Search results for "SCOPE"
showing 10 items of 2420 documents
Structure and Deformations of Pd−Ni Core−Shell Nanoparticles
2005
International audience; Homogeneous collections of Pd−Ni core−shell nanoparticles have been prepared by decomposition of metal−organic compounds and studied by several electron microscopy techniques: transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy (HRTEM), energy-filtered microscopy (EFTEM), and by X-ray photoelectron spectroscopy (XPS). The physical and chemical properties of the Pd shell are supposed to depend on its electronic properties, which are influenced by the presence of the Ni core and by the deformation in the Pd lattice. Here, the interfacial structure of Pd/Ni and the lattice deformations in t…
Single molecule localization microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes.
2014
Several approaches have been described to fluorescently label and image DNA and chromatin in situ on the single-molecule level. These superresolution microscopy techniques are based on detecting optically isolated, fluorescently tagged anti-histone antibodies, fluorescently labeled DNA precursor analogs, or fluorescent dyes bound to DNA. Presently they suffer from various drawbacks such as low labeling efficiency or interference with DNA structure. In this report, we demonstrate that DNA minor groove binding dyes, such as Hoechst 33258, Hoechst 33342, and DAPI, can be effectively employed in single molecule localization microscopy (SMLM) with high optical and structural resolution. Upon ill…
The effect of arabinofuranosyl-cytosine upon the synthesis of herpesvirus hominis Electron microscopic observations in relation to viral DNA-synthesis
1972
The paper describes experiments about the degree of dependency of capsid, envelope and antigen synthesis byherpesvirus hominis upon viral DNA synthesis. The DNA synthesis has been blocked by different doses of Ara-C and the remnant DNA-synthesis has been measured by [3H]-thymidine incorporation after CsCl-density gradient centrifugation. Electron microscopic studies were done in parallel after incubation of infected cells with different doses of Ara-C. Finally, antigens were prepared after infection without and with added Ara-C. Increasing amounts of Ara-C inhibited the synthesis of viral DNA and infective particles. 1.5 μg Ara-C reduced the remaining incorporation into DNA to about less th…
Detection of cytokeratin dynamics by time-lapse fluorescence microscopy in living cells.
1999
To monitor the desmosome-anchored cytokeratin network in living cells fusion protein HK13-EGFP consisting of human cytokeratin 13 and the enhanced green fluorescent protein was stably expressed in vulvar carcinoma-derived A-431 cells. It is shown for A-431 subclone AK13-1 that HK13-EGFP emits strong fluorescence in fixed and living cells, being part of an extended cytoplasmic intermediate filament network that is indistinguishable from that of parent A-431 cells. Biochemical, immunological and ultrastructural analyses demonstrate that HK13-EGFP behaves identically to the endogenous cytokeratin 13 and is therefore a reliable in vivo tag for this polypeptide and the structures formed by it. T…
Design of New Resonant Haloscopes in the Search for the Dark Matter Axion: A Review of the First Steps in the RADES Collaboration
2022
This article belongs to the Special Issue Studying the Universe from Spain.
Protection of Personal Data and Human Rights between the ECHR and the EU Legal Order
2020
The present paper deals with the relationship between privacy and data protection, having regard to the European Union and the European Convention of Human Rights. Such an analysis moves from the normative context (art. 8 ECHR and art. 7 and 8 of the Charter of Fundamental Rights of the European Union). Moreover, the case law of the Court of Justice of the European Union and of the European Court of Human RIghts is taken into account, in order to enlighten the dfferent scopes of privacy and data protection.
Composite Scaffolds with a Hydrohyapatite Spatial Gradient for Osteochondral Defect Repair
2018
Osteochondral defects derived by traumatic injury or aging related disease are often associated with severe joint pain and progressive loss of joint functions for millions of people worldwide and represent a major challenge for the orthopedic community. Tissue engineering offers new therapeutic approach to repair the osteochondral defects, through the production of scaffolds manufactured to mimic their complex architecture, which consists of cartilage and bone layers. Composite scaffolds based on a PLLA polymeric matrix containing hydroxyapatite (HA) as a filler were prepared through a modified thermally induced phase separation (TIPS) protocol. A suspension was prepared by adding sieved HA…
GPS Monitoring of the Scopello (Sicily, Italy) DGSD Phenomenon: Relationships Between Surficial and Deep-Seated Morphodynamics
2015
The Scopello area, which is located along the north-western Tyrrhenian coastal sector of the Sicilian chain (Italy), is widely affected by Deep-seated Gravitational Slope Deformation (DGSD) phenomena, which are mainly the result of a geomorphologic setting marked by the outcropping of an overthrust plan, limiting a brittle fractured carbonate slab, laid onto a ductile marly-clayey substratum. Due to the very advanced stage of the deformation phenomena, a coupled morphodynamic style has established between shallow landslides and DGSD phenomena, affecting the exhumed ductile substratum and the overlaying rigid dismantled slab, respectively. A GPS network was realized for monitoring the Scopel…
Continuous-Variable Tomography of Solitary Electrons
2019
A method for characterising the wave-function of freely-propagating particles would provide a useful tool for developing quantum-information technologies with single electronic excitations. Previous continuous-variable quantum tomography techniques developed to analyse electronic excitations in the energy-time domain have been limited to energies close to the Fermi level. We show that a wide-band tomography of single-particle distributions is possible using energy-time filtering and that the Wigner representation of the mixed-state density matrix can be reconstructed for solitary electrons emitted by an on-demand single-electron source. These are highly localised distributions, isolated fro…
Early bone healing around implant surfaces treated with variations in the resorbable blasting media method. A study in rabbits.
2010
Objective: this study aimed to histomorphologically and histomorphometrically evaluate the in vivo response to three variations in the resorbable blasting media (RBM) surface processing in a rabbit femur model. Study Design: screw root form implants with 3.75 mm in diameter by 8 mm in length presenting four surfaces (n=8 each): alumina-blasted/acid-etched (AB/AE), bioresorbable ceramic blasted (TCP), TCP + acid etching, and AB/AE + TCP were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The implants were placed at the distal femur of 8 New Zeland rabbits, remaining for 2 weeks in vivo. After sacrifice, the implants were nondecalcified processed to 30 …