Search results for "Scaffold"

PLLA scaffolds based on thermally induced phase separation: morphology, cell seeding and proliferation

2009

PLLA biodegradable scaffolds for Vascular Tissue Engineering (VTE) applications via dip drawing and Diffusion Induced Phase Separation (DIPS)

2009

Physical and biological properties of electrospun poly(d,l‐lactide)/nanoclay and poly(d,l‐lactide)/nanosilica nanofibrous scaffold for bone tissue en…

2021

Abstract Electrospun scaffolds exhibiting high physical performances with the ability to support cell attachment and proliferation are attracting more and more scientific interest for tissue engineering applications. The inclusion of inorganic nanoparticles such as nanosilica and nanoclay into electrospun biopolymeric matrices can meet these challenging requirements. The silica and clay incorporation into polymeric nanofibers has been reported to enhance and improve the mechanical properties as well as the osteogenic properties of the scaffolds. In this work, for the first time, the physical and biological properties of polylactic acid (PLA) electrospun mats filled with different concentrat…

Polyactide Biodegradable Scaffolds for Tissue Engineering Applications: Phase Separation-Based Techniques

2011

One of the most reliable techniques for the preparation of biodegradable scaffolds suitable for tissue engineering applications (e.g. regeneration of wounded/damaged tissues) is based on liquid/liquid phase separation of ternary solvent/antisolvent/polymer solutions. In particular, two phase separation protocols are examined here: Thermally Induced Phase Separation (TIPS) and Diffusion Induces Phase Separation (DIPS). According to the former protocol, a thermodynamically stable polymeric ternary solution is brought below its metastability/instability point (spinodal/binodal curve) by quench in a cooling medium: under opportune conditions, a foam-like structure is formed by nucleation and 3-…

PREPARATION AND CHARACTERIZATION OF POLYLACTIC ACID/SILICA AND POLYLACTIC ACID/CLAYS BIONANOCOMPOSITE ELECTROSPUN SYSTEMS

2019

Introduction Electrospinning is a versatile process technology for the production of fibers ranging from nano- to micro-scale. In order to develop high performance electrospun systems, the use of nanofillers is achieving more and more interest. In this context, silica (Si) and clays (Cl) are often used as fillers due to the possibility to combine the advantages of polymeric materials, such as light weight and flexibility, and inorganic materials, such as high mechanical strength, heat stability, and chemical stability. In this work, the morphology of PLA/Si and PLA/Cl were evaluated on two different weight ratios: 99/1 and 95/5 wt/wt. Material and Methods The nanoparticles were added to a s…

Biocompatibility and biodegradability of electrospun phea-pla scaffolds: Our preliminary experience in a murine animal model

2012

We obtained a nano-fibrillar scaffold starting from a polymeric solution which, through electrospinning, gave a biodegradable material with optimal mechanical features and the capacity to allow cell adhesion. In this paper we report the in-vivo application on a murine animal model of two electrospun biodegradable materials, specifically designed to create tubular structures. In one case PHEA-PLA was co-spun with silk fibroin (Fibro-PHEAPLA) by a parallel electrospinning process to obtain a scaffold with two different polymeric fibers. In the other case, PHEA-PLA was mixed with polycaprolactone (PCLPHEA-PLA) to obtain a hybrid fibers scaffold. The in-vitro assay showed fibroblast colonizatio…

SCAFFOLD SINTETICO DI PHEA-PLA-PCL BIOCOMPATIBILE E BIOFUNZIONALE. STUDI PRELIMINARI SU MODELLO ANIMALE. NUOVE PROSPETTIVE NELLA CHIRURGIA RIGENERATI…

2016

Introduzione: L’ingegneria tissutale ha sviluppato polimeri sintetici biocompatibili e biofunzionali, capaci di fornire una struttura tridimensionale che supporti la crescita di nuovo tessuto autologo, mediante la migrazione di cellule del ricevente. Obiettivo del nostro studio è stato valutare la resistenza di uno scaffold di PHEA-PLA-PCL ai succhi biliari di un modello animale, per determinarne l’applicabilità nella chirurgia rigenerativa delle vie biliari (VB). Metodologia: Abbiamo utilizzato 10 conigli New Zealand (n=10 )di 1,5 kg di peso. Si è proceduto all’impianto di un innesto di circa 1,5 cm2 di scaffold polimerico, costituito da polimeri di PHEA-PLA-PCL, all’interno di una colecis…

A tissue-engineered artificial bile duct grown to resemble the native bile duct in a porcine model

Hydrogel scaffolds blends to host Spheroids from human adipose stem cells

INTRODUCTION Adipose stem cells represent a reliable source of stem cells for their widely demonstrated potential in regenerative medicine and tissue engineering applications. New recent insights show that 3D models may properly mimic the native tissue properties; in fact Spheroids from Adipose derived Stem Cells (S-ASCs) displayed enhanced regenerative abilities if compared to 2D models. Stem cell therapy success is determined by "cell-quality" thus the involvement of stress signals and cellular aging need to be deeply investigated. The development of 3D cell-laden hydrogels has enabled to mimic the peculiar scenario of a native tissue. We studied SASCs-cell quality and tested their viabil…

The role of parents in the engagement of young children with digital technologies: Exploring tensions between rights of access and protection, from ‘…

2016

This study investigates the role played by parents as mediators of young children’s access and engagement with digital technologies. In Belgium, Germany, Latvia and Portugal, qualitative in-depth interviews were conducted with 10 families in each country, including one child between 6 and 7 years old. Our findings show that parents of young children mainly play the role of ‘gatekeepers’ when it comes to facilitating and constraining access to and use of digital technologies. Parents’ perceptions of the efficacy of digital technologies as responsible entertainment and as educational tools influence the technologies available at home and accessible to the child. These perceptions in turn impa…