Search results for "Sensor"
showing 10 items of 4594 documents
Behavioral evaluation of aging in experimental animals
2021
Abstract Since aging is a very a complex multisystemic process, behavioral assessment must cover a combined panoply of physical and neural functions. Behavioral measures must be objective and reproducible to be able to compare between individuals of different ages or experimental conditions. In this chapter, we discuss how aging is reflected in a very variable way in different physical, cognitive, emotional, social, motor, and sensory behaviors. We suggest specific tests to evaluate each of these functions in mice, discussing previous results obtained in different genetic strains. Finally, we identify the most distorting variables to consider, such as age range, gender, or body weight, to m…
Early stage diagnosis of programmed cell death (apoptosis) using electroanalysis: Nanomaterial and methods overview
2017
Timely monitoring of apoptosis can effectively assist early diagnosis of related diseases and continuous evaluation of the efficacy of drugs. Therefore, developing advanced methods for apoptosis evaluation has attracted more and more attention. In addition, the increased demand for understanding the early stages of apoptosis is pushing the envelope for solutions in early instance real-time monitoring of death kinetics. In this review, various types of electro-analytical methods for detection of apoptosis were discussed. The emphasis is placed in diagnosis of apoptosis based on cyto-, immune-, geno-, apta-, and peptide based biosensing strategies. In the first part of review, various electro…
Docosahexaenoic acid protects human RPE cells against oxidative stress via PI3K/Akt m-TOR/p70-p85S6K pathways
2012
Purpose Oxidative Stress (OS) plays a critical role in the pathogenesis of age-related macular degeneration (AMD), especially by targeting the retinal pigment epithelium (RPE). Dietary habits with high consumption of docosahexaenoic acid (DHA) have been shown to prevent the development and evolution of AMD. Nevertheless, it is still unclear how DHA affects AMD. Our study aimed to investigate the involvement of the PI3K/Akt and m-TOR/p70-p85S6K pathways in human RPE cells after induction of OS, and then to assess the effect of DHA in the signaling pathways and in the protection against RPE cell death. Methods For this purpose, we used ARPE-19 cells exposed to the prooxidant agent, tert-butyl…
Step-along power vector method for astigmatic wavefront propagation
2013
Purpose To propose both a new algebraic solution and a graphical monitoring method for astigmatic wavefront propagation in the framework provided by power vectors. Methods The generalised propagation equation describing the propagation of astigmatic wavefronts from one plane to another is adapted to the power vectors formalism using a novel algorithm based on a step-along method. The step-along procedure is directly applied to the tuple of power vectors [M, J0, J45] representing an arbitrary astigmatic wavefront and it permits the calculation of the tuple of power vectors [M′, J′0, J′45] after a given propagation distance. This is achieved mathematically first by temporarily rotating the as…
Systematic review of chewing simulators : reality and reproducibility of in vitro studies
2020
Background The aim of this systematic review was to analyze the types of human chewing simulator described in scientific literature. Material and Methods An electronic search was conducted in the databases PubMed, Embase and Scopus. The search strategy included 10 search terms: “in vitro”; “dental materials”; “shear strength”; “fatigue fracture”; “bite force”; “prosthetic materials”; “chewing simulator”; “chewing machine”; “simulated mastication”; and “dental wear simulator.” Two researchers worked independently to assess the titles and abstracts of the articles. The quality of the in vitro trials selected was evaluated by means of the Consolidated Standards of Reporting Trials scale. Resul…
A Synthetic Route to 3-(Heteroaryl)-7-hydroxycoumarins Designed for Biosensing Applications
2014
A straightforward method to synthesize 3-(2-benzimidazolyl)-7-hydroxycoumarins, based on the condensation reaction of 7-acetoxy-3-formylcoumarin with various C- and/or N-substituted ortho-phenylenediamine derivatives is presented. This unusual approach proved particularly effective for introducing different hydrophilic groups (carboxylic or sulfonic acids or trimethylalkylammonium moieties) onto the heteroaryl scaffold, leading to cyan-green emitting coumarins that were both water-soluble and strongly fluorescent under physiological conditions. The further extension of this condensation reaction to bis(2-aminophenyl)diselenide enabled the first synthesis of 3-(2-benzoselenazolyl)-7-hydroxyc…
Insights into functional aspects of centrins from the structure of N-terminally extended mouse centrin 1
2006
AbstractCentrins are members of the family of Ca2+-binding EF-hand proteins. In photoreceptor cells, centrin isoform 1 is specifically localized in the non-motile cilium. This connecting cilium links the light-sensitive outer segment with the biosynthetic active inner segment of the photoreceptor cell. All intracellular exchanges between these compartments have to occur through this cilium. Three-dimensional structures of centrins from diverse organisms are known, showing that the EF-hand motifs of the N-terminal domains adopt closed conformations, while the C-terminal EF-hand motifs have open conformations. The crystal structure of an N-terminally extended mouse centrin 1 (MmCen1-L) resemb…
Fast Photochemistry of Prototypical Phytochromes—A Species vs. Subunit Specific Comparison
2015
Phytochromes are multi-domain red light photosensor proteins, which convert red light photons to biological activity utilizing the multitude of structural and chemical reactions. The steady increase in structural information obtained from various bacteriophytochromes has increased understanding about the functional mechanism of the photochemical processes of the phytochromes. Furthermore, a number of spectroscopic studies have revealed kinetic information about the light-induced reactions. The spectroscopic changes are, however, challenging to connect with the structural changes of the chromophore and the protein environment, as the excited state properties of the chromophores are very sens…
An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
2016
Cellular organelles provide opportunities to relate biological mechanisms to disease. Here we use affinity proteomics, genetics and cell biology to interrogate cilia: poorly understood organelles, where defects cause genetic diseases. Two hundred and seventeen tagged human ciliary proteins create a final landscape of 1,319 proteins, 4,905 interactions and 52 complexes. Reverse tagging, repetition of purifications and statistical analyses, produce a high-resolution network that reveals organelle-specific interactions and complexes not apparent in larger studies, and links vesicle transport, the cytoskeleton, signalling and ubiquitination to ciliary signalling and proteostasis. We observe sub…
Syntaxin13 expression is regulated by mammalian target of rapamycin (mTOR) in injured neurons to promote axon regeneration.
2014
Injured peripheral neurons successfully activate intrinsic signaling pathways to enable axon regeneration. We have previously shown that dorsal root ganglia (DRG) neurons activate the mammalian target of rapamycin (mTOR) pathway following injury and that this activity enhances their axon growth capacity. mTOR plays a critical role in protein synthesis, but the mTOR-dependent proteins enhancing the regenerative capacity of DRG neurons remain unknown. To identify proteins whose expression is regulated by injury in an mTOR-dependent manner, we analyzed the protein composition of DRGs from mice in which we genetically activated mTOR and from mice with or without a prior nerve injury. Quantitati…