Search results for "Secondary"

Activation of bee venom phospholipase A2 through a peptide-enzyme complex

1995

AbstractPhospholipase A2 activation by membrane-bound peptides was investigated in order to understand the role of the membrane-induced conformation on activation, and to examine the occurrence of a peptide-enzyme complex at the lipid/water interface. For the peptides studies, bee venom phospholipase A2 was stimulated regardless of the membrane-bound conformation (α-helix, β-sheet or random coil). Using antisera raised against melittin, we were able to demonstrate the occurrence of a calcium-dependent complex involving the enzyme, phospholipid substrate, and peptide.

Controlled trial for long-term low-dose erythromycin after sinus surgery for chronic rhinosinusitis

2014

Objectives/Hypothesis The efficacy of macrolides in chronic rhinosinusitis (CRS) is still under controversy. To date, only two double-blind, placebo-controlled studies have been published with differing results. None of these studies investigated the possible benefit of macrolides in the postoperative period. We conducted an investigator-initiated clinical trial using 250-mg erythromycin once a day over a period of 3 months, beginning the administration of either erythromycin or placebo 2 weeks after a surgical intervention for CRS. Study Design Randomized double-blind, placebo-controlled trial. Methods The concentrations of eosinophilic cationic protein (ECP) and myeloperoxidase in nasal s…

Synthesis and Structure-Activity Relationships of Amino Acid Conjugates of Cholanic Acid as Antagonists of the EphA2 Receptor

2013

The Eph–ephrin system plays a critical role in tumor growth and vascular functions during carcinogenesis. We had previously identified cholanic acid as a competitive and reversible EphA2 antagonist able to disrupt EphA2-ephrinA1 interaction and to inhibit EphA2 activation in prostate cancer cells. Herein, we report the synthesis and biological evaluation of a set of cholanic acid derivatives obtained by conjugation of its carboxyl group with a panel of naturally occurring amino acids with the aim to improve EphA2 receptor inhibition. Structure-activity relationships indicate that conjugation of cholanic acid with linear amino acids of small size leads to effective EphA2 antagonists whereas …

Construction of an agglutination tool: recombinant Fab fragments biotinylated in vitro.

2009

The pComb3H vector system is used for constructing and panning recombinant antibody libraries. It allows for expression of monovalent Fab fragments, either on the surface of M13 phage, or in the form of soluble proteins secreted into the periplasmic space of bacteria. We constructed a modified pComb3H vector containing cDNA encoding for a 23-amino acid fragment of the Escherichia coli biotin carboxy carrier protein (BCCP), which is an acceptor sequence for biotinylation. The vector was used to express the Fab fragment recognizing human glycophorin A. The purified Fab fragment containing this biotin acceptor sequence was effectively biotinylated in vitro using biotin ligase (BirA). The speci…

Análisis de los modelos matemáticos producidos durante la resolución de problemas de Fermi

2017

[EN] In this paper, we present an analysis of written productions of 16 year-old students while solving Estimation Problems involving Big Numbers (EPiBN). This kind of problems is a particular type of Fermi problems and allows us to introduce modelling processes in Secondary school classrooms. Our analysis supports on the characterization of mathematical models developed by students based on the model definition of Lesh and Harel. The results show that, through the analysis of EPiBN resolutions, differentiating aspects can be distinguished between the models produced by students without modelling experience of those produced by students with prior experience, especially in the concepts and …

SP619The effect of etelcalcetide in hemodialysis patients with secondary hyperparathyroidism in daily clinical practice. A prospective multicenter st…

2019

Successful principalship in Norway: sustainable ethos and incremental changes?

2009

PurposeThe purpose of this paper is to explore whether and how success has been sustained over time in schools which were identified as being successful five years ago.Design/methodology/approachThree schools were selected for a revisit, and the sample included two combined schools (grade 1‐10) and one upper secondary school (grade 11‐13). In two schools the same principals were still in post, and in the third school there had recently been a change in principalship. Interviews with the principal and a group of teachers at each school were the major source of new data. Questions that guided the study: What structural and cultural changes can be identified within the schools compared with fi…

Secondary structure of peptides. 4:13C-Nmr CP/MAS investigation of solid oligo- and poly(L-alanines)

1983

Primary and tertiary amine-initiated polymerizations of L-alanine-N-carboxyanhydride (L-Ala-NCA) were conducted at 20 or 100°C in a variety of solvents. The 75.5-MHz 13C-nmr CP/MAS spectra of the resulting poly(L-alanines) revealed that all samples contain both α-helix and pleated-sheet structures. Depending on the reaction conditions the α-helix content varied between ca. 1 and 99%. Reprecipitation from aprotic nonsolvents does not change the α-helix/β-sheet ratio, indicating that this ratio is thermodynamically controlled. Since relatively large amounts of oligopeptides of degree of polymerization (DP) 4–6 can be extracted by means of acetic acid, it is concluded that (a) most poly(L-alan…

Exergy recovery in regasification facilities – Cold utilization: A modular unit

2009

Abstract The paper deals with the problem of cold recovery for direct utilization both in the site of regasification facility and far from it. A modular LNG regasification unit is proposed having the regasification capacity of 2 billion standard cubic meters/year of gas. The modular plant is based on use of a power cycle working with ethane or ethylene which allows operation of cold energy transfer, contained in LNG to be regasified, in a range of temperatures suitable for multipurpose use of cold, reducing regasification process irreversibility. Some electric energy is produced by the power cycle, but the own mission of modular unit proposed is addressed to deliver cold suitable for indust…

Expansion of private secondary education : lessons from recent experience in Tanzania

2000

International audience; The private sector's role in education has been the subject of much analysis and policy debate in recent years. In developing countries, public resources for education are limited and governments have traditionnally relied on private education, particularly at the post-basic levels, to meet the excess demand for education. Even when excess demand is not a major issue, advocates of private education note that private schools can be more efficient than their public sector counterparts, delivering more value in terms of student achievement per investment of resources. In this article we explore Tanzania's experience with private education during the early 1990s, a perio…