Search results for "SED"

showing 10 items of 21612 documents

Middle Jurassic tracks of sauropod dinosaurs in a deep karst cave in France.

2019

14 pages; International audience; Although the deep galleries of natural underground cavities are difficult to access and are sometimes dangerous, they have the potential to preserve trace fossils. Here, we report on the first occurrence of sauropod dinosaur tracks inside a karstic cave. Three trackways are preserved on the roof of the Castelbouc cave 500 m under the surface of the Causse Méjean plateau, southern France. The tracks are Bathonian in age (ca. 168–166 Ma), a crucial but still poorly known time interval in sauropod evolution. The three trackways yield sauropod tracks that are up to 1.25 m long and are therefore amongst the largest known dinosaur footprints worldwide. The trackm…

0106 biological sciences010506 paleontologyBAGA FORMATIONTrace fossilSOUTHERN FRANCE010603 evolutionary biology01 natural sciencesNatural (archaeology)Sedimentary depositional environmentPaleontology0105 earth and related environmental sciencesBASINgeographygeography.geographical_feature_categoryPaleontologyTrackwayKarstEVOLUTIONMOUNTAINSGRANDS-CAUSSES[SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/PaleontologyFOOTPRINTSPENINSULAGeologyICHNOFAUNABORNHOLM
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Paleohistology of the Cretaceous resin‐producing conifer Geinitzia reichenbachii using X‐ray synchrotron microtomography

2021

International audience; PremiseThe conifer Geinitzia reichenbachii was a common member of the Cretaceous Laurasian floras. However, the histology of G. reichenbachii leafy axes was never described in detail, and our knowledge of its paleoecology remains very limited. Using new and exquisitely preserved silicified material from the Upper Cretaceous of western France, we describe G. reichenbachii from the gross morphology to the cellular scale, then discuss paleoecological and taphonomical implications.MethodsWe examined specimens from two localities in western France (Claix and Moragne) using propagation phase-contrast X-ray synchrotron microtomography.ResultsThe cuticle and the inner tissue…

0106 biological sciences010506 paleontologyContext (language use)Plant ScienceBiology010603 evolutionary biology01 natural sciencesSedimentary depositional environmentPaleontologyGeneticsMesozoicleafy twigsEcosystemEcology Evolution Behavior and Systematics0105 earth and related environmental sciencespaleoecophysiologyPermineralizationpermineralizationX-RaysConiferalesX-ray synchrotron microtomographX-Ray MicrotomographyGeinitziaceae15. Life on landCretaceousAmberTracheophyta[SDE]Environmental SciencesPaleobotanyTracheidPaleoecologyfossilssilicification[SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/PaleontologySynchrotronsMesozoic paleobotanyAmerican Journal of Botany
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A continuous multi-millennial record of surficial bivalve mollusk shells from the São Paulo Bight, Brazilian shelf

2014

AbstractTo evaluate the potential of using surficial shell accumulations for paleoenvironmental studies, an extensive time series of individually dated specimens of the marine infaunal bivalve mollusk Semele casali was assembled using amino acid racemization (AAR) ratios (n = 270) calibrated against radiocarbon ages (n = 32). The shells were collected from surface sediments at multiple sites across a sediment-starved shelf in the shallow sub-tropical São Paulo Bight (São Paulo State, Brazil). The resulting 14C-calibrated AAR time series, one of the largest AAR datasets compiled to date, ranges from modern to 10,307 cal yr BP, is right skewed, and represents a remarkably complete time series…

0106 biological sciences010506 paleontologySediment010603 evolutionary biology01 natural scienceslaw.inventionSedimentary depositional environmentchemistry.chemical_compoundPaleontologyOceanographyArts and Humanities (miscellaneous)chemistrylawBenthic zoneGeneral Earth and Planetary SciencesCarbonateAmino acid datingBathymetryRadiocarbon datingGeologyHolocene0105 earth and related environmental sciencesEarth-Surface ProcessesQuaternary Research
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Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine

2017

Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…

0106 biological sciences0301 basic medicineArabinoseMolecular biologylcsh:MedicineLaccasesBiochemistryBiotecnologia01 natural sciencesSubstrate Specificitylaw.inventionDatabase and Informatics Methodschemistry.chemical_compoundlawRecombinant Protein PurificationCloning MolecularAmineslcsh:Sciencechemistry.chemical_classificationMultidisciplinaryABTSbiologyOrganic CompoundsTemperatureHydrogen-Ion ConcentrationTyramineRecombinant ProteinsEnzymesChemistryRecombination-Based AssayBiochemistryPhysical SciencesRecombinant DNAElectrophoresis Polyacrylamide GelOxidation-ReductionSequence AnalysisResearch ArticleProtein PurificationBioinformaticsTyramineLibrary ScreeningDNA constructionResearch and Analysis Methods03 medical and health sciencesBacterial ProteinsSequence Motif Analysis010608 biotechnologyAmino Acid SequenceBenzothiazolesPediococcus acidilacticiLaccaseMolecular Biology Assays and Analysis TechniquesBase SequenceMolecular massLaccaseOrganic Chemistrylcsh:RChemical CompoundsBiology and Life SciencesProteinsPediococcus acidilacticiSequence Analysis DNAbiology.organism_classificationMolecular biology techniques030104 developmental biologyEnzymechemistryPlasmid ConstructionEnzymologySpectrophotometry Ultravioletlcsh:QSulfonic AcidsEnzimsProteïnesPurification TechniquesPLOS ONE
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Changes in freshwater sediment microbial populations during fermentation of crude glycerol

2020

This work was supported by the Latvian Council of Science , project NN-CARMA, project No. lzp-2018/1-0194.

0106 biological sciences0301 basic medicineFirmicutesMicroorganismlcsh:BiotechnologyMicroorganismsFirmicutes01 natural sciencesApplied Microbiology and BiotechnologyActinobacteriaButyric acid03 medical and health scienceschemistry.chemical_compound010608 biotechnologylcsh:TP248.13-248.65:NATURAL SCIENCES:Physics [Research Subject Categories]GlycerolFood sciencelcsh:QH301-705.5ClostridiumCrude glycerolbiologyFreshwater sediment microbial populations fermentationbiology.organism_classification6. Clean waterActinobacteriaqPCR030104 developmental biologychemistryMicrobial population biologylcsh:Biology (General)Biodiesel productionFermentationAnaerobic fermentationGammaproteobacteriaBiotechnologyElectronic Journal of Biotechnology
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From microbial eukaryotes to metazoan vertebrates: Wide spectrum paleo-diversity in sedimentary ancient DNA over the last ~14,500 years

2018

Most studies that utilize ancient DNA have focused on specific groups of organisms or even single species. Instead, the whole biodiversity of eukaryotes can be described using universal phylogenetic marker genes found within well-preserved sediment cores that cover the post-glacial period. Sedimentary ancient DNA samples from Lake Lielais Svētiņu, eastern Latvia, at a core depth of 1,050 cm in ~150 year intervals were used to determine phylotaxonomy in domain Eukaryota. Phylotaxonomic affiliation of >1,200 eukaryotic phylotypes revealed high richness in all major eukaryotic groups-Alveolata, Stramenopiles, Cercozoa, Chlorophyta, Charophyta, Nucletmycea, and Holozoa. The share of organisms t…

0106 biological sciences0301 basic medicineGeologic SedimentsBiodiversityChlorophyta010603 evolutionary biology01 natural sciences03 medical and health sciencesRNA Ribosomal 18SAnimals14. Life underwaterDNA AncientEcology Evolution Behavior and SystematicsGeneral Environmental ScienceTrophic levelbiologyEcologyLake ecosystemSequence Analysis DNA15. Life on landbiology.organism_classification030104 developmental biologyAncient DNAVertebratesPaleoecologyGeneral Earth and Planetary SciencesSpecies richnessCercozoaGeobiology
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Coping with the climate: cuticular hydrocarbon acclimation of ants under constant and fluctuating conditions

2018

International audience; Terrestrial arthropods achieve waterproofing by a layer of cuticular hydrocarbons (CHCs). At the same time, CHCs also serve as communication signals. To maintain waterproofing under different climate conditions, insects adjust the chemical composition of their CHC layer, but this may affect the communication via CHCs. The detailed acclimatory changes of CHCs and how these influence their physical properties are still unknown. Here, we studied acclimation in two closely related ant species with distinct CHC profiles, Myrmica rubra and Myrmica ruginodis, in response to constant or fluctuating temperature and humidity regimes. We measured how acclimation affected CHC co…

0106 biological sciences0301 basic medicineHot TemperaturePhysiologyDesiccation resistanceAcclimatizationClimateClimate Change[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]Phenotypic plasticityAquatic ScienceMyrmica rubra010603 evolutionary biology01 natural sciencesAcclimatizationDrought survivalCHCs03 medical and health sciencesSpecies SpecificityAnimalsRelative humidityMyrmica ruginodisSolid contentMicrorheologyMolecular BiologyEcology Evolution Behavior and Systematicschemistry.chemical_classificationPhenotypic plasticitybiologyAntsEcologyViscosityHumidityHumidity15. Life on landbiology.organism_classificationHydrocarbons[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology030104 developmental biologyHydrocarbonchemistry13. Climate actionInsect ScienceAnimal Science and Zoology[SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/BioclimatologyRheology[PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
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Effects of plant density and cutting-type on rooting and growth of an extensive green roof of Sedum sediforme (Jacq.) Pau in a Mediterranean environm…

2020

Abstract Species of the Sedum genus are a popular choice for green roofs as they thrive in shallow growth layers and are resilient to extreme environmental conditions; they are also easy to propagate from seed, plantlings or plant parts. The use of rooted cuttings is most widespread due to good rates of establishment. In the interest of cost reduction, the direct spreading of Sedum cuttings over the roof area, especially over large areas, has attracted attention in recent years. Considering the interest on the above method and the seeming lack of experimental data available, this study aims to further knowledge in this area. Experimental tests on direct spreading of unrooted cuttings of Sed…

0106 biological sciences0301 basic medicineMediterranean climateVegetative reproductionGreen roofHorticultureSedum sediformeCrassulaceae01 natural sciences03 medical and health sciencesCuttingground coverVegetative propagationunrooted cuttingRoofSicilybiologybiology.organism_classificationSedumSettore AGR/02 - Agronomia E Coltivazioni ErbaceeSedum sediforme (Jacq.) Pau030104 developmental biologyAgronomyDirect spreadingPlant coverEnvironmental science010606 plant biology & botany
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Sedimentary Ancient DNA (sedaDNA) Reveals Fungal Diversity and Environmental Drivers of Community Changes throughout the Holocene in the Present Bore…

2021

Fungi are ecologically important in several ecosystem processes, yet their community composition, ecophysiological roles, and responses to changing environmental factors in historical sediments are rarely studied. Here we explored ancient fungal DNA from lake Lielais Svētiņu sediment throughout the Holocene (10.5 kyr) using the ITS metabarcoding approach. Our data revealed diverse fungal taxa and smooth community changes during most of the Holocene with rapid changes occurring in the last few millennia. More precisely, plankton parasitic fungi became more diverse from the Late Holocene (2–4 kyr) which could be related to a shift towards a cooler climate. The Latest Holocene (~2 kyr) showed …

0106 biological sciences0301 basic medicineMicrobiology (medical)paleoenvironmentEnvironmental changelake sedimentsBiology010603 evolutionary biology01 natural sciencesMicrobiologyArticlepaleoecology03 medical and health sciencesfungal biodiversityVirologysedimentary ancient DNAEcosystemlcsh:QH301-705.5HolocenepaleolimnologyEcologyfungiPlankton030104 developmental biologyAncient DNABoreallcsh:Biology (General)metabarcodingPaleoecologyenvironmental driversTerrestrial ecosystemITSpaleogeneticsMicroorganisms
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Multiple shifts to open habitats in Melastomateae (Melastomataceae) congruent with the increase of African Neogene climatic aridity

2018

International audience; AimAfrican Melastomateae (Melastomataceae) comprise c. 185 species occurring in closed or open habitats throughout sub-Saharan Africa. We sought to reconstruct biogeographical and habitat history, and shifts in diversification rates of African Melastomateae using a well-sampled, dated molecular phylogeny.LocationAmericas, sub-Saharan Africa, Madagascar, SE Asia.TaxonAngiosperms, Melastomataceae, African Melastomateae.MethodsPhylogenetic relationships were estimated based on an extensive sampling of New and Old World Melastomateae, using two nuclear and three plastid markers. Divergence times were estimated in BEAST based on three calibration priors under Bayesian unc…

0106 biological sciences0301 basic medicineOld WorlddiversificationMelastomataceaeBiogeography[SDV]Life Sciences [q-bio]divergence timeNeogene010603 evolutionary biology01 natural sciences03 medical and health sciencesopen habitatsclosed habitats[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyEcology Evolution Behavior and Systematicsbiogeographyhabitat shiftsEcologybiologyEcology15. Life on landbiology.organism_classification030104 developmental biologyGeographyTaxonHabitatMolecular phylogeneticsAfricaMelastomataceaeBiological dispersalNeogenegrassland
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