Search results for "STRAIN"
showing 10 items of 1955 documents
Differential expression of mRNA encoding interleukin-12 p35 and p40 subunitsin situ
1994
Interleukin-12 (IL-12) is a heterodimeric cytokine that plays an important role in the regulation of the immune response. For biological activity the expression of both subunits of IL-12, p35 and p40, is required. Moreover, in the mouse the p40 chain of IL-12 specifically inhibits the effects of the IL-12 heterodimer. In the present study we have analyzed by in situ hybridization the expression of the p35 and p40 mRNA in the spleens of BALB/c and mutant (SCID, nude, beige) mice, unstimulated and after in vivo stimulation with lipopolysaccharide (LPS) and with staphylococcal enterotoxin B (SEB). In unstimulated spleens of BALB/c mice p35 and p40 mRNA were only detectable in a few strongly st…
Selected enzyme activities in mouse cardiac muscle during training and terminated training
1984
We studied the effects of running-training, heavy exercise and termination of training on the heart weight, the ratio heart to body weight and the cardiac muscle activities of actomyosin ATPase, citrate synthase, succinate dehydrogenase, cytochrome c oxidase, malate dehydrogenase, adenylate kinase and beta-glucuronidase with adult male NMRI-mice. Stable hypertrophy (6-7%), estimated by the ratio heart or ventricle weight to body weight, was achieved by 28 exercises and it was dependent on the running speed (20 vs. 25 m X min-1). The withdrawal of training for 5-61 days did not permanently decrease the heart weight or the heart to body weight ratio to the level of sedentary controls. The act…
Search for Dark Matter Annihilations in the Sun with the 79-String IceCube Detector
2012
We have performed a search for muon neutrinos from dark matter annihilation in the center of the Sun with the 79-string configuration of the IceCube neutrino telescope. For the first time, the DeepCore sub-array is included in the analysis, lowering the energy threshold and extending the search to the austral summer. The 317 days of data collected between June 2010 and May 2011 are consistent with the expected background from atmospheric muons and neutrinos. Upper limits are therefore set on the dark matter annihilation rate, with conversions to limits on spin-dependent and spin-independent WIMP-proton cross-sections for WIMP masses in the range 20 - 5000 GeV. These are the most stringent s…
"Synaptic" ribbons and spherules of the rat pineal gland: day/night changes in vitro?
1982
In the present study pineal glands of rats aged 69–71 days were studied in vivo and in vitro with respect to day/night changes of “synaptic” ribbons and spherules. It was found that ribbons outnumber spherules by a factor of 3. In vivo, both ribbons and spherules show a roughly 3-fold increase in number at 1 a.m. when compared to 1 p.m. Up to 39 h in vitro, the two structures in question did not reveal day/night differences in amount, suggesting that diurnal rhythmicity of the gland did apparently not persist in organ culture. After 3 h in organ culture, the spherules, but not the ribbons, showed a striking increase in number, showing that ribbons and spherules may be governed by different …
Fully covariant and conformal formulation of the Z4 system in a reference-metric approach: Comparison with the BSSN formulation in spherical symmetry
2014
We adopt a reference-metric approach to generalize a covariant and conformal version of the Z4 system of the Einstein equations. We refer to the resulting system as ``fully covariant and conformal", or fCCZ4 for short, since it is well suited for curvilinear as well as Cartesian coordinates. We implement this fCCZ4 formalism in spherical polar coordinates under the assumption of spherical symmetry using a partially-implicit Runge-Kutta (PIRK) method and show that our code can evolve both vacuum and non-vacuum spacetimes without encountering instabilities. Our method does not require regularization of the equations to handle coordinate singularities, nor does it depend on constraint-preservi…
Sympathetic Nerve Stimulation on the perfused rat heart. Affinities of N-methylatropine and pirenzepine at pre- and postsynaptic muscarine receptors.
1982
Rat isolated hearts with the sympathetic nerves attached were perfused with (-)-3H-noradrenaline in order to label the storage vesicles of the adrenergic nerves. Release was induced either by electrical stimulation of the nerves (3 Hz, 1 min) or by perfusion with high K+ solution (54 mM). The overflow of 3H-noradrenaline and its metabolites was determined by liquid scintillation counting after separation of the compounds by column chromatography. The experimental conditions ensured a minor contribution of 3H-metabolites to the evoked total tritium overflow. The release of 3H-noradrenaline evoked by nerve stimulation or high K+ solution was decreased in the presence of the muscarinic agonist…
The linear saturated decentralized strategy for constrained flow control is asymptotically optimal
2013
We present an algorithm for constrained network flow control in the presence of an unknown demand. Our algorithm is decentralized in the sense that it is implemented by a team of agents, each controlling just the flow on a single arc of the network based only on the buffer levels at the nodes at the extremes of the arc, while ignoring the actions of other agents and the network topology. We prove that our algorithm is also stabilizing and steady-state optimal. Specifically, we show that it asymptotically produces the minimum-norm flow. We finally generalize our algorithm to networks with a linear dynamics and we prove that certain least-square optimality properties still hold.
Creating a conditional mutation of Wnt-1 by antisense transgenesis provides evidence that Wnt-1 is not essential for spermatogenesis.
1993
We have used mice transgenic for an antisense construct for Wnt-1 to study the role of this gene in post-meiotic sperm development. The human PGK-2 promoter provided levels of Wnt-1 antisense mRNA in testes in 5 transgenic lines greatly in excess of Wnt-1 mRNA concentrations, and Wnt-1 mRNA levels were greatly decreased in the lines, by 98% in three of them. There was a general correlation between copy number of the insert, levels of antisense RNA, and decreases in mRNA. There was little effect of the antisense transgene on fertility or testicular histology suggesting that normal levels of Wnt-1 transcript are not essential for spermatogenesis.
Three-dimensional matching based resource provisioning for the design of low-latency heterogeneous IoT networks
2019
Internet-of-Things (IoT) is a networking architecture where promising, intelligent services are designed via leveraging information from multiple heterogeneous sources of data within the network. However, the availability of such information in a timely manner requires processing and communication of raw data collected from these sources. Therefore, the economic feasibility of IoT-enabled networks relies on the efficient allocation of both computational and communication resources within the network. Since fog computing and 5G cellular networks approach this problem independently, there is a need for joint resource-provisioning of both communication and computational resources in the networ…
Expression of human estrogen sulfotransferase in Salmonella typhimurium: differences between hHST and hEST in the enantioselective activation of 1-hy…
1998
Various human sulfotransferases (hP-PST, hM-PST, hHST) and rat sulfotransferases (rPST-IV, rHSTa) have already been expressed in Ames' Salmonella strains (in particular in TA1538). Now a further strain, TA1538-hEST, which expresses the human estrogen sulfotransferase (hEST), has been constructed. This strain activated the primary benzylic alcohol 1-hydroxymethylpyrene (1-HMP) and the secondary benzylic alcohol 1-hydroxyethylpyrene (1-HEP) to mutagens. Human sulfotransferases hEST and hHST both activated 1-HEP, but they differed substantially in their enantioselectivity for this compound.