BOrgan Sizemedicine.diseaseAdoptive TransferEndocrinologymedicine.anatomical_structureLiverApoptosisHepatocyteLymphocyte TransfusionCancer researchHepatocytesInterleukin 18Gut
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Ag-NOR and C-banding analysis of spermatocyte chromosomes ofClavelina lepadiformis(Ascidiacea, Aplousobranchiata)

1991

SUMMARYChromosome number n = 9 and 2n = 18 for Clavelina lepadiformis (Ascidiacea, Aplousobranchiata) from the Gulf of Palermo have been determined. Silver staining analysis of testicular cells reveals that within-individual variability in NOR banding patterns is present. Using the C-banding procedure, a very impressive heterochromatin amount seems to characterize the chromosome set of this species.

Geneticsmedicine.medical_specialtyHeterochromatinCytogeneticsChromosomeSpermatocyteBiologybiology.organism_classificationMolecular biologySilver stainmedicine.anatomical_structureGeneticsmedicineClavelina lepadiformisNucleolus organizer regionGeneral Agricultural and Biological SciencesAscidiaceaCaryologia
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Data from: Phylogenomics of Lophotrochozoa with consideration of systematic error

2021

Phylogenomic studies have improved understanding of deep metazoan phylogeny and show promise for resolving incongruences among analyses based on limited numbers of loci. One region of the animal tree that has been especially difficult to resolve, even with phylogenomic approaches, is relationships within Lophotrochozoa (the animal clade that includes molluscs, annelids, and flatworms among others). Lack of resolution in phylogenomic analyses could be due to insufficient phylogenetic signal, limitations in taxon and/or gene sampling, or systematic error. Here, we investigated why lophotrochozoan phylogeny has been such a difficult question to answer by identifying and reducing sources of sys…

Helobdella robustaGlycera dibranchiataMytilus edulisAnnelidaEntalina tetragonaLeptochiton asellusCerebratulus marginatusLoxosomella cf. viviparaGraptacme eboreaLineus longissimusmedicine and health careClymenella torquataRuditapes philippinarumNucella lapillusHaliotis rufescenslong branch attractionPlatyzoaBarentsia gracilisPriapulus caudatusLineus ruberAlitta virenssaturationProchaetoderma californicumLife SciencesPinctada fucataSchistosoma mansoniPolyzoaCephalothrix hongkongensisRhyssoplax olivaceusLoxosoma pectinaricolaPhascolosoma agassiziiAdineta vagaDrosophila melanogasterEntoproctaBugula neritinaPhoronis vancouverensisMedicineNovocrania anomalaVillosa lienosaDaphnia pulexSagitta sp.Pectinaria gouldiiSymbion americanusNuculana pernulaSepia esculentaEnucula tenuisSolemya velumLineus lacteusTubulanus polymorphus-StruckGnathostomula paradoxaBoccardia proboscideaMacellomenia schanderiLaevipilina hyalinaTubulanus polymorphus-HalanychBryozoaPomatoceros lamarckiiSepioteuthis lessonianaParanemertes peregrinaMalacobdella grossaHemithiris psittaceaLeptochiton rugatusTrochozoaBrachionus plicatilisSpathoderma clenchiLaqueus californicusPatella vulgataLottia giganteaCrepidula fornicataPhoronidaAplysia californicaGlottidia pyramidataPhoronis psammophilaSchmidtea mediterraneaAlexandromenia crassaBrachiopodaMegadasys sp.Octopus vulgarisCapitella teletaNeomenia carinatacompositional heterogeneityNemerteaPhenacolepas pulchellaGadila tolmieiMolluscaMacrodasys sp.Crassostrea gigasPedicellina cernuaTaenia pisiformisDosidicus gigasCephalothrix linearisSpiralia
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Data from: Phylogenomics of Lophotrochozoa with consideration of systematic error

2016

Phylogenomic studies have improved understanding of deep metazoan phylogeny and show promise for resolving incongruences among analyses based on limited numbers of loci. One region of the animal tree that has been especially difficult to resolve, even with phylogenomic approaches, is relationships within Lophotrochozoa (the animal clade that includes molluscs, annelids, and flatworms among others). Lack of resolution in phylogenomic analyses could be due to insufficient phylogenetic signal, limitations in taxon and/or gene sampling, or systematic error. Here, we investigated why lophotrochozoan phylogeny has been such a difficult question to answer by identifying and reducing sources of sys…

Helobdella robustaGlycera dibranchiataMytilus edulisAnnelidaEntalina tetragonaLeptochiton asellusCerebratulus marginatusLoxosomella cf. viviparaGraptacme eboreaLineus longissimusmedicine and health careClymenella torquataRuditapes philippinarumNucella lapillusHaliotis rufescenslong branch attractionPlatyzoaBarentsia gracilisPriapulus caudatusLineus ruberAlitta virenssaturationProchaetoderma californicumPinctada fucataSchistosoma mansoniLife sciencesPolyzoaCephalothrix hongkongensisRhyssoplax olivaceusLoxosoma pectinaricolaPhascolosoma agassiziiAdineta vagaDrosophila melanogasterEntoproctaBugula neritinaPhoronis vancouverensisMedicineNovocrania anomalaVillosa lienosaDaphnia pulexSagitta sp.Pectinaria gouldiiSymbion americanusNuculana pernulaSepia esculentaEnucula tenuisSolemya velumLineus lacteusTubulanus polymorphus-StruckGnathostomula paradoxaBoccardia proboscideaMacellomenia schanderiLaevipilina hyalinaTubulanus polymorphus-HalanychBryozoaPomatoceros lamarckiiSepioteuthis lessonianaParanemertes peregrinaMalacobdella grossaHemithiris psittaceaLeptochiton rugatusTrochozoaBrachionus plicatilisSpathoderma clenchiLaqueus californicusPatella vulgataLottia giganteaCrepidula fornicataPhoronidaAplysia californicaGlottidia pyramidataPhoronis psammophilaSchmidtea mediterraneaAlexandromenia crassaBrachiopodaMegadasys sp.Octopus vulgarisCapitella teletaNeomenia carinatacompositional heterogeneityNemerteaPhenacolepas pulchellaGadila tolmieiMolluscaMacrodasys sp.Crassostrea gigasPedicellina cernuaTaenia pisiformisDosidicus gigasCephalothrix linearisSpiralia
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Human CD8+ memory and EBV-specific T cells show low alloreactivity in vitro and in CD34+ stem cell–engrafted NOD/SCID/IL-2Rγcnull mice

2013

Current strategies in cellular immunotherapy of cancer and viral infections include the adoptive transfer of T cell receptor (TCR) and chimeric antigen receptor engineered T cells. When using transient RNA expression systems in clinical studies, multiple infusions with receptor-redirected T cells appear necessary. However, in allogeneic hematopoietic stem-cell transplantation, repeated transfer of donor-derived T cells increases the risk of alloreactive graft-versus-host disease. We investigated naive-derived (T N ), memory-derived (T M ), and Epstein Barr virus-specific (T EBV ) CD8 + T cell subsets for alloreactivity upon redirection with RNA encoding a cytomegalovirus-specific model TCR.…

Herpesvirus 4 HumanCancer ResearchT-LymphocytesT cellAntigens CD34Mice SCIDStreptamerCD8-Positive T-LymphocytesBiologyImmunotherapy AdoptiveMiceInterleukin 21GeneticsmedicineAnimalsHumansTransplantation HomologousCytotoxic T cellIL-2 receptorAntigen-presenting cellMolecular BiologyInterleukin 3Histocompatibility TestingHematopoietic Stem Cell TransplantationCell BiologyHematologyNatural killer T cellmedicine.anatomical_structureImmunologyCancer researchImmunologic MemoryInterleukin Receptor Common gamma SubunitExperimental Hematology
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Periodic massloss from viscous accretion flows around black holes

2014

We investigate the behaviour of low angular momentum viscous accretion flows around black holes using Smooth Particle Hydrodynamics (SPH) method. Earlier, it has been observed that in a significant part of the energy and angular momentum parameter space, rotating transonic accretion flow undergoes shock transition before entering in to the black hole and a part of the post-shock matter is ejected as bipolar outflows, which are supposed to be the precursor of relativistic jets. In this work, we simulate accretion flows having injection parameters from the inviscid shock parameter space, and study the response of viscosity on them. With the increase of viscosity, shock becomes time dependent …

High Energy Astrophysical Phenomena (astro-ph.HE)Shock wavePhysicsAngular momentumShock (fluid dynamics)Astrophysics::High Energy Astrophysical PhenomenaFOS: Physical sciencesAstronomy and AstrophysicsAstrophysicsMechanicsAccretion (astrophysics)Physics::Fluid DynamicsSmoothed-particle hydrodynamicsViscosityAstrophysical jetSpace and Planetary ScienceInviscid flowAstrophysics::Solar and Stellar AstrophysicsAstrophysics - High Energy Astrophysical PhenomenaAstrophysics::Galaxy Astrophysics
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