Search results for "Scanning"
showing 10 items of 1808 documents
Translation of hepatitis B virus (HBV) surface proteins from the HBV pregenome and precore RNAs in Semliki Forest virus-driven expression.
2004
Hepatitis B virus (HBV) pregenome RNA (pgRNA) serves as a translation template for the HBV core (HBc) protein and viral polymerase (Pol). HBV precore RNA (pcRNA) directs the synthesis of the precore (preC) protein, a precursor of the hepatitis B e antigen (HBeAg). pgRNA and pcRNA were expressed in the Semliki Forest virus (SFV) expression system. Besides the HBc and preC proteins, there was revealed the synthesis of all three forms of HBV surface (HBs) proteins: long (LHBs), middle (MHBs) and short (SHBs), the start codons of which are located more than 1000 nt downstream of the HBc and preC start codons. Moreover, other HBV templates, such as 3′-truncated pgRNA lacking 3′ direct repeat and…
Direct chemical grafted curcumin on halloysite nanotubes as dual-responsive prodrug for pharmacological applications
2016
Covalently functionalized halloysite nanotubes (HNTs) were successfully employed as dual-responsive nanocarriers for curcumin (Cur). Particularly, we synthesized HNT-Cur prodrug with a controlled curcumin release on dependence of both intracellular glutathione (GSH) and pH conditions. In order to obtain HNT-Cur produgs, halloysite was firstly functionalized with cysteamine through disulphide linkage. Afterwards, curcumin molecules were chemically conjugated to the amino end groups of halloysite via Schiff's base formation. The successful functionalization of halloysite was proved by thermogravimetric analysis, FT-IR spectroscopy, dynamic light scattering and scanning electron microscopy. Ex…
Orientation of charged clay nanotubes in evaporating droplet meniscus.
2015
© 2014 Elsevier Inc. During drying, an aqueous suspension of strongly charged halloysite clay nanotubes concentrates at the edge of the droplet ("coffee-ring" effect) which provides alignment of the tubes along the liquid-substrate contact line. First, the surface charge of the nanotubes was enhanced by polyanion adsorption inside of the lumen to compensate for the internal positive charges. This increased the magnitude of the ξ-potential of the tubes from -36 to -81mV and stabilized the colloids. Then, colloidal halloysite was dropped onto the substrate, dried at 65°C and after a concentration of ~0.05mgmL-1 was reached, the alignment of nanotubes occurred starting from the droplet edges. …
Synthesis of fluorinated oxadiazoles with gelation and oxygen storage ability
2012
A new family of fluorinated low molecular weight (LMW) gelators has been synthesized through SNAr substitution of 5-polyfluoroaryl-3-perfluoroheptyl-1,2,4-oxadiazoles with glycine ester. The obtained compounds give thermal and pH-sensitive hydrogels or thermo-reversible organogels in DMSO. Oxygen solubility studies showed the ability to maintain high oxygen levels in solution and in gel blend with plate counter agar (PCA).
Low Cost Handheld 3D Scanning for Architectural Elements Acquisition
2016
3D scanning has gone a long way since its first appearance in cultural heritage digitization and modeling. In the recent years some new low cost, fast, accurate emerging technologies are flooding the market. Envisioning the massive use of these cheap and easy to use devices in the next years, it is crucial to explore the possible fields of application and to test their effectiveness in terms of easiness of 3D data collection, processing, mesh resolution and metric accuracy against the size and features of the objects. In this study we focus the attention on one emerging technology, the Structure Sensor device, in order to verify a 3D pipeline acquisition on an architectural element and its …
Structural Domains of the Bacillus thuringiensis Vip3Af Protein Unraveled by Tryptic Digestion of Alanine Mutants
2019
Vip3 proteins are increasingly used in insect control in transgenic crops. To shed light on the structure of these proteins, we used the approach of the trypsin fragmentation of mutants altering the conformation of the Vip3Af protein. From an alanine scanning of Vip3Af, we selected mutants with an altered proteolytic pattern. Based on protease digestion patterns, their effect on oligomer formation, and theoretical cleavage sites, we generated a map of the Vip3Af protein with five domains which match some of the domains proposed independently by two in silico models. Domain I ranges amino acids (aa) 12&ndash
Effects of ozone on the foliar histology of the mastic plant (Pistacia lentiscus L.)
2004
[EN] An open-top chamber study was conducted to investigate the tissue and cellular-level foliar effects of ozone (O-3) on a Mediterranean evergreen species, the mastic plant (Pistacia lentiscus L.). Plants were exposed at three different O-3 levels, and leaf samples were collected periodically from the beginning of the exposure. Although no visible foliar injury was evident, alterations of the plastids and vacuoles in the mesophyll were observed. Senescence processes were accelerated with an anomalous stacking of tannin vacuoles, and a reduction in the size and number of the chloroplasts. Overall, most of the modifications induced by O-3 were consistent with previously reported observation…
20-Hydroxyecdysone-induced differentiation and apoptosis in the Drosophila cell line, l(2)mbn
2001
20-Hydroxyecdysone has an inhibitory effect on the proliferation of l(2)mbn cells, causes vacuolization and fragmentation of cells, and promotes a strong phagocytotic activity. From several lines of evidence, it can be concluded that 20-hydroxyecdysone induces apoptosis. Long-term video observations following the fate of individual cells, scanning and transmission electron microscopy reveal the typical characteristics of apoptosis: sequestration of small cellular protuberances or larger parts of the cell with nuclear fragments (apoptotic bodies), chromatin condensation, condensation and vesiculation of cytoplasm, whereas the mitochondria retain their normal appearance. The induction of apop…
A lytic mechanism based on soluble phospholypases A2 (sPLA2) and b-galactoside specific lectins is exerted by Ciona intestinalis (ascidian) unilocula…
2011
Abstract Hemocytes from the ascidian Ciona intestinalis exert in vitro Ca 2+ -dependent cytotoxic activity toward mammalian erythrocytes and K562 cells. To examine the lytic mechanism, hemocyte populations were separated (B1–B6 bands) through a Percoll discontinuous density gradient, the hemocyte cytotoxic activity (HCA) and the lytic activity of the hemocyte lysate supernatant (HLS) were assayed. In addition the separated hemocytes were cultured and the cell-free culture medium (CFM) assayed after 3 h culture. Results support that unilocular refractile hemocytes (URGs), enriched in B5, are cytotoxic. The B5-HLS contains lysins and the activity of B5-CFM shows that lysins can be released in…
Apertureless scanning near-field optical microscopy: a comparison between homodyne and heterodyne approaches
2006
International audience; In coherent homodyne apertureless scanning near-field optical microscopy (ASNOM) the background field cannot be fully suppressed because of the interference between the different collected fields, making the images difficult to interpret. We show that implementing the heterodyne version of ASNOM allows one to overcome this issue. We present a comparison between homodyne and heterodyne ASNOM through near-field analysis of gold nanowells, integrated waveguides, and a single evanescent wave generated by total internal reflection. The heterodyne approach allows for the control of the interferometric effect with the background light. In particular, the undesirable backgro…