Search results for "Sack"
showing 10 items of 55 documents
Infectious Entry Pathway of Enterovirus B Species
2015
Enterovirus B species (EV-B) are responsible for a vast number of mild and serious acute infections. They are also suspected of remaining in the body, where they cause persistent infections contributing to chronic diseases such as type I diabetes. Recent studies of the infectious entry pathway of these viruses revealed remarkable similarities, including non-clathrin entry of large endosomes originating from the plasma membrane invaginations. Many cellular factors regulating the efficient entry have recently been associated with macropinocytic uptake, such as Rac1, serine/threonine p21-activated kinase (Pak1), actin, Na/H exchanger, phospholipace C (PLC) and protein kinase Cα (PKCα). Another…
Enterovirus-induced non-acidic entry pathway and its relation to the epidermal growth factor receptor pathway
2014
Infectious Entry Pathway of Enterovirus B Species
2015
Unveiling adaptive mechanisms though experimental evolution: the role of duplicated genes and phenotypic plasticity in yeast, and the genetic variabi…
2021
Los seres vivos se enfrentan a condiciones ambientales cambiantes y habitualmente estresantes, agravadas por el cambio climático, que ponen a prueba su capacidad de supervivencia. El cambio en la composición genética de las poblaciones reside en las mutaciones, que son la fuente para la evolución y la adaptación a los cambios. La diversidad genética intrapoblacional está regulada por dos grandes fuerzas evolutivas que cambian la composición genética permitiendo así el acceso a nuevos fenotipos: la deriva genética y la selección natural. Por un lado, la deriva genética fija mutaciones en la población de manera aleatoria e independiente del efecto que suponga dicha mutación para la población.…
Transmisión colectiva de enterovirus asociada a membranas: Bases genéticas e implicaciones evolutivas
2021
Los virus son responsables de numerosas enfermedades. Muchas de éstas son crónicas y se estima que el 20% de los cánceres son causados por infecciones virales. Además, gracias a su gran capacidad adaptativa y a su facilidad de transmisión, tienen cierta facilidad para saltar de una especie a otra, por lo que muchos virus que infectan animales son potenciales zoonosis confiriéndoles un considerable potencial pandémico. Estudiar y comprender mejor a los virus es un paso esencial en la elaboración de mejores protocolos de salud y epidemiología, así como en la búsqueda de nuevos fármacos antivirales. En los últimos años se ha descubierto que los virus pueden transmitirse en grupo de un huésped …
A decomposition approach for multidimensional knapsacks with family-split penalties
2022
The optimization of Multidimensional Knapsacks with Family-Split Penalties has been introduced in the literature as a variant of the more classical Multidimensional Knapsack and Multi-Knapsack problems. This problem deals with a set of items partitioned in families, and when a single item is picked to maximize the utility, then all items in its family must be picked. Items from the same family can be assigned to different knapsacks, and in this situation split penalties are paid. This problem arises in real applications in various fields. This paper proposes a new exact and fast algorithm based on a specific Combinatorial Benders Cuts scheme. An extensive experimental campaign computational…
Formalin treatment increases the stability and immunogenicity of coxsackievirus B1 VLP vaccine
2019
Type B Coxsackieviruses (CVBs) are a common cause of acute and chronic myocarditis, dilated cardiomyopathy and aseptic meningitis. However, no CVB-vaccines are available for human use. We have previously produced virus-like particles (VLPs) for CVB3 with a baculovirus-insect cell production system. Here we have explored the potential of a VLP-based vaccine targeting CVB1 and describe the production of CVB1-VLPs with a scalable VLP purification method. The developed purification method consisting of tangential flow filtration and ion exchange chromatography is compatible with industrial scale production. CVB1-VLP vaccine was treated with UV-C or formalin to study whether stability and immuno…
A comparative study of the effect of UV and formalin inactivation on the stability and immunogenicity of a Coxsackievirus B1 vaccine
2019
Type B Coxsackieviruses (CVBs) belong to the enterovirus genus, and they cause both acute and chronic diseases in humans. CVB infections usually lead to flu-like symptoms but can also result in more serious diseases such as myocarditis, aseptic meningitis and life-threatening multi-organ infections in young infants. Thus, CVBs have long been considered as important targets of future vaccines. We have previously observed CVB1 capsid disintegration and virus concentration decrease with 12-day long formalin inactivation protocol. Here a scalable ion exchange chromatography purification method was developed, and purified CVB1 was inactivated with UV-C or formalin. Virus morphology and concentra…
Development of novel probes for enterovirus B group to study uncoating and infection
2016
Enterovirus B group (EV-B) viruses are important human pathogens which cause a variety of diseases from mild respiratory illnesses to more severe acute infections such as myocarditis and meningitis. EV-Bs have also been associated with chronic infections and autoimmune diseases such as type I diabetes. Because of their significance, better and more accurate methods are necessary to track and visualize viruses in vitro and in vivo. This thesis focus on the development of novel probes to label viruses site-specifically and track infection in vitro. First, we established a covalent conjugation between gold nanocluster markers and EV-B viruses. We were able to visualize through electron microsc…