Search results for "Satu"
showing 10 items of 1367 documents
Anandamide-induced apoptosis in Chang liver cells involves ceramide and JNK/AP-1 pathway
2006
In the present study we demonstrate that anandamide, the most important endogenous cannabinoid, markedly induced apoptosis in Chang liver cells, an immortalized non-tumor cell line derived from normal liver tissue, while it induced only modest effects in a number of hepatoma cell lines. The apoptotic effect was reduced by methyl-beta-cyclodextrin, a membrane cholesterol depletor, suggesting an interaction between anandamide and the membrane microdomains named lipid rafts. Anandamide effects were mediated by the production of ceramide, as demonstrated by experiments performed with the sphingomyelinase inhibitor, desipramine, or with the sphingomyelinase activator, melittin. This conclusion w…
Japānas krūmcidonijas Chaenomeles japonica šūnu suspensijas kultūras raksturošana pielietošanai biotehnoloģijā
2021
Darba mērķis bija raksturot Japānas krūmcidonijas Chaenomeles japonica suspensiju kultūru un izvērtēt tās pielietojumu biotehnoloģijā. Darbā no C. japonica kallusu kultūrām iegūtas suspensiju kultūras, veikta šūnu vizualizācija ar fluorescences mikroskopijas metodēm. Konstruēta augšanas līkne, no kuras ievākti paraugi antiradikālās aktivitātes, kopējo fenolu un antocianīnu satura noteikšanai. Lielāko fenolu savienojumu koncentrāciju un augstāko antiradikālo un antimikrobiālo aktivitāti uzrādīja stacionārās fāzes paraugi. Lielāko antocianīnu saturu uzrādīja astotās dienas paraugs. Šūnas suspensijās agregātos bija lielākoties dediferencējušās.
Organic synthesis of high added value molecules with MOF catalysts
2020
Recent examples of organic synthesis of fine chemicals and pharmaceuticals in confined spaces of MOFs are highlighted and compared with silica-based ordered porous solids, such as zeolites or mesoporous (organo)silica. These heterogeneous catalysts offer the possibility of stabilizing the desired transition states and/or intermediates during organic transformations of functional groups and (C-C/C-N) bond forming steps towards the desired functional high added value molecular scaffolds. A short introduction on zeolites, mesoporous silica and metal-organic frameworks is followed by relevant applications in which confined active sites in the pores promote single or multi-step organic synthesis…
Ring diameter effects on determination of field-saturated hydraulic conductivity of different loam soils
2017
Abstract Establishing ring diameter effects on the field-saturated soil hydraulic conductivity, Kfs, determined with ponding infiltrometer methods can help to find a compromise between the need to sample a large area with an individual measurement and the impracticality of using large rings in the field. Five ring sizes (diameter, D = 5.5, 10.9, 16.0, 27.8 and 31.8 cm) were used to determine Kfs by the simplified falling head (SFH) technique in four loamy soils with different salinity (electrical conductivity of saturated extract, ECe = 0.9–29.4 dS/m) and sodicity (exchangeable sodium percentage, ESP = 2.7–81.3%) levels. According to USDA classification, two soils were non-saline, non-sodic…
Effects of temperature on electron paramagnetic resonance of dangling oxygen bonds in amorphous silicon dioxide
2011
The properties of electron paramagnetic resonance (EPR) signal of oxygen dangling bonds in amorphous SiO2 ("non-bridging oxygen hole centers", NBOHC) in excimer laser-irradiated amorphous SiO2 were studied in the temperature range 20K to 295K. NBOHCs strongly affect optical and chemical properties of amorphous SiO2 -based (nano) structures and their surfaces. The behaviour of their EPR signal is complicated due to a nearly degenerate electronic ground state. It was found that EPR signal has a non-Curie (~1/T) T-dependence down to 40K, indicating that EPR-based concentration estimates routinely obtained at T = 77K underestimate the center concentrations at least by a factor of 1.7. The estim…
Analysis of eicosapentaenoic and docosahexaenoic acid geometrical isomers formed during fish oil deodorization.
2006
International audience; Addition of long-chain polyunsaturated fatty acids (LC-PUFAs) from marine oil into food products implies preliminary refining procedures of the oil which thermal process affects the integrity of LC-PUFAs. Deodorization, the major step involving high temperatures, is a common process used for the refining of edible fats and oils. The present study evaluates the effect of deodorization temperature on the formation of LC-PUFA geometrical isomers. Chemically isomerized eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were used as reference samples. Fish oil samples have been deodorized at 180, 220 and 250 °C for 3 h and pure EPA and DHA fatty acid methyl esters…
Chemical composition of the essential oil of Salvia microstegia Boiss. et Balansa growing wild in Lebanon.
2005
The essential oil of aerial parts of Salvia microstegia Boiss. et Balansa (Lamiaceae) growing wild in Lebanon was obtained by hydrodistillation and was analysed by GC and GC–MS. 70 compounds, representing 94.0% of the oil, were identified. The major components were caryophyllene oxide (6.2%), pulegone (5.7%), 4-vinylguaiacole (5.3%), hexadecanoic acid (5.1%) and menthone (4.9%). © 2006 Elsevier B.V. All rights reserved.
Gas chromatography-fourier transform infrared spectrometry of fatty acids: New Applications with a direct deposition interface
1998
Infrared spectroscopy is a suitable spectroscopic method to differentiate geometric Z and E isomers of unsaturated compounds. A direct-deposition Fourier transform infrared spectrometer (FTIR), coupled to a gas chromatograph, was used successfully to analyze with a high sensitivity traces of C18:1 fatty acid methyl ester (FAME) isomers. It could also conclusively distinguish between isomers of conjugated diunsaturated FAME. The achievable sensitivity of this direct-deposition device makes possible accurate FAME mixture analyses that are not currently attainable with the more conventional light-pipe interface.
Immobilization of reagents by polymeric materials. Determination of metamizol
1992
Abstract A method for immobilization of inorganic reagents, based on the dispersion of the reagent into an unsaturated polyester solution is applied to immobilization of lead dioxide. The obtained solid is of application in a flow-injection manifold for indirect atomic absorption determination of metamizol in pharmaceutical formulations. The procedure gives a linear calibration graph up to 6 ppm of metamizol with a relative standard deviation of 1.6% (3.0 mg/l) and a sample throughput of 72 hr−1.
Electrothermal Atomic Absorption Spectrometric Diagnosis of Familial Hypercholesterolemia
2000
We have developed a new nonradioactive assay to identify human low-density lipoprotein receptor defects. It is based on the incubation of cultured cells with colloidal gold-LDL conjugates and quantitation of the gold associated with the cells by electrothermal atomic absorption spectrometry. After an oxidative treatment with nitric and hydrochloric acids, the biological matrix interferes neither with the gold recovery nor with the gold measurements, which are linear, at least from 0.15 to 3 ng of gold. When cells expressing a functional LDL receptor are incubated with increasing amounts of colloidal-gold LDL conjugates, the obtained saturation curve parallels that described when [125I]LDL i…