6533b837fe1ef96bd12a2a24
RESEARCH PRODUCT
The endopolygalacturonase 1 from botrytis cinerea activates grapevine defense reactions unrelated to its enzyumatic activity
Yves BrygooJérome GarinElodie VandelleCaroline LevisMarc BentéjacAlain PuginBenoît PoinssotMarielle AdrianFrancesca SiciliaPierre Coutos-thévenotsubject
elicitor; endopolygalacturonase 1; Botrytis cinerea; plant defenceplant defencePhysiologyMolecular Sequence DataOligosaccharidesBiologyCell wallBotrytis cinereaGene Expression Regulation PlantSequence Homology Nucleic Acid[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyVitisAmino Acid SequencePectinaseendopolygalacturonase 1ComputingMilieux_MISCELLANEOUSBotrytis cinereachemistry.chemical_classificationelicitorBase SequenceSequence Homology Amino AcidKinasePhytoalexinfood and beveragesGeneral MedicineHydrogen Peroxidebiology.organism_classificationImmunity InnateElicitorEnzyme ActivationEnzymePolygalacturonasechemistryBiochemistryCalciumBotrytisMitogen-Activated Protein KinasesGlycoproteinAgronomy and Crop Sciencedescription
A purified glycoprotein from Botrytis cinerea(strain T4), identified as endopolygalacturonase 1 (T4BcPG1) by mass spectrometry analysis, has been shown to activate defense reactions in grapevine (Vitis vinifera cv. Gamay). These reactions include calcium influx, production of active oxygen species, activation of two mitogen-activated protein kinases, defense gene transcript accumulation, and phytoalexin production. Most of these defense reactions were also activated in grapevine in response to purified oligogalacturonides (OGA) with a degree of polymerization of 9 to 20. In vivo, these active OGA might be a part of the released products resulting from endopolygalacturonase activity on plant cell walls. Nevertheless, the intensity and kinetics of events triggered by OGA were very different when compared with T4BcPG1 effects. Moreover, chemical treatments of T4BcPG1 and desensitization assays have allowed us to discriminate enzymatic and elicitor activities, indicating that elicitor activity was not due to released oligogalacturonides. Thus, BcPG1 should be considered as both an avirulence and a virulence factor. The role of the secreted BcPG1 in the pathogenicity of Botrytis cinerea is discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-01-01 |