Impact of high-pressure processing on the stability and bioaccessibility of bioactive compounds in Clementine mandarin juice and its cytoprotective effect on Caco-2 cells.

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RESEARCH PRODUCT

Impact of high-pressure processing on the stability and bioaccessibility of bioactive compounds in Clementine mandarin juice and its cytoprotective effect on Caco-2 cells.

M. Pilar Cano María Jesús Rodrigo Lorenzo Zacarías Concepción Sánchez-moreno Begoña De Ancos Reyes Barberá Antonio Cilla Amparo Alegría

subject

0301 basic medicine Citrus Antioxidant Food Handling medicine.medical_treatment Phytochemicals Biological Availability Pascalization 03 medical and health sciences 0404 agricultural biotechnology Cryoprotective Agents Antioxidant activity medicine Humans Beta-cryptoxanthin Food science Orange juice Carotenoid chemistry.chemical_classification Orange juice Pulsed electric-fields 030109 nutrition & dietetics Vitamin C Vitamin-c 04 agricultural and veterinary sciences General Medicine Ascorbic acid 040401 food science Cytoprotection In-vitro bioaccessibility Flavonoid composition Fruit and Vegetable Juices chemistry Polyphenol Oxidative stress Citrus juice Ascorbic acid Caco-2 Cells Food Science

description

Mandarin juice is a rich source of antioxidant bioactive compounds. While the content and profile of bioactives are known, the impact of high-pressure processing (HPP) on their stability and bioaccessibility (BA) is unknown, but may allow obtaining safe, nutritious, and fresh-tasting juices with highly extractable bioactive compounds. The stability and BA of bioactive antioxidant compounds in untreated and HPP-treated (400 MPa/40 °C/1 min) Clementine mandarin juices, and the cytoprotective effect of its bioaccessible fractions (BF) obtained after simulated gastrointestinal digestion against H2O2-induced oxidative stress in differentiated Caco-2 cells were investigated. The BF of HPP-treated juices showed a better retention of carotenoids, flavonoids, ascorbic acid, total polyphenols and FRAP value, and slightly higher cytoprotection (mitochondrial membrane potential and ROS) than untreated juices. Therefore, HPP can be recommended as a suitable technology to retain or indeed increase antioxidant bioactives and their cytoprotective activity in mandarin juices after gastrointestinal digestion.

year	journal	country	edition	language
2020-01-01	Foodfunction

10.1039/d0fo02048f https://pubmed.ncbi.nlm.nih.gov/33001074