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RESEARCH PRODUCT

Direct chemical grafted curcumin on halloysite nanotubes as dual-responsive prodrug for pharmacological applications

Renato NotoRiccardo AmoratiGiuseppe LazzaraStefana MiliotoMarina MassaroSerena RielaPaola PomaGiuseppe CavallaroSusanna Guernelli

subject

Halloysite nanotubeAntiproliferative activity02 engineering and technology01 natural scienceshalloysite nanotubes covalent functionalization curcumin prodrugchemistry.chemical_compoundColloid and Surface ChemistryOrganic chemistryProdrugsProdrugSettore CHIM/02 - Chimica FisicaDrug CarriersNanotubesChemistryAntioxidant propertieFree Radical ScavengersSurfaces and InterfacesGeneral MedicineProdrug021001 nanoscience & nanotechnologyDrug deliveryAluminum Silicates0210 nano-technologyDrug carrierOxidation-ReductionBiotechnologyCurcuminCell SurvivalAntineoplastic AgentsHalloysite nanotubes Curcumin Prodrug Antiproliferative activity Antioxidant propertiesengineering.materialConjugated system010402 general chemistryHalloysiteCell Line TumorHumansPhysical and Theoretical ChemistryCell ProliferationSettore CHIM/06 - Chimica OrganicaCombinatorial chemistry0104 chemical sciencesKineticsMicroscopy Electron ScanningengineeringCurcuminSettore BIO/14 - FarmacologiaClayPharmaceuticsNanocarriers

description

Covalently functionalized halloysite nanotubes (HNTs) were successfully employed as dual-responsive nanocarriers for curcumin (Cur). Particularly, we synthesized HNT-Cur prodrug with a controlled curcumin release on dependence of both intracellular glutathione (GSH) and pH conditions. In order to obtain HNT-Cur produgs, halloysite was firstly functionalized with cysteamine through disulphide linkage. Afterwards, curcumin molecules were chemically conjugated to the amino end groups of halloysite via Schiff's base formation. The successful functionalization of halloysite was proved by thermogravimetric analysis, FT-IR spectroscopy, dynamic light scattering and scanning electron microscopy. Experimental data confirmed the presence of curcumin on HNT external surface. Moreover, we investigated the kinetics of curcumin release by UV-vis spectroscopy, which highlighted that HNT-Cur prodrug possesses dual stimuli-responsive ability upon exposure to GSH-rich or acidic environment. In vitro antiproliferative and antioxidant properties of HNT-Cur prodrug were studied with the aim to explore their potential applications in pharmaceutics. This work puts forward an efficient strategy to prepare halloysite based nanocarriers with controlled drug delivery capacity through direct chemical grafting with stimuli-responsive linkage.

yearjournalcountryeditionlanguage
2016-01-01
10.1016/j.colsurfb.2016.01.025http://hdl.handle.net/10447/180968