Search results for "Signal Transduction"

showing 10 items of 2020 documents

Ergosterol elicits oxidative burst in tobacco cells via phospholipase A2 and protein kinase C signal pathway

2004

Ergosterol, a typical fungal sterol, induced in tobacco (Nicotiana tabacum L. cv. Xanthi) suspension cells the synthesis of reactive oxygen species and alkalization of the external medium that are dependent on the mobilization of calcium from internal stores. We used specific inhibitors to elucidate the signal pathway triggered by ergosterol compared with cryptogein, a proteinaceous elicitor of Phytophthora cryptogea. HerbimycinA and genistein, inhibitors of tyrosine protein kinases, had no effect on the oxidative burst and pH changes induced by bothelicitors.Similarly,H-89,aninhibitorofproteinkinaseA,hadnoeffectontheinductionofthesedefensereactions.However,theresponse to both elicitors was…

0106 biological sciencesTime FactorsCell SurvivalPhysiologyPlant Science01 natural sciencesPhospholipases AFungal Proteins03 medical and health scienceschemistry.chemical_compoundPhospholipase A2ErgosterolPROTEINE KINASE CTobacco[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biologypolycyclic compoundsGenetics[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyEnzyme InhibitorsEstrenesProtein kinase ACells CulturedProtein Kinase CProtein kinase CComputingMilieux_MISCELLANEOUS030304 developmental biologySulfonamides0303 health sciencesErgosterolbiologyPhospholipase CAlgal ProteinsNeomycinIsoquinolinesPyrrolidinonesSterolElicitorRespiratory burstOxidative StressPhospholipases A2chemistryBiochemistryType C Phospholipasesbiology.proteinlipids (amino acids peptides and proteins)Signal Transduction010606 plant biology & botany
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Cytosolic calcium rises and related events in ergosterol-treated Nicotiana cells

2011

International audience; The typical fungal membrane component ergosterol was previously shown to trigger defence responses and protect plants against pathogens. Most of the elicitors mobilize the second messenger calcium, to trigger plant defences. We checked the involvement of calcium in response to ergosterol using Nicotiana plumbaginifolia and Nicotiana tabacum cv Xanthi cells expressing apoaequorin in the cytosol. First, it was verified if ergosterol was efficient in these cells inducing modifications of proton fluxes and increased expression of defence-related genes. Then, it was shown that ergosterol induced a rapid and transient biphasic increase of free [Ca2þ]cyt which intensity dep…

0106 biological sciencesTime FactorsPhysiologyNicotiana tabacumPlant SciencesterolsSecond Messenger Systemstobacco01 natural scienceschemistry.chemical_compoundCytosolpolycyclic compoundsPhosphorylationCalcium signalingreactive oxygen species0303 health sciencesErgosterolelicitorbiologyergosterolHydrogen-Ion ConcentrationPlants Genetically ModifiedRecombinant ProteinsCell biologyBiochemistrySecond messenger systemReactive oxygen species; Calcium signature; Elicitor; Signal transduction; MAPKs; tobaccolipids (amino acids peptides and proteins)Protonssignal transductionCell Survivalnicotiana plumbaginifoliachemistry.chemical_elementnicotiana tabacumoxydantCalciumcalcium signature03 medical and health sciencesAequorinMAPKsBAPTAGenetics[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyCalcium Signaling030304 developmental biologyMitogen-Activated Protein Kinase KinasesCalcium metabolismHydrogen Peroxidebiochemical phenomena metabolism and nutritionbiology.organism_classificationCytosolchemistryCalciumApoproteins010606 plant biology & botany
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Cross-Talk between ROS and Calcium in Regulation of Nuclear Activities

2010

International audience; Calcium and Reactive Oxygen Species (ROS) are acknowledged as crucial second messengers involved in the response to various biotic and abiotic stresses. However, it is still not clear how these two compounds can play a role in different signaling pathways leading the plant to a variety of processes such as root development or defense against pathogens. Recently, it has been shown that the concept of calcium and ROS signatures, initially discovered in the cytoplasm, can also be extended to the nucleus of plant cells. In addition, it has been clearly proved that both ROS and calcium signals are intimately interconnected. How this cross-talk can finally modulate the tra…

0106 biological sciences[SDV]Life Sciences [q-bio]chemistry.chemical_elementSignallingPlant ScienceBiologyCalciumModels Biological01 natural sciencesNucleus03 medical and health sciencesNuclear proteinCrosstalkMolecular Biology030304 developmental biologyCell NucleusCalcium metabolismchemistry.chemical_classification0303 health sciencesReactive oxygen speciesfungifood and beveragesROSCell biologyMetabolic pathwaychemistryCytoplasmSecond messenger systemCalciumSignal transductionReactive Oxygen SpeciesSignal Transduction010606 plant biology & botanyMolecular Plant
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New frontiers in nitric oxide biology in plant.

2011

Preface; International audience

0106 biological sciences[SDV]Life Sciences [q-bio]nitric oxide synthesisPlant ScienceBiologyNitric Oxide01 natural sciencesperoxynitriteNitric oxide03 medical and health scienceschemistry.chemical_compoundpost-translational protein modificationStress PhysiologicalBotanyGenetics030304 developmental biology0303 health sciencesNitric oxide synthesisHeavy metalsGeneral MedicinePlantsNitric oxide metabolismchemistryPost-translational protein modificationAgronomy and Crop Science010606 plant biology & botanySignal TransductionPlant science : an international journal of experimental plant biology
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The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance agains…

2018

Grapevine (Vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. Chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. The β-glucan laminarin (Lam) and its sulfated derivative (PS3) have been previously demonstrated to induce resistance in grapevine against downy mildew (Plasmopara viticola). However, if Lam elicits classical grapevine defenses such as oxidative burst, pathogenesis-related (PR)-proteins and phytoalexin production, PS3 triggered grapevine resistance via a poorly understood priming phenomenon. The aim of this st…

0106 biological sciencesbeta-Glucanslcsh:MedicineCropsCyclopentanes01 natural sciencesBiochemistryFruitsAgricultural ProductionIntegrated ControlGene Expression Regulation PlantStress Physiological[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyPlant ImmunityVitisOxylipinslcsh:ScienceBiologyGlucansComputingMilieux_MISCELLANEOUSDisease ResistancePlant Diseases2. Zero hungerMultidisciplinaryCell DeathPlant Biochemistrylcsh:R010401 analytical chemistryCell MembraneCrop DiseasesCorrectionAgricultureOrganic FarmingSustainable Agriculture0104 chemical sciences[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyOomyceteslcsh:QPest ControlAgrochemicalsReactive Oxygen SpeciesSalicylic AcidTranscriptome010606 plant biology & botanyResearch ArticleSignal TransductionPLoS ONE
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There's More to the Picture Than Meets the Eye: Nitric Oxide Cross Talk with Ca2+ Signaling

2013

Abstract Calcium and nitric oxide (NO) are two important biological messengers. Increasing evidence indicates that Ca2+ and NO work together in mediating responses to pathogenic microorganisms and microbe-associated molecular patterns. Ca2+ fluxes were recognized to account for NO production, whereas evidence gathered from a number of studies highlights that NO is one of the key messengers mediating Ca2+ signaling. Here, we present a concise description of the current understanding of the molecular mechanisms underlying the cross talk between Ca2+ and NO in plant cells exposed to biotic stress. Particular attention will be given to the involvement of cyclic nucleotide-gated ion channels and…

0106 biological sciencescalmodulinCell signalingCalmodulinPhysiology[SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/AgronomyNanotechnologyPlant ScienceBiology01 natural sciencesNitric oxideTranscriptome03 medical and health scienceschemistry.chemical_compound[ SDV.SA.AGRO ] Life Sciences [q-bio]/Agricultural sciences/Agronomyplant defenseGeneticsPlant defense against herbivoryIon channel030304 developmental biology0303 health sciencescell signallingBiotic stressCell biologychemistryprotein S-nitrosylationgene expressionbiology.proteinplant immunitySignal transduction010606 plant biology & botanyPlant Physiology
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Nitric Oxide Signalling in Plants: Cross-Talk With Ca2+, Protein Kinases and Reactive Oxygen Species

2010

International audience; Nitric oxide (NO) is a gaseous free radical recognized as a ubiquitous signal transducer that contributes to various biological processes in animals. It exerts most of its effects by regulating the activities of various proteins including Ca2+ channels, protein kinases and transcription factors. In plants, studies conducted over the past ten years revealed that NO also functions as an endogenous mediator in diverse physiological processes ranging from root development to stomatal closure. Its biological role as an intracellular plant messenger molecule, however, remains poorly understood. Here, we review the molecular basis of NO signaling in animals and discuss curr…

0106 biological scienceschemistry.chemical_classification[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology0303 health sciencesProgrammed cell deathReactive oxygen speciesKinaseEndogenous mediator01 natural sciencesNitric oxideCell biology03 medical and health scienceschemistry.chemical_compoundchemistry[SDV.BV]Life Sciences [q-bio]/Vegetal BiologySignal transductionTranscription factorIntracellular030304 developmental biology010606 plant biology & botany
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The fungal elicitor cryptogein induces cell wall modifications on tobacco cell suspension

2000

Upon addition of the fungal elicitor cryptogein, suspension cells of tobacco (Nicotiana tabacum cv. Xanthi) aggregated in clusters. Cytochemical experiments indicated that elicited cells displayed fibrillar expansions of pectin along the primary cell wall. Immunocytochemical detection of pectin epitopes indicated that the fibrillar material surrounding the treated cells was mostly composed of low methylated galacturonan sequences, but the use of the cationic probe did not reveal the presence of negatively charged carboxyl groups: the presence of important amounts of calcium ions in these pectic fibrillar expansions accounts for these observations. These data indicate that tobacco cells trea…

0106 biological sciencesfood.ingredientPectinPhysiologyNicotiana tabacumchemistry.chemical_elementPlant ScienceCalcium01 natural sciences[SDV.BV.BOT] Life Sciences [q-bio]/Vegetal Biology/BotanicsCell wallFungal Proteins03 medical and health sciencesfoodCell WallTobaccoCells CulturedComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesbiologyAlgal Proteins[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanicsbiology.organism_classificationImmunohistochemistryElicitorRespiratory burstMicroscopy ElectronPlants ToxicBiochemistrychemistryCell cultureBiophysicsCalciumSignal transduction010606 plant biology & botany
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In vivoanalysis of the lumenal binding protein (BiP) reveals multiple functions of its ATPase domain

2007

International audience; The endoplasmic reticulum (ER) chaperone binding protein (BiP) binds exposed hydrophobic regions of misfolded proteins. Cycles of ATP hydrolysis and nucleotide exchange on the ATPase domain were shown to regulate the function of the ligand-binding domain in vitro. Here we show that ATPase mutants of BiP with defective ATP-hydrolysis (T46G) or ATP-binding (G235D) caused permanent association with a model ligand, but also interfered with the production of secretory, but not cytosolic, proteins in vivo. Furthermore, the negative effect of BiP(T46G) on secretory protein synthesis was rescued by increased levels of wild-type BiP, whereas the G235D mutation was dominant. U…

0106 biological sciencesgenetic structuresRecombinant Fusion ProteinsATPaseBlotting WesternGreen Fluorescent ProteinsPlant ScienceBINDING PROTEINEndoplasmic ReticulumModels Biological01 natural sciencesChromatography Affinity[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health sciencesAdenosine TriphosphateTobaccoPROTEIN FOLDINGGeneticsImmunoprecipitationEndoplasmic Reticulum Chaperone BiPHSP70Heat-Shock Proteins030304 developmental biologyCHAPERONEAdenosine Triphosphatases0303 health sciencesbiologyHydrolysisProtoplastsEndoplasmic reticulumBinding proteinCell BiologyPlants Genetically ModifiedLigand (biochemistry)Secretory proteinBiochemistryChaperone (protein)MutationChaperone bindingbiology.proteinATPASEElectrophoresis Polyacrylamide GelProtein foldingMolecular ChaperonesProtein BindingSignal Transduction010606 plant biology & botanyThe Plant Journal
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Early signaling events induced by elicitors of plant defenses

2006

International audience; Plant pathogen attacks are perceived through pathogenissued compounds or plant-derived molecules that elicit defense reactions. Despite the large variety of elicitors, general schemes for cellular elicitor signaling leading to plant resistance can be drawn. In this article, we review early signaling events that happen after elicitor perception, including reversible protein phosphorylations, changes in the activities of plasma membrane proteins, variations in free calcium concentrations in cytosol and nucleus, and production of nitric oxide and active oxygen species. These events occur within the first minutes to a few hours after elicitor perception. One specific eli…

0106 biological sciencesprotéine kinasePhysiologyNicotiana tabacum[SDV]Life Sciences [q-bio]Biology01 natural sciences03 medical and health sciencesPlant defense against herbivoryProtein kinase A030304 developmental biologyPlant DiseasesPlant Proteinsplant defense responsesprotein kinases0303 health sciencesplant defense responses; ion fluxes; protein kinasesfungifood and beveragesprotein kinaseGeneral Medicineion fluxes;protein kinasePlantsbiology.organism_classificationElicitorCytosolMembrane proteinBiochemistryPhosphorylationionSignal transductionAgronomy and Crop Scienceion fluxes010606 plant biology & botanySignal Transduction
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