6533b871fe1ef96bd12d24f5
RESEARCH PRODUCT
Macroporous Scaffolds Based on Chitosan and Bioactive Molecules†
María Rosa AguilarMaría Auxiliadora PrietoB. BondarH. KirsebomJ. San RománMar Fernándezsubject
ScaffoldPolymers and Plastics0206 medical engineeringCellBioengineeringPeptideNanotechnology02 engineering and technologyActin cytoskeleton organizationlaw.inventionScaffoldBiomaterialsChitosanchemistry.chemical_compoundTissue engineeringConfocal microscopylawMaterials ChemistrymedicineCell adhesionchemistry.chemical_classificationChitosanRGDChemistryCytocompatibility021001 nanoscience & nanotechnology020601 biomedical engineeringmedicine.anatomical_structureChemical engineering0210 nano-technologyCryogelsdescription
Chitosan-based macroporous scaffolds for tissue engineering applications are developed by cryogelation in aqueous media. The cryogels obtained are modified using a new RGD-containing peptide developed in this laboratory. A RGD-containing peptide is chemically attached to the surface of the cryogels to improve cell adhesion to the 3D-structure chitosan-based scaffolds. The synthesis, physico-chemical, and biological evaluations of the system are described, and the optimization of the formulations is carried out by varying the reaction parameters. Fibroblasts and endothelial cells are used in cell cultures to determine cell behavior and the cytocompatibility of the macroporous cryogels. Cell spreading and actin cytoskeleton organization process are assessed by confocal microscopy. Cells colonize the porous structure of the chitosan-based cryogel and are observed to be growing inside the pores.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-11-01 | Journal of Bioactive and Compatible Polymers |