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RESEARCH PRODUCT
Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector
Colin J. R. SheppardA. CalatayudManuel Martínez-corralEmilio Sánchez-ortigaAna DoblasGenaro Saavedrasubject
Materials scienceOptical sectioningConfocalConfocal scanning microscopylaw.inventionOpticsConfocal microscopylawOnionsMicroscopyImage Processing Computer-AssistedMicroscopyMicroscopy Confocalbusiness.industryScanning microscopyScanning confocal electron microscopyEquipment DesignAtomic and Molecular Physics and OpticsConfocal microscopyFISICA APLICADASubtraction TechniqueLight sheet fluorescence microscopyPinhole (optics)businessAlgorithmsdescription
[EN] We report a scheme for the detector system of confocal microscopes in which the pinhole and a large-area detector are substituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal passing through the pinhole. We demonstrate the imaging capability and the optical sectioning of the system. Subtractive-imaging confocal microscopy can be implemented in a simple manner, providing superresolution and improving optical sectioning. (C) 2012 Optical Society of America
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-01 | Optics Letters |