Search results for "Scanning microscopy"

showing 10 items of 37 documents

Crystal Growth of HgSe by the Cold Travelling Heater Method

2002

We report on the low-temperature synthesis and growth of HgSe crystals making use of the cold travelling heater method. Ingots so obtained are analyzed by means of scanning microscopy, including EDAX and backscattered electron modes, and X-ray diffraction techniques.

DiffractionCrystallographyCrystallinityChemistryGeneral Materials ScienceCrystal growthGeneral ChemistryBackscattered electronCondensed Matter PhysicsScanning microscopyCharacterization (materials science)Crystal Growth & Design
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Apertureless scanning near-field optical microscopy: a comparison between homodyne and heterodyne approaches

2006

International audience; In coherent homodyne apertureless scanning near-field optical microscopy (ASNOM) the background field cannot be fully suppressed because of the interference between the different collected fields, making the images difficult to interpret. We show that implementing the heterodyne version of ASNOM allows one to overcome this issue. We present a comparison between homodyne and heterodyne ASNOM through near-field analysis of gold nanowells, integrated waveguides, and a single evanescent wave generated by total internal reflection. The heterodyne approach allows for the control of the interferometric effect with the background light. In particular, the undesirable backgro…

HeterodyneImage formationPhase (waves)Near and far fieldOptical fields02 engineering and technologyInterference (wave propagation)Total internal reflection01 natural sciencesDestructive interference010309 opticsOptics0103 physical sciences[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/MicroelectronicsPhysicsTotal internal reflectionNear field opticsbusiness.industryTapered fibersNear-field opticsScanning microscopyStatistical and Nonlinear Physics021001 nanoscience & nanotechnologyAtomic and Molecular Physics and OpticsInterferometryImage formation theory[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic0210 nano-technologybusinessScanning electron microscopy
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Microradiography and confocal laser scanning microscopy applied to enamel lesions formed in vivo with and without fluoride varnish treatment

1996

The aim of the present investigation was to combine 2 techniques suitable for lesion characterization: quantitative microradiography (TMR) and confocal laser scanning microscopy (CLSM) on in vivo induced lesions with and without a fluoride varnish (Duraphat(R)) treatment. Orthodontic bands were attached to premolars to be extracted for orthodontic reasons to induce enamel caries on the buccal surfaces. In the caries development part of the study, 1 tooth of each pair received at the start 1 topical application with the fluoride varnish Duraphat(R), the contralateral tooth serving as untreated control. All teeth were extracted after 4 weeks. In the caries progression part, the premolars were…

INVIVODentistrymicroradiographychemistry.chemical_compoundPaintPremolarChemical PrecipitationScattering RadiationFluorides TopicalChildTooth DemineralizationOrthodonticsMineralsconfocal laser scanning microscopyMicroscopy ConfocalSOUNDEnamel paintCARIESdemineralizationDURAPHATmedicine.anatomical_structureLIGHTvisual_artvisual_art.visual_art_mediumDisease Progressionmedicine.symptomcarious lesionFluorideAdolescentConfocalDental CariesLesionOrthodontic Appliancesstomatognathic systemmedicineHumansBicuspidDental EnamelGeneral DentistryINSITUINVITRObusiness.industryFluoride varnishfluoride varnishBuccal administrationDemineralizationstomatognathic diseaseschemistrySodium FluoridebusinessEuropean Journal of Oral Sciences
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Testing direct neuroprotective effects of current MS therapeutic compounds with intravital two photon laser scanning microscopy

2014

Laser Scanning MicroscopyMaterials scienceNeurologyTwo-photon excitation microscopybusiness.industryImmunologyImmunology and AllergyOptoelectronicsNeurology (clinical)Current (fluid)businessNeuroprotectionJournal of Neuroimmunology
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Immunohistochemical marker for Na+ CP type Vα (C-20) and heterozygous nonsense SCN5A mutation W822X in a sudden cardiac death induced by mild anaphyl…

2009

A sudden death likely due to mild anaphylactic reaction in a young man is described. Autoptic, histologic, immunohistochemical, and laboratory findings were strongly consistent with the diagnosis of a mild anaphylactic reaction. Genetic molecular analysis, performed on formalin-fixed, paraffin-embedded tissues, showed a mutation described as W822X in a family with electrocardiographic pattern typical of Brugada Syndrome. It results in a nonsense mutation generating a truncated form of the channel protein. The mutation is due to a point substitution of a guanine with an adenine residue (G2466A). Immunohistochemistry and laser scanning confocal microscopy on sections from heart formalin-fixed…

MaleChannellopathies; Confocal laser scanning microscopy; Immunohistochemistry; Na+ CP type Vα (C-20); Sodium channel; Sudden cardiac death; W822X; Adult; Anaphylaxis; Brugada Syndrome; Fatal Outcome; Humans; Male; Muscle Proteins; Myocardium; Myocytes Cardiac; NAV1.5 Voltage-Gated Sodium Channel; Peanut Hypersensitivity; Sodium Channels; Death Sudden Cardiac; Mutation Missense; 2734; Medical Laboratory Technology; HistologyMuscle Proteinsmedicine.disease_causeSodium ChannelsSudden cardiac deathNAV1.5 Voltage-Gated Sodium ChannelNa+ CP type V[alpha] (C-20)Fatal OutcomeMissense mutationMyocytes CardiacConfocal laser scanning microscopyCP type Vα (C-20)Cellular localizationBrugada syndromeBrugada SyndromeMutationChemistrySodium channelChannellopathiesImmunohistochemistryChannellopathies; Confocal laser scanning microscopy; Immunohistochemistry; Na; +; CP type Vα (C-20); Sodium channel; Sudden cardiac death; W822XDeathMedical Laboratory TechnologyCardiologyCardiacAdultmedicine.medical_specialtyHistologyNa+ CP type V[alpha] (C-20) confocal laser scanning microscopy immunohistochemistry sodium channel channellopathies W822X sudden cardiac deathNonsense mutation2734Mutation MissenseSocio-culturaleNa+ CP type Vα (C-20)+Sudden deathPathology and Forensic MedicineInternal medicinemedicineHumansPeanut HypersensitivityNacardiovascular diseasesW822XAnaphylaxisMyocytesSodium channelMyocardiummedicine.diseaseMolecular biologySuddenSudden cardiac deathDeath Sudden CardiacMutationMissenseNa+ CP type V[alpha] (C-20); confocal laser scanning microscopy; immunohistochemistry; sodium channel; channellopathies; W822X; sudden cardiac death
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Influence of applied strain on the microstructural corrosion of AlMg2 as-cast aluminium alloy in sodium chloride solution

2012

International audience; The corrosion behavior of ISO AlMg2 (AA5052) was studied at the microscale using the Electrochemical Microcell Technique. The influence of plastic deformation on the corrosion resistance of this alloy was also examined. After polishing, pitting at small copper-enriched precipitates and structural etching were observed. After deformation, numerous slip bands were found in grains. After 5.5% plastic strain, the global electrochemical behavior of samples was significantly affected. Pitting potential was decreased in sites containing slip bands or in sites with large strain gradients (measured using microgauges deposited by lithography).

Materials science020209 energyGeneral Chemical EngineeringAlloyPolishing02 engineering and technologyPlasticityengineering.materialAL-CUCorrosionTHIN-FILMS0202 electrical engineering electronic engineering information engineeringAluminium alloyPitting corrosionPARTICLESGeneral Materials ScienceLASER-SCANNING MICROSCOPYLOCALIZED CORROSIONMetallurgyLüders bandtechnology industry and agriculture2024-T3General Chemistry021001 nanoscience & nanotechnologyELECTROCHEMICAL-BEHAVIORvisual_artPITTING CORROSIONvisual_art.visual_art_mediumengineeringINTERMETALLIC PHASESDeformation (engineering)0210 nano-technologyPIT INITIATION
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Atomic Force Microscopy Study of Yeast Cells Influenced by High Voltage Electrical Discharge

2008

Human cells are the eukaryotic ones. Simulation of wide-spectrum electromagnetic radiation influence on eukaryotic cells was performed with yeast which is usually used now in molecular biological and medical biological investigations as the ideal model of eukaryotic system. The aim of the research was to observe possible induced alterations of the cell morphology. Atomic force microscopy (AFM) and electron scanning microscopy (ESM) have been applied to image the surface of cells exposed to electromagnetic radiation.

Materials scienceAtomic force microscopyElectron scanning microscopyHigh voltageNanotechnologyElectric dischargeCell morphologyElectromagnetic radiationYeast
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Histotomography of the odontoblast processes at the dentine-enamel junction of permanent healthy human teeth in the confocal laser scanning microscope

1998

The translucency of teeth allows the non-destructive subsurface visualisation of their microstructure by confocal laser scanning microscopy (CLSM) at a level of about 150 μm below the surface. The dentine–enamel junction (DEJ) is accessible only directly adjacent to the cervix of the tooth. Therefore teeth have to be sectioned for studying marginal areas of the dental hard tissue. The potential of the technique for (pseudo) three-dimensional visualisation allows the study of an array of individual confocal images, the interpretation of which is similar to that of macroscopic tomographs (CT-scan, MRI). Additionally, the extended focus mode yields the overlay of individual confocal images in …

Materials scienceConfocal laser scanning microscopeEnamel paintConfocalAnatomyHard tissuestomatognathic diseasesDentinal TubuleOdontoblaststomatognathic systemvisual_artConfocal laser scanning microscopyvisual_art.visual_art_mediumGeneral DentistryBiomedical engineeringClinical Oral Investigations
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Strukturelle Veränderungen in säuregeätztem Schmelz im konfokalen Laser-Scanning-Mikroskop

1996

The aim of the present investigation was to elucidate structural alternations in enamel subjected to acid etch technique and treatment under remineralizing conditions by means of a new microscopy technique known as confocal laser scanning microscopy (CLSM). Blocks of enamel were treated with 37% phosphoric acid and exposed for 3 weeks to an oral environment. Tomographic CLSM images were subsequently obtained and compared with controls. CLSM proved to be a reliable, highly reproducible and simple method of qualitative assessment of structural changes occurring on the surface of enamel and in areas below the surface as deep as 100 microns or more. Structural alterations associated with acid a…

Materials scienceEnamel paintConfocal laser scanning microscopebusiness.industryfungiLight reflectionOrthodonticsTooth Remineralizationchemistry.chemical_compoundOpticsstomatognathic systemchemistryvisual_artMicroscopyConfocal laser scanning microscopyvisual_art.visual_art_mediumWide bandOral SurgerybusinessPhosphoric acidBiomedical engineeringJournal of Orofacial Orthopedics/Fortschritte der Kieferorthopädie
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Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector

2012

[EN] We report a scheme for the detector system of confocal microscopes in which the pinhole and a large-area detector are substituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal passing through the pinhole. We demonstrate the imaging capability and the optical sectioning of the system. Subtractive-imaging confocal microscopy can be implemented in a simple manner, providing superresolution and improving optical sectioning. (C) 2012 Optical Society of America

Materials scienceOptical sectioningConfocalConfocal scanning microscopylaw.inventionOpticsConfocal microscopylawOnionsMicroscopyImage Processing Computer-AssistedMicroscopyMicroscopy Confocalbusiness.industryScanning microscopyScanning confocal electron microscopyEquipment DesignAtomic and Molecular Physics and OpticsConfocal microscopyFISICA APLICADASubtraction TechniqueLight sheet fluorescence microscopyPinhole (optics)businessAlgorithmsOptics Letters
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