Search results for "SEPARATION"
showing 10 items of 865 documents
PECAM-1 expression in human mesothelial cells: an in vitro study.
1996
Mesothelial cells are actively involved in inflammatory processes by expressing a set of cell adhesion molecules (CAMs). Transmigration of leukocytes into inflamed tissues requires a chemotactic stimulus and engagement of platelet-endothelial cell adhesion molecule-1 (PECAM-1). To investigate the kinetics involved in peritonitis, pure cultures of mesothelial cells are necessary. In previous studies, we have found that human mesothelial cells (HOMES) show a weak constitutive expression of PECAM-1, which cannot be further stimulated by cytokines. It is known that all serous cavities and body fluids contain numerous macrophages which strongly express this adhesion molecule. To identify the cel…
Binding properties of mechanically and enzymatically isolated hepatocytes for IgG and C3.
2008
— The presence of Fc and C3 receptors was studied on mechanically and enzymatically isolated rabbit, mouse and rat hepatocytes as well as on hepatocytes derived from primary cultures. The same cell preparations were used for studying the uptake of an antibody against nuclear antigens. Mechanically isolated hepatocytes were able to bind AIgG and activate complement. However, the same cells were not able to form rosettes with EA or with EAC. Enzymatically isolated hepatocytes did not bind AIgG or activate complement nor did they form rosettes with EA or with EAC. The mechanically isolated cells and the nonviable fraction of the enzymatically isolated cells showed a nuclear fluorescence when i…
Improved yield and functionality of parathyroid cells separated by using collagenase-digestion with cold pre-incubation.
2001
Preparation of cells from solid organs often induces a functional impairment due to the proteolytic cell damage by the applied digestive enzyme like collagenase, trypsin or dispase. To preserve the tissue and to enhance the yield of cells, Laue et al. reported an islet cell isolation with pre-incubation at 4 C permitting the enzyme to diffuse into the tissue and explicite activity equally throughout the whole particle. The aim of this study was to investigate whether this procedure can be applied to parathyroid glands. Therefore porcine parathyroid glands were dissected into 1 mm3 pieces. Subsequently one group of these pieces was incubated 22 h at 4 C in 2 mg/ml collagenase before activati…
Procedure to consistently obtain endothelial and smooth muscle cell cultures from umbilical cord vessels
2006
The prenatal history of an individual can be responsible to some extent for the occurrence of several diseases later in life. Thus, low birth weight has been related to an increased risk of developing hypertension or type 2 diabetes. The molecular and cellular basis of this increased risk could be found in body fluids and cell types that can be obtained just after birth. To get this unique information, a methodology was developed to consistently obtain cultures of 4 cell types, endothelial and smooth muscle cells from both the vein and the arteries present in the umbilical cord of an individual. From 21 umbilical cords processed, 82 of the 84 possible cell cultures were obtained. The cell c…
Isolation and characterization of a murine resident liver stem cell.
2008
Increasing evidence provides support that mammalian liver contains stem/progenitor cells, but their molecular phenotype, embryological derivation, biology and their role in liver cell turnover and regeneration remain to be further clarified. In this study, we report the isolation, characterization and reproducible establishment in line of a resident liver stem cell (RLSC) with immunophenotype and differentiative potentiality distinct from other previously described liver precursor/stem cells. RLSCs, derived from fetal and neonatal murine livers as well as from immortalized hepatocytic MMH lines and established in lines, are Sca+, CD34-, CD45-, alpha-fetoprotein+ and albumin-. This molecular…
Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacity.
2008
Colon carcinoma is one of the leading causes of death from cancer and is characterized by a heterogenic pool of cells with distinct differentiation patterns. Recently, it was reported that a population of undifferentiated cells from a primary tumor, so-called cancer stem cells (CSC), can reconstitute the original tumor on xenotransplantation. Here, we show that spheroid cultures of these colon CSCs contain expression of CD133, CD166, CD44, CD29, CD24, Lgr5, and nuclear β-catenin, which have all been suggested to mark the (cancer) stem cell population. More importantly, by using these spheroid cultures or freshly isolated tumor cells from multiple colon carcinomas, we now provide compelling…
Extramedullary Expansion of Hematopoietic Progenitor Cells in Interleukin (IL)-6–sIL-6R Double Transgenic Mice
1997
Soluble cytokine receptors modulate the activity of their cognate ligands. Interleukin (IL)-6 in association with the soluble IL-6 receptor (sIL-6R) can activate cells expressing the gp130 signal transducer lacking the specific IL-6R. To investigate the function of the IL-6–sIL-6R complex in vivo and to discriminate the function of the IL-6–sIL-6R complex from the function of IL-6 alone, we have established a transgenic mouse model. Double-transgenic mice coexpressing IL-6 and sIL-6R were generated and compared with IL-6 and sIL-6R single-transgenic mice. The main phenotype found in IL-6–sIL-6R mice was a dramatic increase of extramedullary hematopoietic progenitor cells in liver and spleen…
Negative-pressure cavitation coupled with aqueous two-phase extraction and enrichment of flavonoids and stilbenes from the pigeon pea leaves and the …
2015
Abstract An efficient extraction method, negative-pressure cavitation coupled with aqueous two-phase extraction (NPC-ATPE), was developed to simultaneously extract and enrich flavonoids and stilbenes from pigeon pea leaves. The main bioactive compounds, cajaninstilbene acid (CSA), longistyline C (LLC), pinostrobin (PI) and pinostrobin chalcone (PIC) were chosen as target compounds. The extraction conditions were optimized using central composite design combined with response surface methodology. The optimized conditions were: concentration of (NH 4 ) 2 SO 4 21% (w/w), concentration of ethanol 23% (w/w) and temperature 30 °C, negative pressure −0.06 MPa, extraction time 30 min and the liquid…
ChemInform Abstract: Bubble Fractionation of Enantiomers from Solution Using Molecularly Imprinted Polymers as Collectors.
2010
Adsorptive bubble separation methods have been used to enrich components from both heterogeneous and homogeneous solutions. These methods are particularly effective for processing large solution volumes at low cost. Previous work demonstrated that chiral, surface-active collectors could be used to enrich enantiomers from homogeneous solution in a foam fractionation process. In a significant extension of this work, the use of highly selective molecularly imprinted polymers (MIPs) and heterogeneous solutions for the bubble flotation of enantiomers was evaluated. The high selectivity and ease of recycling of the MIP make this a potentially powerful approach for process-scale separations from l…
Direct identification of 103–107Mo by a rapid chemical separation procedure
1977
Abstract The nuclides 103–107Mo have been identified by means of γ-ray spectroscopy after the separation of molybdenum from fission products of 235U and 239Pu by a rapid chemical procedure. Half-lives of 67.5 ± 1.5 s for 103Mo, 59.4 ± 0.8 s for 104Mo, 36.7 ± 1.0 s for 105Mo, 8.4 ± 0.5 s for 106Mo and 3.5 ± 0.5 s for 107Mo have been determined and the main γ-lines were assigned to the decay of these isotopes. Some evidence for 108Mo with a half-life ≤2 s has also been obtained.