Search results for "STRESS"

showing 10 items of 6278 documents

A Drosophila model of GDAP1 function reveals the involvement of insulin signalling in the mitochondria-dependent neuromuscular degeneration

2017

[EN] Charcot-Marie-Tooth disease is a rare peripheral neuropathy for which there is no specific treatment. Some forms of Charcot-Marie-Tooth are due to mutations in the GDAP1 gene. A striking feature of mutations in GDAP1 is that they have a variable clinical manifestation, according to disease onset and progression, histology and mode of inheritance. Studies in cellular and animal models have revealed a role of GDAP1 in mitochondrial morphology and distribution, calcium homeostasis and oxidative stress. To get a better understanding of the disease mechanism we have generated models of over-expression and RNA interference of the Drosophila Gdapl gene. In order to get an overview about the c…

0301 basic medicineCharcot-Marie-Toothmedicine.medical_treatmentNerve Tissue ProteinsGDAP1MitochondrionBiologymedicine.disease_cause03 medical and health sciencesCharcot-Marie-Tooth DiseaseRNA interferenceGene expressionBIOQUIMICA Y BIOLOGIA MOLECULARmedicineAnimalsDrosophila ProteinsHumansInsulinMolecular BiologyGeneticsMechanism (biology)InsulinNeurodegenerationLipid Metabolismmedicine.diseaseUp-RegulationMitochondriaCell biology030104 developmental biologyMetabolomeCarbohydrate MetabolismMolecular MedicineDrosophilaRNA InterferenceOxidative stressFunction (biology)Signal TransductionBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
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Bevacizumab diminishes inflammation in an acute endotoxin-induced uveitis model

2017

Este artículo se encuentra disponible en la página web de la revista en la siguiente URL: https://www.frontiersin.org/articles/10.3389/fphar.2018.00649/full Introduction: Uveitis is an eye disease characterized by inflammation of the uvea and an early and exhaustive diagnosis is essential for its treatment. The aim of our study is to assess the potential toxicity and anti-inflammatory efficacy of Bevacizumab in an experimental uveitis model by subcutaneously injecting lipopolysaccharide into Lewis rats and to clarify its mechanism. Material and Methods: Blood–aqueous barrier integrity was assessed 24 h after endotoxin-induced uveitis (EIU) by analyzing two parameters: cell count and protein…

0301 basic medicineChemokineLipopolysaccharidegenetic structuresmedicine.medical_treatmentÚvea - Efectos de los medicamentos.chemokinesPharmacologymedicine.disease_causeendotoxin-induced uveitischemistry.chemical_compound0302 clinical medicineMedicineoxidative stressPharmacology (medical)Bevacizumab - Efectos fisiológicos.Bevacizumab - Efectos secundarios.Uvea - Effect of drugs on.Original ResearchEstrés oxidativo.biologyOxidative stress.medicine.anatomical_structureCytokineToxicityOjos - Enfermedades - Tratamiento.medicine.symptomUveitisPharmacology.InflammationFarmacología.bevacizumabBevacizumab - Physiological effect.Bevacizumab - Side effects.03 medical and health sciencesUveitis - Treatment.Eyes - Diseases - Treatment.Pharmacologybusiness.industrylcsh:RM1-950Uveítis - Tratamiento.Uveamedicine.diseaseeye diseasescytokines030104 developmental biologylcsh:Therapeutics. Pharmacologychemistryinflammation030221 ophthalmology & optometrybiology.proteinsense organsbusinessOxidative stress
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Chronic stress leads to epigenetic dysregulation in the neuropeptide-Y and cannabinoid CB1 receptor genes in the mouse cingulate cortex.

2017

Persistent stress triggers a variety of mechanisms, which may ultimately lead to the occurrence of anxiety- and depression-related disorders. Epigenetic modifications represent a mechanism by which chronic stress mediates long-term effects. Here, we analyzed brain tissue from mice exposed to chronic unpredictable stress (CUS), which induced impaired emotional and nociceptive behaviors. As endocannabinoid (eCB) and neuropeptide-Y (Npy) systems modulate emotional processes, we hypothesized that CUS may affect these systems through epigenetic mechanisms. We found reduced Npy expression and Npy type 1 receptor (Npy1r) signaling, and decreased expression of the cannabinoid type 1 receptor (CB1) …

0301 basic medicineCingulate cortexMalemedicine.medical_specialtyCannabinoid receptormedicine.medical_treatmentBiologyGyrus CinguliEpigenesis Genetic03 medical and health sciencesCellular and Molecular Neurosciencechemistry.chemical_compoundMice0302 clinical medicineReceptor Cannabinoid CB1Internal medicinemental disordersmedicineAnimalsHumansChronic stressNeuropeptide YPharmacologyHistone deacetylase 2URB597Endocannabinoid systemhumanitiesMice Inbred C57BL030104 developmental biologyEndocrinologychemistryBenzamidesCannabinoidHistone deacetylaseCarbamates030217 neurology & neurosurgeryStress PsychologicalNeuropharmacology
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Dual proteotoxic stress accelerates liver injury via activation of p62‐Nrf2

2021

Protein accumulation is the hallmark of various neuronal, muscular, and other human disorders. It is also often seen in the liver as a major protein-secretory organ. For example, aggregation of mutated alpha1-antitrypsin (AAT), referred to as PiZ, is a characteristic feature of AAT deficiency, whereas retention of hepatitis B surface protein (HBs) is found in chronic hepatitis B (CHB) infection. We investigated the interaction of both proteotoxic stresses in humans and mice. Animals overexpressing both PiZ and HBs (HBs-PiZ mice) had greater liver injury, steatosis, and fibrosis. Later they exhibited higher hepatocellular carcinoma load and a more aggressive tumor subtype. Although PiZ and H…

0301 basic medicineCirrhosisNF-E2-Related Factor 2medicine.disease_causePathology and Forensic MedicineMice03 medical and health sciences0302 clinical medicineSDG 3 - Good Health and Well-beingStress PhysiologicalFibrosisSequestosome-1 ProteinmedicineAnimalsHumansLiver injuryHepatitis B Surface Antigensbusiness.industryLiver DiseasesAutophagyHepatitis Bmedicine.diseasedigestive system diseasesaggregate Hepatitis B Surface Antigens Humans Liver Diseases Mice NF-E2-Related Factor 2 Sequestosome-1 Protein Stress Physiological alpha 1-Antitrypsin cirrhosis inclusionlipophagy oxidative stress SERPINA1 Animals030104 developmental biologyalpha 1-Antitrypsin030220 oncology & carcinogenesisHepatocellular carcinomaCancer researchSteatosisbusinessOxidative stressThe Journal of Pathology
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Impact of high-pressure processing on the stability and bioaccessibility of bioactive compounds in Clementine mandarin juice and its cytoprotective e…

2020

Mandarin juice is a rich source of antioxidant bioactive compounds. While the content and profile of bioactives are known, the impact of high-pressure processing (HPP) on their stability and bioaccessibility (BA) is unknown, but may allow obtaining safe, nutritious, and fresh-tasting juices with highly extractable bioactive compounds. The stability and BA of bioactive antioxidant compounds in untreated and HPP-treated (400 MPa/40 °C/1 min) Clementine mandarin juices, and the cytoprotective effect of its bioaccessible fractions (BF) obtained after simulated gastrointestinal digestion against H2O2-induced oxidative stress in differentiated Caco-2 cells were investigated. The BF of HPP-treated…

0301 basic medicineCitrusAntioxidantFood Handlingmedicine.medical_treatmentPhytochemicalsBiological AvailabilityPascalization03 medical and health sciences0404 agricultural biotechnologyCryoprotective AgentsAntioxidant activitymedicineHumansBeta-cryptoxanthinFood scienceOrange juiceCarotenoidchemistry.chemical_classificationOrange juicePulsed electric-fields030109 nutrition & dieteticsVitamin CVitamin-c04 agricultural and veterinary sciencesGeneral MedicineAscorbic acid040401 food scienceCytoprotectionIn-vitro bioaccessibilityFlavonoid compositionFruit and Vegetable JuiceschemistryPolyphenolOxidative stressCitrus juiceAscorbic acidCaco-2 CellsFood ScienceFoodfunction
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The potential of aldehyde dehydrogenase 2 as a therapeutic target in cardiovascular disease.

2018

Mitochondrial aldehyde dehydrogenase (ALDH-2) plays a major role in the ethanol detoxification pathway by removing acetaldehyde. Therefore, ALDH-2 inhibitors such as disulfiram represent the first therapeutic targeting of ALDH-2 for alcoholism therapy. Areas covered: Recently, ALDH-2 was identified as an essential bioactivating enzyme of the anti-ischemic organic nitrate nitroglycerin, bringing ALDH-2 again into the focus of clinical interest. Mechanistic studies on the nitroglycerin bioactivation process revealed that during bioconversion of nitroglycerin and in the presence of reactive oxygen and nitrogen species the active site thiols of ALDH-2 are oxidized and the enzyme activity is los…

0301 basic medicineClinical BiochemistryAldehyde dehydrogenasemedicine.disease_causeAntioxidants03 medical and health scienceschemistry.chemical_compoundDetoxificationDrug DiscoverymedicineAnimalsHumansMolecular Targeted TherapyPharmacologyEthanolbiologyAldehyde Dehydrogenase MitochondrialMitochondrial Aldehyde DehydrogenaseAcetaldehydeCardiovascular AgentsDisease Models AnimalOxidative Stress030104 developmental biologyBiochemistrychemistryCardiovascular DiseasesDrug DesignCardiovascular agentDisulfirambiology.proteinMolecular MedicineOxidative stressmedicine.drugExpert opinion on therapeutic targets
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Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidati…

2019

Oxidative stress and a disturbed cellular protein homeostasis (proteostasis) belong to the most important hallmarks of aging and of neurodegenerative disorders. The proteasomal and autophagic-lysosomal degradation pathways are key measures to maintain proteostasis. Here, we report that hippocampal cells selected for full adaptation and resistance to oxidative stress induced by hydrogen peroxide (oxidative stress-resistant cells, OxSR cells) showed a massive increase in the expression of components of the cellular autophagic-lysosomal network and a significantly higher overall autophagic activity. A comparative expression analysis revealed that distinct key regulators of autophagy are upregu…

0301 basic medicineClinical BiochemistryLFQ Label-free quantificationLETM Leucine zipper and EF-hand containing transmembrane proteinmedicine.disease_causeBiochemistryCHX Cycloheximide0302 clinical medicineBNIP3 Bcl-2 interacting protein 3RAPA RapamycinPIK3C3 Class III PI3‐kinasePhosphorylationlcsh:QH301-705.5Neuronslcsh:R5-920PolyUB PolyubiquitinChemistryBAG3OPA1 Optic atrophy 1TOR Serine-Threonine KinasesWIPI1 WD repeat domain phosphoinositide-interacting protein 1ATG Autophagy relatedTFEB Transcription factor EBCell biologyMitochondriasiRNA Small interfering RNADLP1 Dynamin-like protein 1LAMP1 Lysosomal‐associated membrane protein 1PURO Puromycinlcsh:Medicine (General)Protein homeostasisResearch PaperBafA1 Bafilomycin A1LAMP2 Lysosomal‐associated membrane protein 2Proteasome Endopeptidase ComplexRAB18 Member RAS oncogeneTUB TubulinLC3 Light chain 3 proteinOxidative phosphorylationBAG3CTSD Cathepsin DModels BiologicalCell Line03 medical and health sciencesDownregulation and upregulationMacroautophagymedicineAutophagyHumansAdaptationBAG1 Bcl-2-associated athanogene 1BECN1 Beclin1PI3K/AKT/mTOR pathwayAdaptor Proteins Signal TransducingTEM Transmission electron microscopyHsp70 Heat shock protein 70Organic ChemistryAutophagyAutophagosomesmTOR Mammalian target of rapamycinHsp70Oxidative Stress030104 developmental biologyProteostasislcsh:Biology (General)CV CanavanineBAG3 Bcl-2-associated athanogene 3MTT (3-(45-Dimethylthiazol-2-yl)-25-Diphenyltetrazolium Bromide)Apoptosis Regulatory ProteinsLysosomes030217 neurology & neurosurgeryOxidative stressRedox Biology
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Effects of gaseous and solid constituents of air pollution on endothelial function

2018

Abstract Ambient air pollution is a leading cause of non-communicable disease globally. The largest proportion of deaths and morbidity due to air pollution is now known to be due to cardiovascular disorders. Several particulate and gaseous air pollutants can trigger acute events (e.g. myocardial infarction, stroke, heart failure). While the mechanisms by which air pollutants cause cardiovascular events is undergoing continual refinement, the preponderant evidence support rapid effects of a diversity of pollutants including all particulate pollutants (e.g. course, fine, ultrafine particles) and gaseous pollutants such as ozone, on vascular function. Indeed alterations in endothelial function…

0301 basic medicineClinical ReviewFine particulateAir pollutionAir pollution030204 cardiovascular system & hematologymedicine.disease_cause03 medical and health sciencesGreenhouse Gases0302 clinical medicineOzoneRisk FactorsEnvironmental healthMedicineAnimalsHumansEndothelial dysfunctionNitrogen dioxidePollutantInflammationAir PollutantsHuman studiesAmbient air pollutionbusiness.industryEnvironmental ExposureParticulates3. Good healthEditor's Choice030104 developmental biology13. Climate actionCardiovascular DiseasesOxidative stressEndothelium VascularCurrent (fluid)Cardiology and Cardiovascular MedicinebusinessVascular functionParticulate matterTranslational MedicineEuropean Heart Journal
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Gut microbiota imbalance and colorectal cancer

2016

International audience; The gut microbiota acts as a real organ. The symbiotic interactions between resident micro-organisms and the digestive tract highly contribute to maintain the gut homeostasis. However, alterations to the microbiome caused by environmental changes (e.g., infection, diet and/or lifestyle) can disturb this symbiotic relationship and promote disease, such as inflammatory bowel diseases and cancer. Colorectal cancer is a complex association of tumoral cells, non-neoplastic cells and a large amount of micro-organisms, and the involvement of the microbiota in colorectal carcinogenesis is becoming increasingly clear. Indeed, many changes in the bacterial composition of the g…

0301 basic medicineColorectal cancer[SDV]Life Sciences [q-bio]enterotoxigenic bacteroides-fragilisGut floraCyclomodulin[ SDV.CAN ] Life Sciences [q-bio]/CancerTopic Highlightstreptococcus-gallolyticus infectionbiologyGastrointestinal MicrobiomeGastroenterologyGeneral Medicinecytolethal-distending toxin3. Good healthlactobacillus-acidophilus deficientIntestinesCell Transformation NeoplasticHost-Pathogen InteractionsInflammation MediatorsColorectal NeoplasmsVirulence Factorspolymerase-chain-reaction[SDV.CAN]Life Sciences [q-bio]/CancerGut microbiotaoxidative dna-damageMicrobiologyescherichia-coli strains03 medical and health scienceshelicobacter-pylori infectionmedicineAnimalsHumansMicrobiomeBacteria[ SDV ] Life Sciences [q-bio]inflammatory-bowel-diseaseCancerHelicobacter pyloribiology.organism_classificationmedicine.diseaseStreptococcus bovisColorectal cancerGastrointestinal MicrobiomeHépatologie et Gastroentérologie030104 developmental biologytoll-like receptorsOxidative stressImmunologyHépatology and GastroenterologyDysbiosiscolorectal cancer;gut microbiota;dysbiosis;cyclomodulin;oxidative;stress;enterotoxigenic bacteroides-fragilis;oxidative dna-damage;cytolethal-distending toxin;inflammatory-bowel-disease;streptococcus-gallolyticus infection;lactobacillus-acidophilus;deficient;helicobacter-pylori infection;polymerase-chain-reaction;escherichia-coli strains;toll-like receptorsDysbiosisDNA Damage
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BIOMARKERS OF CORONARY MICROVASCULAR DYSFUNCTION IN PATIENTS WITH MICROVASCULAR ANGINA: A NARRATIVE REVIEW

2021

The current gold standard for diagnosis of coronary microvascular dysfunction (CMD) in the absence of myocardial diseases, whose clinical manifestation is microvascular angina (MVA), is reactivity testing using adenosine or acetylcholine during coronary angiography. This invasive test can be difficult to perform, expensive, and harmful. The identification of easily obtainable blood biomarkers which reflect the pathophysiology of CMD, characterized by high reliability, precision, accuracy, and accessibility may reduce risks and costs related to invasive procedures and even facilitate the screening and diagnosis of CMD. In this review, we summarized the results of several studies that have i…

0301 basic medicineCoronary angiographymedicine.medical_specialtyClinical manifestationCoronary Artery Disease030204 cardiovascular system & hematology03 medical and health sciences0302 clinical medicinemicrovascular dysfunction (CMD) oxidative stress inflammation endothelial dysfunction microvascular angina (MVA)Internal medicineCoronary CirculationMedicineHumansIn patientEndothelial dysfunctionMicrovascular Anginabusiness.industryMicrocirculationMicrovascular anginaReproducibility of ResultsGold standard (test)medicine.disease030104 developmental biologyCardiologyNarrative reviewCardiology and Cardiovascular MedicinebusinessBiomarkersReactivity Testing
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