0000000000073318
AUTHOR
María Auxiliadora Prieto
Development of a Higee bioreactor (HBR) for production of polyhydroxyalkanoate: Hydrodynamics, gas–liquid mass transfer and fermentation studies
Abstract This study addresses the hydrodynamics and mass transfer characterisation of a Higee bioreactor (HBR) for application to polyhydroxyalkanoate (PHA) production from Pseudomonas putida KT2442 fermentation. The motivation for this work is to address the potential oxygen transfer limitations which can severely impede the progress of this aerobic fermentation process and reduce PHA productivity in conventional bioreactors. It is shown that a maximum of 2.5 transfer units can be achieved in an oxygen-stripping operation where the presence of packing, higher rotor speeds, higher air flowrates and lower liquid flowrates all have a positive influence on the number of transfer units (NTU). W…
In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain
15 p.-6 fig.-6 tab.
Genome Sequence of the Methanotrophic Poly-β-Hydroxybutyrate Producer Methylocystis parvus OBBP
-- PAGS 2 5709-5710
Macroporous Scaffolds Based on Chitosan and Bioactive Molecules†
Chitosan-based macroporous scaffolds for tissue engineering applications are developed by cryogelation in aqueous media. The cryogels obtained are modified using a new RGD-containing peptide developed in this laboratory. A RGD-containing peptide is chemically attached to the surface of the cryogels to improve cell adhesion to the 3D-structure chitosan-based scaffolds. The synthesis, physico-chemical, and biological evaluations of the system are described, and the optimization of the formulations is carried out by varying the reaction parameters. Fibroblasts and endothelial cells are used in cell cultures to determine cell behavior and the cytocompatibility of the macroporous cryogels. Cell …