Search results for "Sequence"
showing 10 items of 4987 documents
A note on discrete sets
2009
We give several partial positive answers to a question of Juhasz and Szentmiklossy regarding the minimum number of discrete sets required to cover a compact space. We study the relationship between the size of discrete sets, free sequences and their closures with the cardinality of a Hausdorff space, improving known results in the literature.
Investigation and control of a Norovirus outbreak of probable waterborne transmission through a municipal groundwater system
2014
During March 2011 an outbreak of gastroenteritis occurred in Santo Stefano di Quisquina, Agrigento, Sicily, Italy. Within two weeks 156 cases were identified among the 4,965 people living in the municipality. An epidemiological investigation was conducted to characterize the outbreak and target the control measures. A case was defined as a person developing diarrhea or vomiting during February 27–March 13, 2011. Stool specimens were collected from 12 cases. Norovirus (NoV) genotype GII.4 variant New Orleans 2009 was identified in stool samples from 11 of 12 cases tested (91.7%). Epidemiological investigations suggested a possible association with municipal drinking water consumption. Water …
Consistent phenological shifts in the making of a biodiversity hotspot: the Cape flora
2011
Abstract Background The best documented survival responses of organisms to past climate change on short (glacial-interglacial) timescales are distributional shifts. Despite ample evidence on such timescales for local adaptations of populations at specific sites, the long-term impacts of such changes on evolutionary significant units in response to past climatic change have been little documented. Here we use phylogenies to reconstruct changes in distribution and flowering ecology of the Cape flora - South Africa's biodiversity hotspot - through a period of past (Neogene and Quaternary) changes in the seasonality of rainfall over a timescale of several million years. Results Forty-three dist…
Los beneficios del mar: negocios e inversiones económicas de la oficialidad naval en el siglo XVIII
2019
Throughout the eighteenth century, the Spanish navy was extensively reformed and enhanced as a consequence of the new model of Monarchy that the Bourbons introduced. Among the measures adopted, it is remarkable the erection of the naval officers corps according to the needs and obligations that maritime war had in this century. This group becomes the paradigm of the new professionalization and new training approach, and its members had great relevance due to its great mobility around the empire and the power of the naval institution. This means that a part of the naval officers were placed in privileged positions and at the intersection of diverse political, social and economic networks. Th…
Design and experimental validation of a generic model for combinatorial assembly of DNA tiles into 1D-structures.
2010
Abstract Background Quantitative modeling of the self-assembly of DNA tiles leading either to defined end-products or distribution of biopolymers is of practical importance for biotechnology and synthetic biology. Methods The combinatorial process describing tile assembly was implemented into a generic algorithm allowing quantitative description of the population of significant species accumulating during the reaction course. Experimental formation and characterization by optical and electrophoresis approaches of copolymers resulting from the self-assembly of a limited number of half-complementary tiles were used to define and validate generic rules allowing definition of model parameters. …
Diferencias entre empleabilidad, inseguridad laboral y salud en trabajadores y desempleados
2018
ResumenEl desempleo y la inseguridad laboral tienen efectos negativos sobre la salud, aunque la empleabilidad podría mediar dichos efectos. El presente artículo tiene como objetivo analizar las posibles diferencias entre trabajadores y desempleados en empleabilidad y variables de salud psicosocial, así como explorar el papel de la empleabilidad y la inseguridad laboral en la salud psicosocial. Para esto, se utilizó metodología cuasiexperimental, donde se administraron una serie de cuestionarios que evaluaban empleabilidad e inseguridad laboral a 97 trabajadores (35 desempleados y 62 en activo), junto a otras variables psicosociales y de salud. Los resultados muestran que la empleabilidad es…
Parsimonious scenario for the emergence of viroid-like repliconsde novo
2019
AbstractViroids are small, non-coding, circular RNA molecules that infect plants. Different hypotheses for their evolutionary origin have been put forward, such as an early emergence in a precellular RNA World or severalde novoindependent evolutionary origins in plants. Here we discuss the plausibility ofde novoemergence of viroid-like replicons by giving theoretical support to the likelihood of different steps along a parsimonious evolutionary pathway. While Avsunviroidae-like structures are relatively easy to obtain through evolution of a population of random RNA sequences of fixed length, rod-like structures typical of Pospiviroidae are difficult to fix. Using different quantitative appr…
The endopolygalacturonase 1 from botrytis cinerea activates grapevine defense reactions unrelated to its enzyumatic activity
2003
A purified glycoprotein from Botrytis cinerea(strain T4), identified as endopolygalacturonase 1 (T4BcPG1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (Vitis vinifera cv. Gamay). These reactions include calcium influx, production of active oxygen species, activation of two mitogen-activated protein kinases, defense gene transcript accumulation, and phytoalexin production. Most of these defense reactions were also activated in grapevine in response to purified oligogalacturonides (OGA) with a degree of polymerization of 9 to 20. In vivo, these active OGA might be a part of the released products resulting from endopolygalacturonase activity on plan…
Identification and characterization of autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis
1998
SUMMARY Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease of unknown aetiology. Recent studies have shown that genetic factors and both cellular and humoral immunological abnormalities are important in the pathogenesis of PSC. The most prominent autoantibodies in PSC are anti-neutrophil cytoplasmic antibodies (ANCA). The autoepitopes of ANCA in PSC are not well defined. The aim of this study was to identify corresponding ANCA autoantigens in patients with PSC. A biochemical approach with enrichment and partial purification of soluble neutrophil proteins, detection of autoantibodies by Western blot and partial amino acid sequencing were used. Two new autoantigen/aut…
De novo CCND2 mutations leading to stabilization of cyclin D2 cause megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome
2014
Activating mutations in genes encoding phosphatidylinositol 3-kinase (PI3K)-AKT pathway components cause megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome (MPPH, OMIM 603387)(1-3). Here we report that individuals with MPPH lacking upstream PI3K-AKT pathway mutations carry de novo mutations in CCND2 (encoding cyclin D2) that are clustered around a residue that can be phosphorylated by glycogen synthase kinase 313 (GSK-3 beta)(4). Mutant CCND2 was resistant to proteasomal degradation in vitro compared to wild-type CCND2. The PI3K-AKT pathway modulates GSK-3 beta activity(4), and cells from individuals with PIK3CA, PIK3R2 or AKT3 mutations showed similar CCND2 accumulation. CCND…