Search results for "Shock"

Laser shock processing with two different laser sources on 2050‐T8 aluminum alloy

2011

PurposeThe purpose of this paper is to conduct a comparative study of the surface modifications induced by two different lasers on a 2050‐T8 aluminum alloy, with a specific consideration of residual stress and work‐hardening levels.Design/methodology/approachTwo lasers have been used for Laser shock peening (LSP) treatment in water‐confined regime: a Continuum Powerlite Plus laser, operating at 0.532 mm with 9 ns laser pulses, and near 1.5mm spot diameters; a new generation Gaia‐R Thales laser delivering 10 J‐10 ns impacts, with 4‐6mm homogeneous laser spots at 1.06 mm. Surface deformation, work‐hardening levels and residual stresses were analyzed for both LSP conditions. Residual stresses …

Membrane Stiffness of Animal Cells Challenged by Osmotic Stress

2006

Quaternary structures of GroEL and naïve-Hsp60 chaperonins in solution: a combined SAXS-MD study

2015

The quaternary structures of bacterial GroEL and human naïve-Hsp60 chaperonins in physiological conditions have been investigated by an innovative approach based on a combination of synchrotron Small Angle X-ray Scattering (SAXS) in-solution experiments and molecular dynamics (MD) simulations. Low-resolution SAXS experiments over large and highly symmetric oligomers are analyzed on the basis of the high-resolution structure of the asymmetric protein monomers, provided by MD. The results reveal remarkable differences between the solution and the crystallographic structure of GroEL and between the solution structures of GroEL and of its human homologue Hsp60.

Milrinone is preferred to levosimendan for mesenteric perfusion in hypoxia-reoxygenated newborn piglets treated with dopamine

2012

INTRODUCTION There is little information regarding the comparative hemodynamic effects of adding milrinone or levosimendan to dopamine infusion in hypoxia-reoxygenated (H-R) newborns. RESULTS Severely hypoxic piglets had cardiogenic shock with depressed cardiac index (CI) and mean arterial pressure (MAP). The hemodynamics deteriorated gradually after initial recovery upon reoxygenation. Heart rate and CI improved with milrinone (D+M) and levosimendan (D+L) administration (P < 0.05 vs. control). Both regimens improved carotid arterial flow and carotid vascular resistance; D+M additionally improved superior mesentric arterial flow (all P < 0.05 vs. control). No effect was found on renal arter…

The effect of the type of colloid on the efficacy of hypertonic saline colloid mixtures in hemorrhagic shock: dextran versus hydroxyethyl starch

1993

Colloids increase and prolong the efficacy of hypertonic saline solutions in hemorrhagic shock. We compared the efficacy of dextran 60 and hydroxyethyl starch (HES) 200,000/0.5 at iso-oncotic concentrations of 6.5 or 6% in a 7.5% NaCI solution. Thirty-two rabbits were bled to maintain a mean arterial pressure at 35 mmHg. Twenty-five percent of the shed blood volume was replaced after 40 min by bolus infusion either with hypertonic dextran (HS-DEX) (n = 16) or with hypertonic hydroxyethyl starch (HS-HES) (n = 16). The animals were then observed for a 120-min period. In both groups immediate and complete restoration of cardiovascular function was achieved in up to 30 min and adequate restorat…

Systemic analysis of the caulking assembly process

2017

The present paper highlights the importance of a caulking process which is nowadays less studied in comparison with the growing of its usage in the automotive industry. Due to the fact that the caulking operation is used in domains with high importance such as shock absorbers and brake systems there comes the demand of this paper to detail the parameters which characterize the process, viewed as input data and output data, and the requirements asked for the final product. The paper presents the actual measurement methods used for analysis the performance of the caulking assembly. All this parameters leads to an analysis algorithm of performance established for the caulking process which it …

Management of Direct Oral Anticoagulants in Patients with Atrial Fibrillation Undergoing Cardioversion

2019

Atrial fibrillation the most common cardiac arrhythmia. Its incidence rises steadily with each decade, becoming a real “epidemic phenomenon”. Cardioversion is defined as a rhythm control strategy which, if successful, restores normal sinus rhythm. This, whether obtained with synchronized shock or with drugs, involves a periprocedural risk of stroke and systemic embolism which is reduced by adequate anticoagulant therapy in the weeks before or by the exclusion of left atrial thrombi. Direct oral anticoagulants are safe, manageable, and provide rapid onset of oral anticoagulation; they are an important alternative to heparin/warfarin from all points of view, with a considerable reduction in b…

Variations in physiological responses to thermal stress in congeneric limpets in the Mediterranean Sea

2014

Cardiac activity (Arrhenius breakpoint temperatures and Q10 relationships) and heat shock response (hsp70 expression) were measured in the congeneric limpets Patella rustica, P. caerulea and P. ulyssiponensis in order to test the relationship between their vertical distribution and physiological thermal tolerance. These species exhibit different vertical distributions along Mediterranean shores and despite the narrow tidal range in the Mediterranean, they experience different environmental conditions and consequently had specific thermal windows. Cardiac activity of the upper zoned P. rustica was maintained at higher temperatures than its mid- or low shore counterparts, P. caerulea and P. u…

PspA adopts an ESCRT-III-like fold and remodels bacterial membranes

2020

SummaryPspA is the main effector of the phage shock protein (Psp) system and preserves the bacterial inner membrane integrity and function. Here, we present the 3.6 Å resolution cryo-EM structure of PspA assembled in helical rods. PspA monomers adopt a canonical ESCRT-III fold in an extended open conformation. PspA rods are capable of enclosing lipids and generate positive membrane curvature. Using cryo-EM we visualized how PspA remodels membrane vesicles into μm-sized structures and how it mediates the formation of internalized vesicular structures. Hot spots of these activities are zones derived from PspA assemblies, serving as lipid transfer platforms and linking previously separated lip…

Isolation and characterization of a Paracentrotus lividus cDNA encoding a stress-inducible chaperonin

2001

Chaperonins are ubiquitous proteins that facilitate protein folding in an adenosine triphosphate–dependent manner. Here we report the isolation of a sea urchin cDNA (Plhsp60) coding for mitochondrial chaperonin (Cpn60), whose basal expression is further enhanced by heat shock. The described cDNA corresponds to a full-length mRNA encoding a protein of 582 amino acids, the first 32 of which constitute a putative mitochondrial targeting leader sequence. Comparative analysis has demonstrated that this protein is highly conserved in evolution.