Search results for "Skeleton"

Rot1 plays an antagonistic role to Clb2 in actin cytoskeleton dynamics throughout the cell cycle.

2007

ROT1 is an essential gene whose inactivation causes defects in cell cycle progression and morphogenesis in budding yeast. Rot1 affects the actin cytoskeleton during the cell cycle at two levels. First, it is required for the maintenance of apical growth during bud growth. Second, Rot1 is necessary to polarize actin cytoskeleton to the neck region at the end of mitosis; because of this defect, rot1 cells do not properly form a septum to complete cell division. The inability to polarize the actin cytoskeleton at the end of mitosis is not due to a defect in the recruitment of the polarisome scaffold protein Spa2 or the actin cytoskeleton regulators Cdc42 and Cdc24 in the neck region. Previous …

Proprotein convertase 5/6 is critical for embryo implantation in women: regulating receptivity by cleaving ebp50, modulating ezrin binding, and membr…

2011

Establishment of endometrial receptivity is vital for successful embryo implantation; its failure causes infertility. Epithelial receptivity acquisition involves dramatic structural changes in the plasma membrane and cytoskeleton. Proprotein convertase 5/6 (PC6), a serine protease of the proprotein convertase (PC) family, is up-regulated in the human endometrium specifically at the time of epithelial receptivity and stromal cell decidualization. PC6 is the only PC member tightly regulated in this manner. The current study addressed the importance and mechanisms of PC6 action in regulating receptivity in women. PC6 was dysregulated in the endometrial epithelium during the window of implantat…

Macroporous Scaffolds Based on Chitosan and Bioactive Molecules†

2007

Chitosan-based macroporous scaffolds for tissue engineering applications are developed by cryogelation in aqueous media. The cryogels obtained are modified using a new RGD-containing peptide developed in this laboratory. A RGD-containing peptide is chemically attached to the surface of the cryogels to improve cell adhesion to the 3D-structure chitosan-based scaffolds. The synthesis, physico-chemical, and biological evaluations of the system are described, and the optimization of the formulations is carried out by varying the reaction parameters. Fibroblasts and endothelial cells are used in cell cultures to determine cell behavior and the cytocompatibility of the macroporous cryogels. Cell …

Inhibition of FcεRI-mediated Activation of Rat Basophilic Leukemia Cells by Clostridium difficile Toxin B (Monoglucosyltransferase)

1996

Abstract Treatment of rat basophilic leukemia (RBL) 2H3-hm1 cells with Clostridium difficile toxin B (2 ng/ml), which reportedly depolymerizes the actin cytoskeleton, blocked [3H]serotonin release induced by 2,4-dinitrophenyl-bovine serum albumin, carbachol, mastoparan, and reduced ionophore A23187-stimulated degranulation by about 55-60%. In lysates of RBL cells, toxin B 14C-glucosylated two major and one minor protein. By using two-dimensional gel electrophoresis and immunoblotting, RhoA and Cdc42 were identified as protein substrates of toxin B. In contrast to toxin B, Clostridium botulinum transferase C3 that selectively inactivates RhoA by ADP-ribosylation did not inhibit degranulation…

Sensitivity of Regional Water Supply Systems Models to the Level of Skeletonization – A Case Study from Apulia, Italy

2015

AbstractSimulation models supported by state-of-the-art software packages are nowadays available to explore operation rules of regional water supply systems or to select structural alternatives for improving long-term service performances.Given the, sometimes high, complexity of these systems, model calibration can become a lengthy procedure and many runs are necessary before obtaining convincing results. However, even after calibration, depending on system's complexity and the number of time steps investigated, a single run can take up to several minutes, even on state-of-the-art computers, so that simulation time can become a true bottleneck if such models are to be coupled with metaheuri…

Are control of extracellular acid-base balance and regulation of skeleton genes linked to resistance to ocean acidification in adult sea urchins?

2020

SCOPUS: ar.j

Graph-based minimal path tracking in the skeleton of the retinal vascular network

2012

This paper presents a semi-automatic framework for minimal path tracking in the skeleton of the retinal vascular network. The method is based on the graph structure of the vessel network. The vascular network is represented based on the skeleton of the available segmented vessels and using an undirected graph. Significant points on the skeleton are considered nodes of the graph, while the edge of the graph is represented by the vessel segment linking two neighboring nodes. The graph is represented then in the form of a connectivity matrix, using a novel method for defining vertex connectivity. Dijkstra and Floyd-Warshall algorithms are applied for detection of minimal paths within the graph…

3D skeleton-based human action classification: A survey

2016

In recent years, there has been a proliferation of works on human action classification from depth sequences. These works generally present methods and/or feature representations for the classification of actions from sequences of 3D locations of human body joints and/or other sources of data, such as depth maps and RGB videos.This survey highlights motivations and challenges of this very recent research area by presenting technologies and approaches for 3D skeleton-based action classification. The work focuses on aspects such as data pre-processing, publicly available benchmarks and commonly used accuracy measurements. Furthermore, this survey introduces a categorization of the most recent…

Colmare il gap nelle competenze di bio-ricostruzione di scheletri di Cetacei in Sicilia

The Unique Invention of the Siliceous Sponges: Their Enzymatically Made Bio-Silica Skeleton

2011

Sponges are sessile filter feeders that, among the metazoans, evolved first on Earth. In the two classes of the siliceous sponges (the Demospongiae and the Hexactinellida), the complex filigreed body is stabilized by an inorganic skeleton composed of amorphous silica providing them a distinct body shape and plan. It is proposed that the key innovation that allowed the earliest metazoans to form larger specimens was the enzyme silicatein. This enzyme is crucial for the formation of the siliceous skeleton. The first sponge fossils with body preservation were dated back prior to the “Precambrian-Cambrian” boundary [Vendian (610–545 Ma)/Ediacaran (542–580 Ma)]. A further molecule required for t…