Search results for "Secondary"

showing 10 items of 1765 documents

Molecular similarities and differences from human pulmonary fibrosis and corresponding mouse model: MALDI imaging mass spectrometry in comparative me…

2017

Animal models can reproduce some model-specific aspects of human diseases, but some animal models translate poorly or fail to translate to the corresponding human disease. Here, we develop a strategy to systematically compare human and mouse tissues, and conduct a proof-of-concept experiment to identify molecular similarities and differences using patients with idiopathic pulmonary fibrosis and a bleomycin-induced fibrosis mouse model. Our novel approach employs high-throughput tissue microarrays (TMAs) of humans and mice, high-resolution matrix-assisted laser desorption/ionization-Fourier transform-ion cyclotron resonance-mass spectrometry imaging (MALDI-FT-ICR-MSI) to spatially resolve ma…

0301 basic medicineMALDI imagingPulmonary FibrosisSecondary MetabolismComputational biologyBiologyBioinformaticsProof of Concept StudyPathology and Forensic MedicineBleomycinMice03 medical and health sciencesIdiopathic pulmonary fibrosisMetabolomicsSpecies SpecificityFibrosisAdministration InhalationSpectroscopy Fourier Transform InfraredPulmonary fibrosismedicineAnimalsCluster AnalysisHumansMetabolomicsLungPhysiology ComparativeMolecular BiologyAntibiotics AntineoplasticTissue microarrayCell BiologyCyclotronsmedicine.diseaseImmunohistochemistryDisease Models AnimalMatrix-assisted laser desorption/ionization030104 developmental biologyTissue Array AnalysisSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationImmunohistochemistryLaboratory Investigation
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Standardising clinical outcomes measures for adult clinical trials in Fabry disease: A global Delphi consensus.

2021

International audience; Background: Recent years have witnessed a considerable increase in clinical trials of new investigational agents for Fabry disease (FD). Several trials investigating different agents are currently in progress; however, lack of standardisation results in challenges to interpretation and comparison. To facilitate the standardisation of investigational programs, we have developed a common framework for future clinical trials in FD.Methods and findings: A broad consensus regarding clinical outcomes and ways to measure them was obtained via the Delphi methodology. 35 FD clinical experts from 4 continents, representing 3389 FD patients, participated in 3 rounds of Delphi p…

0301 basic medicineMaleDelphi TechniqueEndocrinology Diabetes and Metabolism[SDV]Life Sciences [q-bio]Delphi methodDisease030105 genetics & heredityKidneyBiochemistry0302 clinical medicineEndocrinologyClinical outcomesClinical Trials as TopicGlobosidesTrihexosylceramidesMiddle Aged3. Good healthClinical trialIsoenzymesTreatment OutcomeInclusion and exclusion criteriaSecondary Outcome MeasureFemaleAdultmedicine.medical_specialtyConsensusLysosomal storage disorders03 medical and health sciencesQuality of life (healthcare)Inherited metabolic disordersGeneticsmedicineHumansEnzyme Replacement TherapyIntensive care medicineMolecular BiologyFabry diseaseSphingolipidsbusiness.industryClinical study designmedicine.diseaseFabry diseaseClinical trialDelphi consensusalpha-GalactosidaseQuality of LifeFabry DiseaseGlycolipidsbusiness030217 neurology & neurosurgeryMolecular genetics and metabolism
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Longitudinal Analysis of Serum Autoantibody-Reactivities in Patients with Primary Open Angle Glaucoma and Optic Disc Hemorrhage.

2015

Background The aim of our current investigation was to analyze the autoantibody-reactivities of primary open angle glaucoma patients with optic disc hemorrhage as possibly correlated to disease progression by means of a protein microarray approach. Methods Sera of patients with primary open angle glaucoma and optic disc hemorrhage (n = 16) were collected directly after study inclusion (0 weeks) and after 2 weeks, 4 weeks and 12 weeks. As a control group patients with primary open angle glaucoma (n = 18) were used (0 weeks and 12 weeks). Microarrays were incubated and occurring antibody-antigen-reactions were visualized with fluorescence labeled anti-human-IgG secondary antibodies. To detect…

0301 basic medicineMaleRetinal Ganglion CellsSerum ProteinsVisual acuitygenetic structuresEye DiseasesMicroarraysVisionVisual AcuityGlaucomalcsh:MedicineSocial SciencesPathogenesisPathology and Laboratory MedicineVascular MedicineBiochemistryPathogenesis0302 clinical medicineAnimal CellsMedicine and Health SciencesPsychologyLongitudinal Studieslcsh:ScienceNeuronsMultidisciplinarybiologyMiddle AgedPrimary and secondary antibodiesmedicine.anatomical_structureBioassays and Physiological AnalysisDisease ProgressionFemaleSensory PerceptionAntibodymedicine.symptomCellular TypesAnatomyGlaucoma Open-AngleOptic discResearch Articlemedicine.medical_specialtyGanglion CellsOpen angle glaucomaOcular AnatomyProtein Array AnalysisHemorrhageResearch and Analysis MethodsOptic Disc03 medical and health sciencesSigns and SymptomsDiagnostic MedicineOcular SystemOphthalmologymedicineHumansAgedAutoantibodiesbusiness.industrylcsh:RAutoantibodyBiology and Life SciencesAfferent NeuronsProteinsGlaucomaCell Biologymedicine.diseaseeye diseasesOphthalmology030104 developmental biologyCellular Neuroscience030221 ophthalmology & optometrybiology.proteinlcsh:Qsense organsbusinessNeurosciencePloS one
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Effects of exercise frequency and training volume on bone changes following a multi-component exercise intervention in middle aged and older men: Sec…

2021

Progressive resistance training (PRT) combined with weight-bearing impact exercise are recommended to optimize bone health, but the optimal frequency and dose of training remains uncertain. This study, which is a secondary analysis of an 18-month intervention in men aged 50–79 years, examined the association between exercise frequency and the volume of training with changes in DXA and QCT-derived femoral neck (FN) and lumbar spine (LS) bone outcomes, respectively. Men were allocated to either thrice-weekly PRT plus impact exercise training (n = 87) or a non-exercising (n = 85) group. Average weekly exercise frequency (ExFreq) and training volume per session [PRT volume (weight lifted, kg), …

0301 basic medicineMalemedicine.medical_specialtyHistologyweight-bearing exercisePhysiologyEndocrinology Diabetes and Metabolismluuntiheys030209 endocrinology & metabolismlaw.invention03 medical and health sciences0302 clinical medicineRandomized controlled triallawBone DensitySecondary analysisMedicineHumansolder menExerciseFemoral neckAgeddose-responsekehonpainoharjoitteluLumbar VertebraeExercise interventionbusiness.industryFemur NeckResistance trainingResistance TrainingMiddle Agedkeski-ikäiset030104 developmental biologymedicine.anatomical_structurePhysical therapyLumbar spinemiehetvoimaharjoitteluresistance trainingbusinessbone mineral densityExercise frequencyikääntyneetVolume (compression)Bone
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Can a Prebiotic Formulation Reduce Frailty Levels in Older People?

2019

Objective: The purpose of this study was to examine whether a prebiotic formulation reduces frailty index (FI) levels in older people. Design: We conducted secondary analysis of a placebo-controlled, randomized, double-blind design study. Setting/Participants: The study included non-demented people over the age of 65 who were living in nursing homes and were able to walk. Fifty participants completed the study (75.3±7.3 years, 70% females). Intervention: Participants were randomly assigned to either a group who received daily Darmocare Pre® (inulin and fructooligosaccharides) for 13 weeks or a placebo group (maltodextrin). Measurement: The primary outcome in this secondary analysis was chan…

0301 basic medicineMalemedicine.medical_specialtymedicine.medical_treatmentFrail ElderlyFrailty IndexOligosaccharidesPlacebo grouplaw.invention03 medical and health sciencesPrimary outcomeRandomized controlled trialDouble-Blind MethodlawSecondary analysismedicineHumansAgedAged 80 and overFrailtybusiness.industryPrebioticInulinGeneral Medicine030104 developmental biologyPrebioticsTreatment OutcomePhysical therapyFemalebusinessOlder peopleNursing homesThe Journal of frailtyaging
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Immunogenicity and Safety of Primary and Booster Vaccinations of a Fully Liquid DTaP-IPV-HB-PRP-T Hexavalent Vaccine in Healthy Infants and Toddlers …

2018

To support a fully liquid, diphtheria (D)-tetanus (T)-acellular pertussis (aP)-inactivated poliovirus (IPV)-hepatitis B (HB)-Haemophilus influenzae b (PRP-T) vaccine in Europe using a 2, 3, 4 month primary series and a booster at 11-15 months of age. Phase III, randomized, observer-blind studies in Germany and the Czech Republic. Participants who had not received HB vaccine were randomized to a 2, 3, 4 month primary series of DTaP-IPV-HB-PRP-T (group 1; N = 266) or a reconstituted DTaP-HB-IPV//PRP-T comparator (group 2; N = 263) and a booster of the same vaccine. Pneumococcal vaccine (PCV13) and rotavirus vaccine were coadministered at 2, 3, 4 months, and the booster was coadministered with…

0301 basic medicineMicrobiology (medical)MalePediatricsmedicine.medical_specialty030106 microbiologyImmunization SecondaryBooster doseAntibodies ViralDiphtheria-Tetanus-acellular Pertussis Vaccines03 medical and health sciences0302 clinical medicineImmunogenicity VaccineSuspensionsGermanyTetanus ToxoidMedicineHumansHepatitis B Vaccines030212 general & internal medicineVaccines CombinedDiphtheria-Tetanus-acellular Pertussis VaccinesImmunization ScheduleCzech RepublicHaemophilus VaccinesBooster (rocketry)business.industryDiphtheriaImmunogenicityVaccinationInfant NewbornInfantmedicine.diseaseRotavirus vaccineAntibodies BacterialVaccinationPoliovirus Vaccine InactivatedInfectious DiseasesPneumococcal vaccinePediatrics Perinatology and Child HealthFemalebusinessThe Pediatric infectious disease journal
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'Secondary prevention' against female HPV infection: literature review of the role of carrageenan.

2020

Introduction: Human papillomaviruses (HPVs) are common sexually transmitted pathogens, causally associated with cervical cancer and other anogenital cancers, as well as approximately 20% of head and neck cancers. The HPV vaccine is an exceptional primary prevention tool, but the question of adequate secondary-prevention strategies remains open. The aim of this review is to better clarify the role of carrageenan in HPV prevention-strategies. Areas covered: A comprehensive literature search was performed (PubMed/MEDLINE, Embase, Google Scholar, Cochrane Databases) to identify articles on the use of carrageenan against HPV infection. The studies were identified using combinations of the search…

0301 basic medicineMicrobiology (medical)Settore MED/07 - Microbiologia E Microbiologia Clinicamedicine.medical_treatment030106 microbiologyCarrageenan papillomavirus prevention HPV microbicideMEDLINEAlphapapillomavirusBioinformaticsCarrageenanMicrobiology03 medical and health scienceschemistry.chemical_compound0302 clinical medicineImmune systemVirologyMicrobicidemedicineSecondary PreventionAnimalsHumans030212 general & internal medicinePapillomavirus VaccinesCervical cancerbusiness.industryMechanism (biology)Papillomavirus InfectionsHPV infectionmedicine.diseaseSettore MED/40 - Ginecologia E OstetriciaCarrageenanInfectious DiseaseschemistryFemalebusinessAdjuvantExpert review of anti-infective therapy
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Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas…

2018

15 páginas, 6 tablas, 2 figuras. -- First publication is due to Frontiers Media

0301 basic medicineMicrobiology (medical)antibiotic resistanceAntibiotic resistance030106 microbiologylcsh:QR1-502anticarcinogenicBiologyMicrobiologyNRPS-PKSantimicrobialslcsh:MicrobiologyMicrobiology03 medical and health sciencesBacteriocinPseudomonas luteaBark beetlesGenome mininggenome miningAntiviralOriginal ResearchAntimicrobialsSecondary metabolitesPseudomonas16S ribosomal RNArpoBAntimicrobialbiology.organism_classificationantiviralAnticarcinogenic030104 developmental biologybark beetlesBacteriaPseudomonas abietaniphila
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Antiviral Agents From Fungi : Diversity, Mechanisms and Potential Applications

2018

Viral infections are amongst the most common diseases affecting people worldwide. New viruses emerge all the time and presently we have limited number of vaccines and only few antivirals to combat viral diseases. Fungi represent a vast source of bioactive molecules, which could potentially be used as antivirals in the future. Here, we have summarized the current knowledge of fungi as producers of antiviral compounds and discuss their potential applications. In particular, we have investigated how the antiviral action has been assessed and what is known about the molecular mechanisms and actual targets. Furthermore, we highlight the importance of accurate fungal species identification on ant…

0301 basic medicineMicrobiology (medical)endofyytitnatural productsBioactive moleculesmedia_common.quotation_subjectantiviral mechanismslcsh:QR1-502fungal secondary metabolitesendophytesluonnontuotteetReviewComputational biologyBiology01 natural sciencesMicrobiologylcsh:Microbiology03 medical and health sciencesantiviral agentsSpecies identificationpuolustusmekanismit (biologia)media_commonantimikrobiset yhdisteet010405 organic chemistrymedicinal mushroomsta1183ta1182luonnonaineet0104 chemical sciences030104 developmental biologyvirustauditsienetDiversity (politics)
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The Generalist Inside the Specialist: Gut Bacterial Communities of Two Insect Species Feeding on Toxic Plants Are Dominated by Enterococcus sp.

2016

Some specialist insects feed on plants rich in secondary compounds, which pose a major selective pressure on both the phytophagous and the gut microbiota. However, microbial communities of toxic plant feeders are still poorly characterized. Here, we show the bacterial communities of the gut of two specialized Lepidoptera, Hyles euphorbiae and Brithys crini, which exclusively feed on latex-rich Euphorbia sp. and alkaloid-rich Pancratium maritimum, respectively. A metagenomic analysis based on high-throughput sequencing of the 16S rRNA gene revealed that the gut microbiota of both insects is dominated by the phylum Firmicutes, and especially by the common gut inhabitant Enterococcus sp. Staph…

0301 basic medicineMicrobiology (medical)media_common.quotation_subject030106 microbiologyInsectGut floraGeneralist and specialist speciesMicrobiologyMicrobiologyGut communities03 medical and health sciencesHyles euphorbiaeEnterococcus casseliflavusEnterococcus sp.Original Researchmedia_commonmetagenomicsLarvabiologysecondary metabolitesgut communitiesSecondary metabolitesfungiBiofilmbiology.organism_classificationLepidoptera030104 developmental biology: lepidopteraMetagenomicsBacteriaFrontiers in Microbiology
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