Search results for "SCOPE"
showing 10 items of 2420 documents
Cryo-negative staining
1998
Abstract A procedure is presented for the preparation of thin layers of vitrified biological suspensions in the presence of ammonium molybdate, which we termcryo-negative staining. The direct blotting of sample plus stain solution on holey carbon supports produces thin aqueous films across the holes, which are routinely thiner than the aqueous film produced by conventional negative staining on a continuous carbon layer. Because of this, a higher than usual concentration of negative stain (ca. 16% rather than 2%) is required for cryo-negative staining in order to produce an optimal image contrast. The maintenance of the hydrated state, the absence of adsorption to a carbon film and associate…
Small-angle X-ray scattering reveals differences between the quaternary structures of oxygenated and deoxygenated tarantula hemocyanin
1996
Small-angle X-ray scattering (SAXS) curves have been recorded for the oxygenated and deoxygenated states of the 4 x 6-meric hemocyanin from the tarantula Eurypelma californicum. A comparison of the curves shows that the quaternary structures of the two states are different by three criteria, which all indicate that the hemocyanin is less compact in the oxygenated compared to the deoxygenated form: (a) The radius of gyration is 8.65 +/- 0.05 nm for the deoxy- and 8.80 +/- 0.05 nm for the oxy-form. (b) The maximum particle dimension amounts to 25.0 +/- 0.5 nm for the deoxy- and to 27.0 +/- 0.5 nm for the oxy-form. (c) A dip in the intramolecular distance distribution function p(r) is more pro…
Wild-type Cu/Zn superoxide dismutase (SOD1) does not facilitate, but impedes the formation of protein aggregates of amyotrophic lateral sclerosis cau…
2009
Aggregation of Cu/Zn superoxide dismutase (SOD1) is a hallmark of a subset of familial amyotrophic lateral sclerosis (ALS) cases. The expression of wild-type SOD1 [SOD(hWT)] surprisingly exacerbates the phenotype of mutant SOD1 in vivo. Here we studied whether SOD1(hWT) may affect mutant SOD1 aggregation by employing fluorescence microscopy techniques combined with lifetime-based Förster resonance energy transfer (FRET). Only a very minor fraction of SOD1(hWT) was observed in aggregates induced by mutant SOD1(G37R), SOD1(G85R) or SOD1(G93C). Quite in contrast, co-expression of SOD(hWT) reduced the amount of mutant SOD1 in the aggregate fraction. Furthermore, we did not detect endogenous mou…
Electronic and mechanical characterization of self-assembled alkanethiol monolayers by scanning tunneling microscopy combined with interaction-force-…
1993
We have used scanning tunneling microscopy to study self-assembled monolayers of mercaptohexadecanol in ultrigh vacuum. In addition to tunneling, the interaction force gradient acting between tip and sample was measured. Analysis of the force-gradient data shows that the tip is in mechanical contact with the surface of the monolayer which, in turn, is elastically compressed. The lateral dimensions of the mechanical contact are substantially (approximately five times) larger than the width of the tunneling-current filament. The results suggest that the compression of the monolayer constitutes an integral part of tunneling through the molecules
Socially Critical Humor : Discussing Humor with Erich Fromm and Theodor W. Adorno
2017
This article brings Erich Fromm and Theodor W. Adorno back into dialogue by discussing the cultural phenomena of humor and laughter based on their theoretical writings. I argue that what is typically considered socially critical humor, like offensive jokes or harsh satire, often fails to meet the preconditions of criticism in the light of Adorno’s and Fromm’s thinking. Humor, to be socially critical, has to be life-affirmative and non-positional, and it has to challenge the limits of humor. It is also claimed that in this scope, humor cannot be instrumental.
Calcifying zooplankton standing stocks and in the North Pacific from the R/V Kilo Moana cruise KM1712
2022
This dataset compiles the standing stocks (ind/m³), the integrated standing stocks (ind/m²) and the integrated CaCO3 standing stocks (mg/m²) for three groups of zooplanktonic calcifying organisms: pteropods, heteropods and foraminifers. The organisms were collected by oblique towing (Ø 0.5 m, 90 μm mesh size, SeaGear mechanical flowmeter) in the North Pacific between Hawaii and the Gulf of Alaska during the R/V Kilo Moana cruise KM1712 in August 2017. The sampling strategy was designed to capture an integrated sample of all foraminifers, pteropods and heteropods from juveniles to adults living throughout the upper water column. Pteropods and heteropods were quantified and shell diameter mea…
Broadening the concept of expectations in public relations
2015
Expectations have been connected to many central concepts of public relations research, yet, definitions of what is meant by expectations are lacking. This article aims to broaden the understanding of expectations by taking into account their multi-dimensional nature, suggesting that there are several explanations to expectations depending on what the expectation is based on. We suggest that in organizational context expectations are two-fold assessments of what is considered good or desirable (expectation tone ranging from positive to negative) and the confidence placed in the organization (organization-specific context ranging from high to low confidence). As a result of the conceptual re…
Outcomes‐based measurement of public access computing in public libraries
2010
PurposeThis paper seeks to present a methodology for measuring the outcomes of public access computing as well as presenting and comparing the main results of baseline studies conducted in Lithuania and Latvia.Design/methodology/approachBoth baseline studies use a combination of quantitative and qualitative methods and both include multi‐perspective, longitudinal design and nation‐wide samples.FindingsThe benefits that users can gain from using PAC in public libraries include access to ICT, technology training and assistance, expanded employment opportunities, conducting commercial transactions, access to and assistance with local, state and federal government electronic services, and suppo…
Authors' reply-multi-organ ultrasonography: a stethoscope for the body
2018
We read with great interest the letter from Sakka et al . (1) commenting our article (2) on comprehensive haemodynamic monitoring in patients with acute respiratory distress syndrome (ARDS) and traumatic brain injury (TBI). We agree with the authors that haemodynamic monitoring has to be implemented in patients with ARDS complicating TBI.
Nanomolar pyrophosphate detection in water and in a self-assembled hydrogel of a simple terpyridine-Zn2+ complex.
2014
A simple terpyridine-Zn(II) complex is shown to act as an efficient and highly selective fluorescent sensor for pyrophosphate in water at physiological pH. The sensor complex showed an unprecedented fluorescence response (∼500 fold increase) and a record nanomolar sensitivity (detectable fluorescent response at 20 nM and LOD ∼ 0.8 nM). It has successfully been used to stain and record confocal fluorescence microscopy images of HeLa cells. Moreover, the complex was found to self-assemble into a hydrogel which was subsequently used to coat disposable paper strips for easy, low-cost detection of pyrophosphate.