Search results for "SENSORS"
showing 10 items of 343 documents
Cellular and Molecular Signatures of Oxidative Stress in Bronchial Epithelial Cell Models Injured by Cigarette Smoke Extract
2022
Exposure of the airways epithelium to environmental insults, including cigarette smoke, results in increased oxidative stress due to unbalance between oxidants and antioxidants in favor of oxidants. Oxidative stress is a feature of inflammation and promotes the progression of chronic lung diseases, including Chronic Obstructive Pulmonary Disease (COPD). Increased oxidative stress leads to exhaustion of antioxidant defenses, alterations in autophagy/mitophagy and cell survival regulatory mechanisms, thus promoting cell senescence. All these events are amplified by the increase of inflammation driven by oxidative stress. Several models of bronchial epithelial cells are used to study the molec…
Ink-Jet Printing for drug srreening on microarrays: from covalent approaches to in-liquid-droplets assays
2012
Drug screening is the complex process of retrieving chemical compounds able to modulate the activity of biological targets which are of interest for certain diseases. Conventional miniaturized drug screening technologies are based on robotic dispensers coupled with microwell arrays. However these devices require time and reagent consuming (micro-, nanoliter scale) instrumental tools, liquid handling robotics and complex detectors. Here we show a low-cost and efficient drug screening methodology based on inkjet printing for delivering molecular systems in picoliter volumes coupled with easily-implemented detection tools for probing target-drug interaction. We firstly show up a screening plat…
Luminometric Sub-nanoliter Droplet-to-droplet Arrays (LUMDA-chip) for Drug Screening on Phase I Metabolism Enzymes
2013
Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for amultiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. themost relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.
Micro and Nano patterns for Biosensing: from enzymatic assays to single cells interaction arrays
2012
In this thesis work, solution dispensing techniques have been employed for the realization of complex biological arrays. Inkjet printing techniques were employed for the generation of drug screening platforms. This approach was initially proved with a model enzyme system like Glucose Oxidase substrate covalently linked to a functionalized silicon oxide support. On this support an enzymatic substrate (D-glucose)/inhibitor (D-glucal) couple was accurately dispensed. A simple optical detection method was used to prove the screening capability of the microarray with the possibility to assay with high reproducibility at the single spot level. Afterwards, this methodology has been extended to CYP…
An Intra-Subject Approach Based on the Application of HMM to Predict Concentration in Educational Contexts from Nonintrusive Physiological Signals in…
2021
Previous research has proven the strong influence of emotions on student engagement and motivation. Therefore, emotion recognition is becoming very relevant in educational scenarios, but there is no standard method for predicting students’ affects. However, physiological signals have been widely used in educational contexts. Some physiological signals have shown a high accuracy in detecting emotions because they reflect spontaneous affect-related information, which is fresh and does not require additional control or interpretation. Most proposed works use measuring equipment for which applicability in real-world scenarios is limited because of its high cost and intrusiveness. To tackle this…
Structure Monitoring with BIM and IoT: The Case Study of a Bridge Beam Model
2022
The diffusion of Building Information Modelling (BIM) as a reference methodology, applied to the world of construction, leads to important changes in the design and the management of big constructions and infrastructures. However, although the BIM approach is necessary for managing the entire life cycle of a construction, today, this methodology is still rarely adopted beyond the design phase. This represents a major flaw because the management of every phase of the life cycle of buildings needs accurate preliminary planning. Certainly, one of the most complex and important phases of the life cycle of a construction is the monitoring phase, which represents a fundamental aspect for the main…
Radiation effects on optical frequency domain reflectometry fiber-based sensor
2015
International audience; We investigate the radiation effects on germanosilicate optical fiber acting as the sensing element of optical frequency domain reflectometry devices. Thanks to a new setup permitting to control temperature during irradiation, we evaluate the changes induced by 10 keV x rays on their Rayleigh response up to 1 MGy in a temperature range from −40°C up to 75°C. Irradiation at fixed temperature points out that its measure is reliable during both irradiation and the recovery process. Mixed temperature and radiation measurements show that changing irradiation temperature leads to an error in distributed measurements that depends on the calibration procedure. These results …
STUDIO E VALIDAZIONE DI SISTEMI PER L’IDENTIFICAZIONE E IL MONITORAGGIO DI PARAMETRI BIOMEDICI ATTI ALLA PREVENZIONE DI EVENTI INDESIDERATI E ALL’ASS…
L’aumento delle patologie cronico-degenerative nella popolazione mondiale ha comportato un numero sempre maggiore di individui richiedenti assistenza sanitaria, generando, nell’ambito delle realtà ospedaliere, diverse problematiche relative al miglioramento della qualità di vita dei pazienti, ai costi e alla gestione di un numero sempre crescente di soggetti ammalati. Il trattamento sanitario e la ricerca di base si sono pertanto orientati verso la prevenzione delle condizioni che predispongono i soggetti ai peggioramenti clinici e ai ricoveri ospedalieri, puntando sia su nuovi sistemi indossabili di monitoraggio per individuare precocemente i segni di peggioramento, sia su sistemi che poss…
Plasmonic Nanosensors for the Label-Free Imaging of Dynamic Protein Patterns
2020
Additional data to support our work on "Plasmonic Nanosensors for the Label-Free Imaging of Dynamic Protein Patterns" published in the Journal of Physical Chemistry Letters (DOI: 10.1021/acs.jpclett.0c01400) Movies: - S1: MinVideo_EColi.mp4 - S2: MinVideo_DOPC_DOPG_CL.mp4 - S3: MinVideo_DOPC_DOPG.mp4 Audio Files: - S1: MinSound_EColi.mp4 - S2: MinSound_DOPC_DOPG_CL.mp4 - S3: MinSound_DOPC_DOPG.mp4