Search results for "SOI"
showing 10 items of 4823 documents
Fingerprinting and diversity of bacterial copA genes in response to soil types, soil organic status and copper contamination
2007
A molecular fingerprinting assay was developed to assess the diversity of copA genes, one of the genetic determinants involved in bacterial resistance to copper. Consensus primers of the copA genes were deduced from an alignment of sequences from proteobacterial strains. A PCR detection procedure was optimized for bacterial strains and allowed the description of a novel copA genetic determinant in Pseudomonas fluorescens. The copA DNA fingerprinting procedure was optimized for DNA directly extracted from soils differing in their physico-chemical characteristics and in their organic status (SOS). Particular copA genetic structures were obtained for each studied soil and a coinertia analysis …
Quantitative Detection of the nosZ Gene, Encoding Nitrous Oxide Reductase, and Comparison of the Abundances of 16S rRNA, narG , nirK , and nosZ Genes…
2006
ABSTRACT Nitrous oxide (N 2 O) is an important greenhouse gas in the troposphere controlling ozone concentration in the stratosphere through nitric oxide production. In order to quantify bacteria capable of N 2 O reduction, we developed a SYBR green quantitative real-time PCR assay targeting the nosZ gene encoding the catalytic subunit of the nitrous oxide reductase. Two independent sets of nosZ primers flanking the nosZ fragment previously used in diversity studies were designed and tested (K. Kloos, A. Mergel, C. Rösch, and H. Bothe, Aust. J. Plant Physiol. 28:991-998, 2001). The utility of these real-time PCR assays was demonstrated by quantifying the nosZ gene present in six different …
Phylogeny of viroids, viroidlike satellite RNAs, and the viroidlike domain of hepatitis delta virus RNA.
1991
We report a phylogenetic study of viroids, some plant satellite RNAs, and the viroidlike domain of human hepatitis delta virus RNA. Our results support a monophyletic origin of these RNAs and are consistent with the hypothesis that they may be "living fossils" of a precellular RNA world. Moreover, the viroidlike domain of human hepatitis delta virus RNA appears closely related to the viroidlike satellite RNAs of plants, with which it shares some structural and functional properties. On the basis of our phylogenetic analysis, we propose a taxonomic classification of these RNAs.
Compatibility of Rhizobial Genotypes within Natural Populations of Rhizobium leguminosarum Biovar viciae for Nodulation of Host Legumes
2003
ABSTRACT Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean ( Vicia faba ) and pea ( Pisum sativum ) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches ( Vicia sativa ) grown in other soils and fava bean-nodulating strains from various geographic sites were also analyzed. The rhizobia were characterized by repetitive extragenomic palindromic-PCR fingerprinting and/or PCR-restriction fragment length polymorphism (RFLP) of 16S-23S ribosomal DNA intergenic spacers as markers of the genomic background and PCR-RFLP of a nodulation gene region, nodD , as a marker of the sy…
Contamination Remediation with Soil Amendments by Immobilization of Heavy Metals
2015
Elektroniskā versija nesatur pielikumus
Isotope fractionation of zinc in the paddy rice soil-water environment and the role of 2’deoxymugineic acid (DMA) as zincophore under Zn limiting con…
2021
Non-traditional stable isotope systems are increasingly used to study micronutrient cycling and acquisition in terrestrial ecosystems. We previously proposed for zinc (Zn) a conceptual model linking observed isotope signatures and fractionations to biogeochemical processes occurring in the rice soil environment and we suggested that 2’deoxymugineic acid (DMA) could play an important role for rice during the acquisition of Zn when grown under Zn limiting conditions. This proposition was sustained by the extent and direction of isotope fractionation observed during the complexation of Zn with DMA synthesised in our laboratory. Here we report a new set of experimental data from field and labor…
Geogenic element behaviour in soil-rainwater interaction at Mt Etna, Sicily: preliminary results
2008
Active volcanoes emit considerable amounts of contaminants such as As, Se and V. Previous studies have shown that the volcanic activity at Mt Etna (Sicily) has a strong influence on local rainwater compositions. However to date, the behaviour of trace elements in the soils around Mt Etna is poorly understood. 4-hr batch experiments have been performed with 1:5 soil solutions of air-dried soil (fraction <2 mm) and synthetic (acid) rainwater (using either deionized water with a pH of ~6 or a ~500 ppm of sulphuric acid solution with a pH of ~2). In general trace element concentrations are more enriched in soil solutions with low pH (e.g. enrichment factor (EF) acid compared to neutral soil sol…
Spatial prediction of soil erosion susceptibility using a fuzzy analytical network process: Application of the fuzzy decision making trial and evalua…
2018
Soil erosion is a worldwide threat that results in soil degradation, agriculture abandonment, and crop yield reduction. There is a need to find methods to survey soil erosion rates in order to improve and develop sustainable land planning. The present study utilizes new approaches based on the fuzzy set both in designing the problem (through the fuzzy decision making trial and evaluation laboratory) and in prioritizing the effective factors to mitigate soil erosion (using a fuzzy analytical network process, FANP). This study is first to apply these methods to soil erosion. A set of geo‐environmental factors influencing soil erosion was characterized to evaluate the potential risk of soil er…
Ecological risks of an old wood impregnation mill: application of the triad approach.
2010
Although many studies deal with the distribution and mobility of chromated copper arsenate (CCA) metals in soil, the ecotoxicity of CCA-contaminated soils is rarely studied. The Triad approach was applied to determine the ecological risks posed by a CCA mixture at a decommissioned wood impregnation mill in southern Finland. A combination of (1) chemical analyses; (2) toxicity tests with plants (aquatic: Lemna minor; terrestrial: Lactuca sativa), earthworms (Lumbricus rubellus), and enchytraeids (Enchytraeus albidus) conducted on contaminated soils, their aqueous extracts, and well water collected from the site; and (3) determination of the abundance of enchytraeids and nematodes and the bio…
Reconfigurations in sustainability transitions : a systematic and critical review
2021
Two streams of literature have become especially prominent in understanding social change toward sustainability within the past decades: the research on socio-technical transitions and applications of social practice theory. The aim of this article is to contribute to efforts to create dialogue between these two approaches. We do this by focusing on the concept of reconfiguration, which has become a much-used, but poorly defined notion in the discussion on sustainability transitions. To understand what is defined as reconfiguration in systems and practices, and how the understanding of reconfiguration in regimes could benefit from insights about reconfiguration in practices, we conducted a …