Search results for "Sensitivity and specificity"

showing 10 items of 1080 documents

United States and European Multicenter Prospective Study for the Analytical Performance and Clinical Validation of a Novel Sensitive Fully Automated …

2016

Abstract BACKGROUND The objective of this study is the validation and proof of clinical relevance of a novel electrochemiluminescence immunoassay (ECLIA) for the determination of serum calcitonin (CT) in patients with medullary thyroid carcinoma (MTC) and in different diseases of the thyroid and of calcium homeostasis. METHODS This was a multicenter prospective study on basal serum CT concentrations performed in 9 US and European referral institutions. In addition, stimulated CT concentrations were measured in 50 healthy volunteers after intravenous calcium administration (2.5 mg/kg bodyweight). RESULTS In total, 1929 patients and healthy controls were included. Limits of blank, detection, …

AdultCalcitoninMalemedicine.medical_specialtyPercentileClinical Biochemistry030209 endocrinology & metabolismGastroenterologySensitivity and SpecificityThyroid carcinoma03 medical and health sciences0302 clinical medicineInternal medicinemedicineHumansClinical significanceProspective StudiesProspective cohort studyCalcium metabolismAutomation LaboratoryImmunoassaymedicine.diagnostic_testbusiness.industryBiochemistry (medical)ThyroidReproducibility of ResultsReference StandardsUnited StatesEuropeEndocrinologymedicine.anatomical_structureCalcitonin030220 oncology & carcinogenesisImmunoassayCalciumFemalebusinessClinical chemistry
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A randomized trial comparing conventional cytology to liquid-based cytology and computer assistance.

2012

Liquid-based cytology (LBC) has replaced conventional cytology (CC) for cervical cancer screening in some countries. However, it remains unclear whether LBC is superior to CC. A randomized controlled trial was conducted between August 2007 and March 2009 in Germany to compare LBC, alone and in combination with computer-assisted imaging technology (CAS), to CC in the detection of histologically confirmed cervical intraepithelial neoplasia (CIN). The main outcome measures were detection rates, relative sensitivities, positive predictive values (PPVs) and relative PPVs comparing LBC without and with CAS to CC. Primary histological outcome was CIN2 or higher. Included were 20,627 women particip…

AdultCancer Researchmedicine.medical_specialtyCytodiagnosisUterine Cervical NeoplasmsCervical intraepithelial neoplasiaGastroenterologySensitivity and Specificitylaw.inventionYoung AdultRandomized controlled triallawConventional cytologyCytologyInternal medicineMedicineHumansEarly Detection of CancerAgedGynecologyColposcopymedicine.diagnostic_testbusiness.industryReproducibility of ResultsMiddle Agedmedicine.diseaseConfidence intervalClinical trialOncologyLiquid-based cytologyFemalebusinessInternational journal of cancer
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Axillary Nodal Burden in Breast Cancer Patients With Pre-operative Fine Needle Aspiration-proven Positive Lymph Nodes Compared to Those With Positive…

2019

BACKGROUND/AIM: Recent years have seen a considerable shift to a more conservative management of the axilla in patients with positive axillary sentinel lymph nodes. The aim of this study was to determine whether some breast cancer patients with a preoperative ultrasound-guided needle aspiration biopsy proven positive node could potentially be spared an axillary lymph node dissection according to the ACOSOG Z0011 trial criteria. PATIENTS AND METHODS: A retrospective review was performed involving 623 breast cancer patients who underwent axillary lymph node dissection after either ultrasound-guided needle aspiration biopsy proven positive node or sentinel lymph node biopsy. RESULTS: Patients …

AdultCancer Researchmedicine.medical_specialtyaxillary nodal burdenCytodiagnosisSentinel lymph nodeBiopsy Fine-NeedleBreast NeoplasmsSensitivity and SpecificityGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciences0302 clinical medicineBreast cancerBreast cancerBiopsyPreoperative CareMedicineHumansBreastsentinel lymph node biopsyEndoscopic Ultrasound-Guided Fine Needle AspirationAgedRetrospective StudiesPharmacologyAged 80 and overmedicine.diagnostic_testbusiness.industryAxillary Lymph Node DissectionReproducibility of ResultsMiddle Agedmedicine.diseaseAxillaFine-needle aspirationmedicine.anatomical_structure030220 oncology & carcinogenesisLymphatic MetastasisAxillaFemaleRadiologyLymphLymph NodesbusinessNODALResearch Article
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Reproducibility of imaging human knee cartilage by delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) at 1.5 Tesla

2009

Summary Objective The purpose of this study was to investigate the day-to-day reproducibility of the delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) measurement at different knee joint surfaces in healthy subjects at 1.5 Tesla (T). Methods The dGEMRIC experiment was repeated for 10 asymptomatic volunteers three times with an average interval of 5 days between scans. The measurement was performed from a single sagittal slice through the center of the lateral femoral condyle and from the center of the patella in the axial plane. Cartilage was manually segmented into superficial, deep and full-thickness regions of interests (ROIs) at different topographical locations of the femur, tibia…

AdultCartilage ArticularGadolinium DTPAMalemusculoskeletal diseasesdGEMRICmedicine.medical_specialtyIntraclass correlationBiomedical EngineeringContrast MediaKnee JointSensitivity and Specificity030218 nuclear medicine & medical imaging03 medical and health sciences0302 clinical medicineRheumatologymedicineHumansFemurOrthopedics and Sports MedicineTibiaRadionuclide Imaging030222 orthopedicsReproducibilitybusiness.industryCartilageReproducibility of ResultsMiddle AgedImage Enhancementmusculoskeletal systemMagnetic Resonance ImagingReproducibilityKnee jointSagittal planeCartilagemedicine.anatomical_structureProteoglycanFemalePatellaRadiologybusinessNuclear medicineOsteoarthritis and Cartilage
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New spectrophotometric procedure for determining cefotaxime based on derivatization with 1,2-naphthoquinone-4-sulphonate into solid-phase extraction …

1998

Cefotaxime was derivatised with 1,2-naphthoquinone-4-sulphonate (NQS), extracted into solid-phase cartridges (C18) and detected using a UV-visible detection system. Optimum conditions for this new procedure were: hydrogencarbonate-carbonate buffer, pH 10.5, 5-min reaction time at 25 degrees C and an NQS concentration of 7.1x10(-3) mol l(-1). The accuracy and the precision of the liquid-solid procedure were tested. The procedure was used to measure cefotaxime in pharmaceutical and urine samples. The results obtained were contrasted with those reported for a HPLC method for urine samples. The generalized H-point standard additions method was used to measure cefotaxime in urine samples.

AdultCefotaximeChromatographyNQSGeneral ChemistryUrineCefotaximeHigh-performance liquid chromatographySensitivity and SpecificityCephalosporinschemistry.chemical_compoundchemistryPharmaceutical PreparationsSpectrophotometryStandard additionmedicineHumansIndicators and ReagentsSolid phase extractionDerivatizationChromatography High Pressure LiquidAntibacterial agentmedicine.drugNaphthoquinonesJournal of chromatography. B, Biomedical sciences and applications
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Does more favourable handling of the cerebrospinal fluid increase the diagnostic sensitivity of Borrelia burgdorferi sensu lato-specific PCR in Lyme …

2017

Tests for direct detection of Borrelia burgdorferi sensu lato (Bb) in Lyme neuroborreliosis (LNB) are needed. Detection of Bb DNA using PCR is promising, but clinical utility is hampered by low diagnostic sensitivity. We aimed to examine whether diagnostic sensitivity can be improved by the use of larger cerebrospinal fluid (CSF) volumes and faster handling of samples.Patients who underwent CSF examination for LNB were included. We collected two millilitres of CSF for PCR analysis, extracted DNA from the pellets within 24 h and analysed the eluate by two real-time PCR protocols (16S rRNA and OspA). Patients who fulfilled diagnostic criteria for LNB were classified as LNB cases and the rest …

AdultDNA BacterialMale0301 basic medicineMicrobiology (medical)030106 microbiologyPolymerase Chain ReactionSensitivity and SpecificitySpecimen Handlinglaw.inventionCohort Studies03 medical and health sciences0302 clinical medicineCerebrospinal fluidLyme diseaseBorrelia burgdorferi GrouplawAntibody negativeHumansLyme NeuroborreliosisMedicine030212 general & internal medicineBorrelia burgdorferiPcr analysisPolymerase chain reactionAgedAged 80 and overTick-borne diseaseGeneral Immunology and Microbiologybiologybusiness.industryGeneral MedicineMiddle Agedmedicine.diseasebiology.organism_classificationVirologyInfectious DiseasesLyme NeuroborreliosisImmunologyFemalebusinessInfectious Diseases
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Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutrope…

2011

Summary Objectives The objectives of this study were to compare the performance of the LightCycler SeptiFast Test MGRADE and conventional blood culture in the etiological diagnosis of febrile episodes occurring in neutropenic and critically ill patients (in the intensive care unit; ICU), and to assess the potential clinical value of the SeptiFast test in patient management. Methods A total of 86 febrile episodes occurring in 33 neutropenic patients and 53 ICU patients were analyzed. Blood samples for blood culture and SeptiFast testing were obtained at the onset of fever, before the implementation of empirical antimicrobial therapy. Results The overall microorganism-to-isolate agreement bet…

AdultDNA BacterialMaleMicrobiology (medical)medicine.medical_specialtyNeutropeniaFevermedicine.drug_classCritical IllnessAntibioticsBacteremiaBloodstream infectionNeutropeniaCommunicable DiseasesPolymerase Chain ReactionSensitivity and SpecificityBlood culturelaw.inventionCohort StudiesCritically ill patientslawmedicineHumansBlood cultureDiagnostic accuracy of the SeptiFast assayDNA FungalIntensive care medicineFungemiaAgedRetrospective StudiesAged 80 and overmedicine.diagnostic_testbusiness.industryCritically illGeneral MedicineMiddle Agedmedicine.diseaseIntensive care unitIntensive Care UnitsInfectious DiseasesMolecular Diagnostic TechniquesSpainBacteremiaEtiologyFemaleReagent Kits DiagnosticbusinessFungemiaReal-time PCRInternational Journal of Infectious Diseases
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Enhancement pattern of small hepatocellular carcinoma (HCC) at contrast-enhanced US (CEUS), MDCT, and MRI: intermodality agreement and comparison of …

2012

OBJECTIVE:To evaluate agreement between contrast-enhanced ultrasound (CEUS), multi-detector row computed tomography (MDCT) and magnetic resonance imaging (MRI) for the assessment of typical and atypical enhancement patterns of small hepatocellular carcinoma (HCC); and to compare diagnostic sensitivity of 2005 and 2010 American Association for the Study of Liver Diseases (AASLD) guidelines. MATERIALS AND METHODS: Between January 2008 and December 2009, we included cirrhotic patients with newly diagnosed 10-20 mm HCC imaged at two contrast-enhanced imaging techniques among CEUS, MDCT, and MRI. Dynamic studies were reviewed by two radiologists to assess enhancement pattern. Percentage of cases…

AdultDiagnostic ImagingMalemedicine.medical_specialtyCarcinoma HepatocellularContrast MediaEnhancement patternSensitivity and SpecificityImaging modalitiesTumor enhancementMcNemar's testmedicineHumansRadiology Nuclear Medicine and imagingAgedAged 80 and overmedicine.diagnostic_testbusiness.industryLiver NeoplasmsUltrasoundReproducibility of ResultsMagnetic resonance imagingGeneral MedicineMiddle Agedmedicine.diseaseUnited Statessmall hepatocellular carcinomaItalyHepatocellular carcinomaPractice Guidelines as TopicFemaleRadiologybusinesscontrast-enhanced ultrasoundContrast-enhanced ultrasound
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Fecal Calprotectin in Clinical Practice

2012

Background: Surrogate markers of colorectal inflammation are increasingly being recognized as important in differentiating organic from functional intestinal disorders. Fecal calprotectin (FC) can be easily measured in the stool, being released by leukocytes in inflammatory conditions. Aim: We evaluated FC as an index of inflammation in consecutive outpatients referred for colonoscopy for chronic, nonbloody diarrhea. Methods: Stool specimens of 346 outpatients with chronic, nonbloody diarrhea, referred for colonoscopy, were measured for FC levels. The proportion of patients correctly diagnosed with the test and the relationship with endoscopic and histologic findings were measured. Results:…

AdultDiarrheaMalemedicine.medical_specialtySettore MED/09 - Medicina InternaAdolescentdiarrheaColonoscopyInflammationSettore MED/08 - Anatomia PatologicaSensitivity and SpecificityGastroenterologyFecesYoung AdultPredictive Value of TestsInternal medicinemedicineHumansScreening toolProspective StudiesFecesAgedAged 80 and overInflammationSettore MED/12 - Gastroenterologiamedicine.diagnostic_testbusiness.industryGastroenterologyHistologyColonoscopyMiddle AgedEndoscopyDiarrheaChronic DiseaseFemalemedicine.symptomCalprotectinbusinessLeukocyte L1 Antigen ComplexJournal of Clinical Gastroenterology
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First-trimester screening for trisomy-21 using a simplified method to assess the presence or absence of the fetal nasal bone.

2005

Objective To determine the benefit of including nasal bone assessment in addition to standard first-trimester markers (nuchal translucency, free beta human chorionic gonadotropin and pregnancy-associated plasma protein A) as a screening test for Down syndrome, using a strict criterion for classification of nasal bone absence. Study design Nasal bone assessment was conducted in 2411 patients with crown-rump length between 45 and 84 mm, including 15 patients with Down syndrome. A patient was considered to have an absent nasal bone only if there was no evidence of present nasal bone. Unlike other studies, nasal bone was classified as present when there was evidence of a thin echogenic line und…

AdultDown syndromemedicine.medical_specialtyPregnancy-associated plasma protein AUrologyAneuploidyPrenatal diagnosisEmbryonic StructuresSensitivity and SpecificityCrown-Rump LengthUltrasonography PrenatalPredictive Value of TestsPregnancyReference ValuesRisk FactorsmedicineConfidence IntervalsHumansMass ScreeningNasal BoneProbabilityObstetricsbusiness.industryObstetrics and GynecologyEchogenicityPrenatal Caremedicine.diseaseNasal boneConfidence intervalPregnancy Trimester FirstCase-Control StudiesLinear ModelsFemaleDown SyndromeTrisomybusinessNuchal Translucency MeasurementAmerican journal of obstetrics and gynecology
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