Search results for "Sequence analysis"

showing 10 items of 1349 documents

EST based phylogenomics of Syndermata questions monophyly of Eurotatoria

2008

Abstract Background The metazoan taxon Syndermata comprising Rotifera (in the classical sense of Monogononta+Bdelloidea+Seisonidea) and Acanthocephala has raised several hypotheses connected to the phylogeny of these animal groups and the included subtaxa. While the monophyletic origin of Syndermata and Acanthocephala is well established based on morphological and molecular data, the phylogenetic position of Syndermata within Spiralia, the monophyletic origin of Monogononta, Bdelloidea, and Seisonidea and the acanthocephalan sister group are still a matter of debate. The comparison of the alternative hypotheses suggests that testing the phylogenetic validity of Eurotatoria (Monogononta+Bdel…

0106 biological sciencesCharacter evolutionEvolutionRotiferaZoologyBiology010603 evolutionary biology01 natural sciencesAcanthocephalaEvolution Molecular03 medical and health sciencesMonophylyPhylogeneticsHelminthsQH359-425RNA Ribosomal 18SAnimalsBdelloideaPhylogenyEcology Evolution Behavior and Systematics030304 developmental biologyExpressed Sequence Tags0303 health sciencesPhylogenetic treeSyndermata; phylogenetic positionGenomicsSequence Analysis DNADNA Helminthbiology.organism_classificationGenetics PopulationSister groupPlatyhelminthsEvolutionary biologyMolecular phylogeneticsAcanthocephalaResearch ArticleBMC Evolutionary Biology
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Changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves.

2011

International audience; The oomycete Plasmopara viticola is responsible for downy mildew, a severe grapevine disease. In infected grapevine leaves, we have observed an abnormal starch accumulation at the end of the dark period, suggesting modifications in starch metabolism. Therefore, several complementary approaches, including transcriptomic analyses, measurements of enzyme activities, and sugar quantification, were performed in order to investigate and to understand the effects of P. viticola infection on leaf starch and-to a larger extent-carbohydrate metabolism. Our results indicate that starch accumulation is associated with an increase in ADP-glucose pyrophosphorylase (AGPase) activit…

0106 biological sciencesChlorophyllPhysiologyStarchenzymatic activityhexosesbeta-AmylaseplantGlucose-1-Phosphate Adenylyltransferasetranscriptomic analyse01 natural sciencesinvertasechemistry.chemical_compoundphytopathogenGene Expression Regulation PlantVitisTrehalaseOligonucleotide Array Sequence Analysis0303 health sciencesbiologyfood and beveragesStarchGeneral MedicineEnzymesBiochemistryOomycetesRNA PlantPlasmopara viticolaCarbohydrate metabolism03 medical and health sciencesPlasmopara viticolaADP-glucose pyrophosphorylasePolysaccharidesVignecarbohydrate metabolism[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biologytrehalose030304 developmental biologyPlant Diseasesphotosynthesisbiology.organism_classificationtrehalaseTrehaloseEnzyme assayPlant LeavesInvertasechemistryVitis viniferabiology.proteinDowny mildewfungialpha-AmylasesphysiopathologyAgronomy and Crop Science010606 plant biology & botany
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The genome sequencing of an albino Western lowland gorilla reveals inbreeding in the wild

2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.-- et al.

0106 biological sciencesConservation geneticsMalegenotype phenotype correlationGorillaComputingMilieux_LEGALASPECTSOFCOMPUTINGarginineGenoma humà01 natural sciencesOculocutaneous albinism type 4single nucleotide polymorphismAlbinismegenetic variabilityGorillaInbreedinggenetic conservationGenetics0303 health sciencesGenomebiologyarticlecopy number variationHigh-Throughput Nucleotide SequencingSLC45A2 geneGenomicszygosityOculocutaneous albinismFloquet de neu (Goril·la)AlbinismFemaleBiotechnologyamino acid substitutionResearch ArticleSLC45A2Gorilla gorilla gorillaHeterozygoteAlbinismMolecular Sequence Datacomparative genomic hybridizationgene sequenceConservation010603 evolutionary biology03 medical and health sciencesWestern lowland gorillabiology.animalmedicineGeneticsheterozygosityAnimalsAmino Acid Sequencegene030304 developmental biologygene identificationWhole genome sequencingnonhumanGorilla gorillaMembrane Transport ProteinsSequence Analysis DNA15. Life on landbiology.organism_classificationmedicine.diseaseGenòmicaData_GENERALMutationbiology.proteinGenèticaoculocutaneous albinismglycineMicrosatellite RepeatsBMC Genomics
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Pleurotus opuntiae revisited e An insight to the phylogeny of dimitic Pleurotus species with emphasis on the P. djamor complex

2018

Abstract The name Pleurotus opuntiae is indiscriminately used for describing mushrooms with white to off-white to white-grey pilei with short or absent stipe and dimitic hyphal system, which grow on plants of the genera Opuntia, Yucca, Agave, Phytolacca etc. However, the outcome of the present study evidences that this name should be reserved for specimens deriving from the Mediterranean area only; an epitype originating from Italy on Opuntia ficus-indica is designated. Pertinent material was sequenced by using the internal transcribed spacer region (ITS) and found to be phylogenetically related to P. djamor from Kenya and Nigeria, while members of the P. djamor complex from other continent…

0106 biological sciencesContext (language use)Pleurotus01 natural sciences03 medical and health sciencesStipe (botany)BotanyDNA Ribosomal SpacerRNA Ribosomal 28SGeneticsCluster AnalysisInternal transcribed spacerDNA FungalRibosomal DNAEcology Evolution Behavior and SystematicsPhylogeny030304 developmental biology0303 health sciencesPleurotusbiologyMediterranean RegionSettore BIO/02 - Botanica SistematicaSequence Analysis DNAPlantsbiology.organism_classificationAgaveInfectious DiseasesTaxonGenetic distanceItalySettore BIO/03 - Botanica Ambientale E ApplicataRNA Polymerase IIFungal taxonomy ITS Multi-gene phylogeny Opuntia ficus-indica Oyster mushroom Pleurotus opuntiae epitype010606 plant biology & botany
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Toward reconstructing the evolution of advanced moths and butterflies (Lepidoptera: Ditrysia): an initial molecular study

2009

AbstractBackgroundIn the mega-diverse insect order Lepidoptera (butterflies and moths; 165,000 described species), deeper relationships are little understood within the clade Ditrysia, to which 98% of the species belong. To begin addressing this problem, we tested the ability of five protein-coding nuclear genes (6.7 kb total), and character subsets therein, to resolve relationships among 123 species representing 27 (of 33) superfamilies and 55 (of 100) families of Ditrysia under maximum likelihood analysis.ResultsOur trees show broad concordance with previous morphological hypotheses of ditrysian phylogeny, although most relationships among superfamilies are weakly supported. There are als…

0106 biological sciencesEntomologyNuclear geneUNESCO::CIENCIAS DE LA VIDA::Biología animal (Zoología) ::InvertebradosEvolutionmedia_common.quotation_subjectInitial molecular studyZoologyInsect010603 evolutionary biology01 natural sciencesProtein-coding nuclear genesLepidoptera genitaliaLepidoptera; Protein-coding nuclear genes; Initial molecular study03 medical and health sciencesDitrysiaPhylogenetics:CIENCIAS DE LA VIDA::Biología animal (Zoología) ::Invertebrados [UNESCO]Research articleQH359-425AnimalsCladeEcology Evolution Behavior and SystematicsPhylogeny030304 developmental biologymedia_common0303 health sciencesbiologyBayes TheoremSequence Analysis DNAbiology.organism_classificationBiological EvolutionLepidopteraEvolutionary biologyBombycoideaBMC Evolutionary Biology
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Expression of xyloglucan endotransglucosylase/hydrolase (XTH) genes and XET activity in ethylene treated apple and tomato fruits.

2013

[EN] Xyloglucan endotransglucosylase/hydrolase (XTHs: EC 2.4.1.207 and/or EC 3.2.1.151), a xyloglucan modifying enzyme, has been proposed to have a role during tomato and apple fruit ripening by loosening the cell wall. Since the ripening of climacteric fruits is controlled by endogenous ethylene biosynthesis, we wanted to study whether XET activity was ethylene-regulated, and if so, which specific genes encoding ripening-regulated XTH genes were indeed ethylene-regulated. XET specific activity in tomato and apple fruits was significantly increased by the ethylene treatment, as compared with the control fruits, suggesting an increase in the XTH gene expression induced by ethylene. The 25 Sl…

0106 biological sciencesEthylenePhysiologyPlant ScienceBiologyTransglucosylation and xyloglucan01 natural sciencesCell wall03 medical and health scienceschemistry.chemical_compoundSolanum lycopersicumPlant Growth RegulatorsGene Expression Regulation PlantGene expressionBIOQUIMICA Y BIOLOGIA MOLECULARGenePhylogeny030304 developmental biology2. Zero hunger0303 health sciencesSequence Homology Amino AcidCell wallAgriculturaGlycosyltransferasesfood and beveragesRipeningSequence Analysis DNAXyloglucan endotransglucosylaseEthylenesFruit ripeningXyloglucanMalus domesticachemistryBiochemistryFruitMalusClimactericAgronomy and Crop Science010606 plant biology & botany
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Phylogeography and Molecular Evolution of Potato virus Y

2012

Potato virus Y (PVY) is an important plant pathogen, whose host range includes economically important crops such as potato, tobacco, tomato, and pepper. PVY presents three main strains (PVYO, PVYN and PVYC) and several recombinant forms. PVY has a worldwide distribution, yet the mechanisms that promote and maintain its population structure and genetic diversity are still unclear. In this study, we used a pool of 77 complete PVY genomes from isolates collected worldwide. After removing the effect of recombination in our data set, we used Bayesian techniques to study the influence of geography and host species in both PVY population structure and dynamics. We have also performed selection and…

0106 biological sciencesEvolutionary GeneticsAmino-acid sitesSelective constraintsPotyviruslcsh:Medicine01 natural sciencesAmino-Acid SitesRecombinant strainPlant RNA virusesNegative selectionMaximum-Likelihoodlcsh:Sciencepathologie végétaleSelective ConstraintsPhylogenyGenetics0303 health sciencesCoat proteinMultidisciplinaryNatural selectionVegetal BiologybiologyEcologyGenetic-structurePotyvirusfood and beveragesEuropePhylogeneticsVenous necrosisPhylogeographyPotato virus YBiogeographyVenous NecrosisSequence AnalysisResearch ArticlePlant RNA VirusesGenome ViralMicrobiologyEvolution Molecular03 medical and health sciencesGenetic-StructureMolecular evolutionVirologyMosaic-virus[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyEvolutionary SystematicsBiology030304 developmental biologySolanum tuberosumGenetic diversityEvolutionary BiologyMosaic virusHost (biology)Maximum-likelihoodlcsh:RComputational Biologyvirus à de la pomme de terreBayes Theoremlégumebiology.organism_classificationMutational analysisMosaic-VirusMutational AnalysisEvolutionary EcologyRecombinant StrainNorth Americalcsh:QBiologie végétalePopulation Genetics010606 plant biology & botany
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Evidence of a highly complex phylogeographic structure on a specialist river bird species, the dipper (Cinclus cinclus)

2008

This study details the phylogeographic pattern of the white-throated dipper (Cinclus cinclus), a Palearctic, temperate, passerine bird that is exclusively associated with flowing water. Our results reveal a complex phylogeographic structure with at least five distinct lineages for the Western Palearctic region. As for many species of the Western Palearctic fauna and flora, this genetic structure is probably linked to the isolation of populations in different southern refuges during glacial periods. Furthermore, the isolation of populations in Scandinavia and/or Eastern regions, but also in Morocco and probably in Corsica, was accentuated by ecological and biogeographic barriers during Quate…

0106 biological sciencesFauna[SDV]Life Sciences [q-bio]Settore BIO/05 - ZoologiaSubspecies01 natural sciencesPHYLOGEOGRAPHYIce ageGlacial periodPasseriformesPhylogenyComputingMilieux_MISCELLANEOUS0303 health scienceseducation.field_of_studyLikelihood FunctionsGLACIAL REFUGEbiologyGeographyEcologyGLACIATION QUATERNAIRECytochromes bMitochondriaEuropeGenes MitochondrialInterglacialCinclus cinclus; Glacial refuges; Mitochondrial DNA; Phylogeography; Quaternary glaciations; TaxonomyMITOCHONDRIAL DNAGenetic MarkersCinclus cincluPopulation010603 evolutionary biologyDNA MitochondrialEvolution Molecular03 medical and health sciencesGeneticsAnimalseducationMolecular BiologyEcology Evolution Behavior and Systematics030304 developmental biology[SDV.GEN]Life Sciences [q-bio]/GeneticsGenetic VariationSequence Analysis DNA15. Life on landTAXONOMYbiology.organism_classificationPhylogeographyGenetics PopulationCINCLUS CINCLUSCinclus cinclusQUATERNARY GLACIATIONSequence Alignment
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An optimised multi-host trematode life cycle: fishery discards enhance trophic parasite transmission to scavenging birds

2016

Overlapping distributions of hosts and parasites are critical for successful completion of multi-host parasite life cycles and even small environmental changes can impact on the parasite's presence in a host or habitat. The generalist Cardiocephaloides longicollis was used as a model for multi-host trematode life cycles in marine habitats. This parasite was studied to quantify parasite dispersion and transmission dynamics, effects of biological changes and anthropogenic impacts on life cycle completion. We compiled the largest host dataset to date, by analysing 3351 molluscs (24 species), 2108 fish (25 species) and 154 birds (17 species) and analysed the resultant data based on a number of …

0106 biological sciencesFood ChainFish farmingSnailsFishingFisheriesTrematode InfectionsBiologyDNA Ribosomal010603 evolutionary biology01 natural sciencesHost SpecificityBirdsFish DiseasesAquacultureRNA Ribosomal 28SMediterranean SeaAnimalsBody SizeHuman ActivitiesCardiocephaloides longicollisEcosystemTrophic levelLife Cycle StagesBird Diseasesbusiness.industryEcology010604 marine biology & hydrobiologyFishesMarine habitatsIntermediate hostSequence Analysis DNADiscardsFisheryInfectious DiseasesBlack SeaMolluscaParasitologyTrematodabusinessInternational Journal for Parasitology
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Interspecific interactions influence contrasting spatial genetic structures in two closely related damselfly species

2014

Spatial genetic structure (SGS) is largely determined by colonization history, landscape and ecological characteristics of the species. Therefore, sympatric and ecologically similar species are expected to exhibit similar SGSs, potentially enabling prediction of the SGS of one species from that of another. On the other hand, due to interspecific interactions, ecologically similar species could have different SGSs. We explored the SGSs of the closely related Calopteryx splendens and Calopteryx virgo within Finland and related the genetic patterns to characteristics of the sampling localities. We observed different SGSs for the two species. Genetic differentiation even within short distances …

0106 biological sciencesGene FlowInsectaRange (biology)Population geneticsBiology010603 evolutionary biology01 natural sciencesGene flow03 medical and health sciencesGenetic driftSpecies SpecificityGeneticsAnimalsCluster AnalysisEcology Evolution Behavior and SystematicsFinland030304 developmental biology0303 health sciencesGenetic diversityGeographyEcologyGenetic DriftGenetic VariationBayes TheoremInterspecific competitionSequence Analysis DNA15. Life on landSympatryGenetics PopulationSympatric speciationGenetic structureta1181Molecular Ecology
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