Search results for "Screening"

Synthesis and Antitumor Activity of New Thiazole Nortopsentin Analogs

2016

New thiazole nortopsentin analogs in which one of the two indole units was replaced by a naphthyl and/or 7-azaindolyl portion, were conveniently synthesized. Among these, three derivatives showed good antiproliferative activity, in particular against MCF7 cell line, with GI50 values in the micromolar range. Their cytotoxic effect on MCF7 cells was further investigated in order to elucidate their mode of action. Results showed that the three compounds act as pro-apoptotic agents inducing a clear shift of viable cells towards early apoptosis, while not exerting necrotic effects. They also caused cell cycle perturbation with significant decrease in the percentage of cells in the G0/G1 and S ph…

Synthesis of the New Ring System Bispyrido[4',3':4,5]pyrrolo [1,2-a:1',2'-d]pyrazine and Its Deaza Analogue

2014

Derivatives of the new ring systems bispyrido[4',3':4,5]pyrrolo[1,2-a:1',2'-d] pyrazine-6,13-dione and its deaza analogue pyrido[4'',3'':4',5']pyrrolo-[1',2':4,5]pyrazino [1,2-a]indole-6,13-dione were conveniently synthesized through a four-step sequence. Symmetrical derivatives of the former ring system were obtained through self condensation. On the other hand, condensation of 6-azaindole carboxylic acid with indole 2-carboxylic acid afforded the deaza analogue ring system. Derivatives of the title ring system were tested by the National Cancer Institute (Bethesda, MD, USA) and four of them exhibited modest activity against MCF7 (a breast cancer cell line) and/or UO-31 (a renal cancer cel…

3-(6-Phenylimidazo [2,1-

2019

A new series of imidazo[2,1-b][1,3,4]thiadiazole derivatives was efficiently synthesized and screened for their in vitro antiproliferative activity on a panel of pancreatic ductal adenocarcinoma (PDAC) cells, including SUIT-2, Capan-1 and Panc-1. Compounds 9c and 9l, showed relevant in vitro antiproliferative activity on all three pre-clinical models with half maximal inhibitory concentration (IC50) ranging from 5.11 to 10.8 µM, while the compounds 9e and 9n were active in at least one cell line. In addition, compound 9c significantly inhibited the migration rate of SUIT-2 and Capan-1 cells in the scratch wound-healing assay. In conclusion, our results will support further studies to increa…

Adhesión a las guías clínicas para el screening del cáncer de próstata en Atención Primaria e identificación de áreas de mejora

2021

El cáncer de próstata es la segunda neoplasia más frecuente y la quinta causa de muerte por cáncer en varones. Desde la introducción del antígeno prostático específico (PSA) en 1979 hemos vivido un aumento en la incidencia de esta neoplasia; sin embargo, el hecho de que sea un tumor con un periodo de latencia muy prolongado ha llevado a una confusión en cuanto a conveniencia del diagnóstico y tratamiento de pacientes en estadios tempranos. Clásicamente, ninguna de las sociedades de Urología europeas ni americanas han recomendado el screening de cáncer de próstata dejando a criterio del clínico el diagnóstico precoz oportunista del mismo, hasta el punto que la United States Preventive Servic…

Antagonism and Antimicrobial Capacity of Epiphytic and Endophytic Bacteria against the Phytopathogen Xylella fastidiosa

2022

Olive quick decline syndrome (OQDS), which is caused by Xylella fastidiosa, poses a severe threat to the agriculture of Mediterranean countries and causes severe damage to the olive trees in Italy. Since no effective control measures are currently available, the objective of this study was the screening of antagonistic bacteria that are potentially deployable as biocontrol agents against X. fastidiosa. Therefore, two approaches were used, i.e., the evaluation of the antagonistic activity of (i) endophytic bacteria isolated from two different cultivars of olive trees (Leccino and Ogliarola salentina) and (ii) epiphytic bacteria isolated from the phyllospheres of different host plant species …

Spirocurcasone, a diterpenoid with a novel carbon skeleton from Jatropha curcas.

2010

Spirocurcasone (14), a diterpenoid possessing the unprecedented "spirorhamnofolane" skeleton, was isolated from the root barks of Jatropha curcas, a plant extensively cultivated throughout the world, along with 11 known and two other new diterpenoids. The stereostructure of spirocurcasone was established using HRESIMS, NMR, and quantum mechanical calculation of the electronic circular dichroic (ECD) spectrum. Some of the isolated diterpenoids showed a potent activity against L5178Y, a mouse lymphoma cell line.

AB1358 Infectious antibodies repertoire in rheumatoid arthritis

2018

Background The incidence of infectious diseases in the RA ADAPTHERA study by ELISA antibody screening and the differences in infectious distribution in active or low active disease patients was explored. Methods Sera from 88 naive RA patients out of the ADAPTHERA study cohort, disease duration Results 82% RA patients were found to be positive for HSV1 +2 IgG (2% IgM positive), 8% for Adenovirus (IgA), 77% (IgG), and 1% (IgM). 99% for EBV-IgG (no IgM positive). 53% (IgG), and 26% (IgM) for CMV. 38% for HP-IgG and 15% for IgA and 79% for P-B19-IgG (3% IgM). 6% for Borrelia-IgM and 14% for IgG. A slightly increase was found for EBV sera positivity (99% IgG), compared to the normal population. …

Cloning of cDNAs coding forCandida albicanscell surface proteins

1995

Two cDNA libraries were constructed from mRNAs obtained from yeast cells and germ-tubes of Candida albicans in lambda gt11. Immunoscreening with polyclonal antibodies raised against cell wall components allowed the detection of 29 positive clones. Two of these clones were selected for their specific reactivity with antisera either from yeast (clone 11Y) or germ-tubes (clone 24M). cDNA fragments were isolated by the digestion of lambda DNA with EcoRI. Southern blot analysis with these fragments as probes demonstrated homology with C. albicans DNA, and by Northern analysis two mRNAs transcripts were detected with sizes of approximately 1·5 kb for 11Y and 1·1 kb for 24M. Both transcripts were …

Inkjet printing arrays and their applications for drug screening

2010

Krūts vēža attīstība Latvijā pēc valsts krūts vēža skrīninga programmas ieviešanas

2020

Latvijā sieviešu saslimstība un mirstība no krūts vēža ieņem pirmo vietu starp visiem ļaundabīgiem audzējiem. Katru gadu saslimšana tiek pirmreizēji reģistrēta aptuveni 1100 pacientēm un ikgadu no krūts vēža mirst vairāk nekā 400 sievietes. Kopš 2009. gadā Latvijā darbojas valsts krūts vēža skrīnings ar mērķi uzlabot krūts vēža agrīnu diagnostiku, tomēr vidēji 32 % gadījumos krūts vēzis tiek diagnosticēts novēloti slimības III un IV stadijā (SPKC, 2017). Darba tēma ir aktuāla un darba rezultāti ļauj sniegt rekomendācijas agrīnas diagnostikas uzlabošanai un skrīninga programmas uzlabošanai. Darba mērķis: veikt Latvijas valsts apmaksāta krūšu skrīninga efektivitātes izpēti laika posmā no 2010…