Search results for "Small"

showing 10 items of 2441 documents

Stat3 and Gfi-1 Transcription Factors Control Th17 Cell Immunosuppressive Activity via the Regulation of Ectonucleotidase Expression

2012

International audience; Although Th17 cells are known to promote tissue inflammation and autoimmunity, their role during cancer progression remains elusive. Here, we showed that in vitro Th17 cells generated with the cytokines IL-6 and TGF-β expressed CD39 and CD73 ectonucleotidases, leading to adenosine release and the subsequent suppression of CD4(+) and CD8(+) T cell effector functions. The IL-6-mediated activation of the transcription factor Stat3 and the TGF-β-driven downregulation of Gfi-1 transcription factor were both essential for the expression of ectonucleotidases during Th17 cell differentiation. Stat3 supported whereas Gfi-1 repressed CD39 and CD73 expression by binding to thei…

Adoptive cell transferMESH : Transcription FactorsCellular differentiationMESH: Th17 CellsT-LymphocytesCellMESH : Promoter Regions GeneticMESH : RNA Small InterferingMESH: Mice KnockoutMice0302 clinical medicineTransforming Growth Factor betaMESH: RNA Small InterferingMESH : STAT3 Transcription FactorImmunology and Allergy[ SDV.IMM ] Life Sciences [q-bio]/ImmunologyEctonucleotidaseMESH: AnimalsRNA Small InterferingSTAT3MESH: Lymphocytes Tumor-InfiltratingPromoter Regions GeneticMESH: Antigens CD5'-NucleotidaseRegulation of gene expressionMice Knockout0303 health sciencesMESH : Gene Expression RegulationApyraseMESH: STAT3 Transcription FactorMESH: Transcription FactorsMESH: Gene Expression RegulationMESH : Mice TransgenicCell biologyMESH : Lymphocytes Tumor-InfiltratingDNA-Binding ProteinsMESH : ApyraseInfectious Diseasesmedicine.anatomical_structure[SDV.IMM]Life Sciences [q-bio]/ImmunologyMESH : DNA-Binding ProteinsMESH: ApyraseSTAT3 Transcription Factor[SDV.IMM] Life Sciences [q-bio]/ImmunologyMESH : Interleukin-6MESH: Mice TransgenicT cellImmunologyMice TransgenicMESH : Mice Inbred C57BLBiology03 medical and health sciencesLymphocytes Tumor-InfiltratingMESH: Mice Inbred C57BLAntigens CDMESH: Promoter Regions GeneticMESH : 5'-NucleotidaseMESH : MicemedicineMESH : Antigens CDMESH : Th17 CellsAnimalsTranscription factorMESH: MiceMESH: Transforming Growth Factor beta030304 developmental biologyMESH : T-LymphocytesBinding SitesInterleukin-6MESH: Interleukin-6Mice Inbred C57BLMESH: T-LymphocytesMESH : Transforming Growth Factor betaMESH: Binding SitesGene Expression Regulationbiology.proteinMESH : Mice KnockoutTh17 CellsMESH : AnimalsMESH: 5'-NucleotidaseMESH: DNA-Binding ProteinsMESH : Binding Sites030215 immunologyTranscription FactorsImmunity
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A Retrospective Cross-Sectional Population-Based Study on Prenatal Levels of Adherence to the Mediterranean Diet: Maternal Profile and Effects on the…

2018

The Mediterranean diet (MD) is a dietary pattern with important benefits. The objectives of this study were to assess the adherence to the MD among pregnant women in Valencia (Spain) and characterize the pregnant women according to their level of adherence. Finally, we aimed to examine the role of MD adherence during pregnancy in the anthropometric development of the newborn. The study included 492 pregnant women who were followed at La Fe Hospital in 2017. The self-administered &ldquo

Adult0301 basic medicinemedicine.medical_specialtyAdolescentMediterranean dietHealth Toxicology and Mutagenesisprimary preventionlcsh:MedicineDiet MediterraneanArticleFetal DevelopmentYoung Adult03 medical and health sciencessmall for gestational ageMediterranean dietOdds RatioHumansMedicinePrenatal Nutritional Physiological PhenomenaRetrospective StudiesPregnancy030109 nutrition & dieteticsbusiness.industryObstetricsConfoundinglcsh:RInfant NewbornPublic Health Environmental and Occupational HealthOdds ratioAnthropometrymedicine.diseaseConfidence intervalCross-Sectional StudiesSpainPopulation SurveillanceInfant Small for Gestational AgeSmall for gestational ageFemaleGuideline AdherencePregnant WomenpregnancyDiet HealthybusinessBody mass index
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Corrigendum: Natural History of Non-Small-Cell Lung Cancer with Bone Metastases.

2016

We conducted a large, multicenter, retrospective survey aimed to explore the impact of tumor bone involvement in Non-Small Cell Lung Cancer.Data on clinical-pathology, skeletal outcomes and bone-directed therapies for 661 deceased patients with evidence of bone metastasis were collected and statistically analyzed. Bone metastases were evident at diagnosis in 57.5% of patients. In the remaining cases median time to bone metastases appearance was 9 months. Biphosphonates were administered in 59.6% of patients. Skeletal-related events were experienced by 57.7% of patients; the most common was the need for radiotherapy. Median time to first skeletal-related event was 6 months. Median survival a…

AdultAged 80 and overMaleLung NeoplasmsBone NeoplasmsMiddle AgedCorrigendaArticleYoung AdultCarcinoma Non-Small-Cell LungDisease ProgressionHumansFemaleAgedScientific reports
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Effect of lactose, lactulose and bisacodyl on gastrointestinal transit studied by metal detector.

2007

SUMMARY Aim and methods: To study the effect of 45 g lactose, 30 g lactulose and 10 mg bisacodyl on gastrointestinal transit in 30 healthy volunteers by metal detector and Hinton marker method. The first set of measurements were performed under standard conditions. In a second stage, transit was slowed to twice the original value by loperamide to simulate constipation conditions. Results: Bisacodyl drastically accelerated small and large intestinal transit. Colonic transit was shortened to 23 % and to 31% of control values, without and with loperamide. Bisacodyl increased stool weight and decreased stool consistency in all persons. Lactulose marginally shortened small intestinal transit (P=…

AdultBisacodylMalemedicine.medical_specialtyLoperamideConstipationColonmedicine.medical_treatmentLaxativeLactoseGastroenterologyLoperamideLactulosechemistry.chemical_compoundInternal medicineIntestine SmallmedicineHumansPharmacology (medical)Intestine LargeBisacodylLactoseDefecationGastrointestinal TransitHepatologyGastrointestinal transitdigestive oral and skin physiologyGastroenterologyLarge intestinalLactuloseMicrosphereschemistryGastric EmptyingFemalemedicine.symptommedicine.drugAlimentary pharmacologytherapeutics
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Enhancement pattern of small hepatocellular carcinoma (HCC) at contrast-enhanced US (CEUS), MDCT, and MRI: intermodality agreement and comparison of …

2012

OBJECTIVE:To evaluate agreement between contrast-enhanced ultrasound (CEUS), multi-detector row computed tomography (MDCT) and magnetic resonance imaging (MRI) for the assessment of typical and atypical enhancement patterns of small hepatocellular carcinoma (HCC); and to compare diagnostic sensitivity of 2005 and 2010 American Association for the Study of Liver Diseases (AASLD) guidelines. MATERIALS AND METHODS: Between January 2008 and December 2009, we included cirrhotic patients with newly diagnosed 10-20 mm HCC imaged at two contrast-enhanced imaging techniques among CEUS, MDCT, and MRI. Dynamic studies were reviewed by two radiologists to assess enhancement pattern. Percentage of cases…

AdultDiagnostic ImagingMalemedicine.medical_specialtyCarcinoma HepatocellularContrast MediaEnhancement patternSensitivity and SpecificityImaging modalitiesTumor enhancementMcNemar's testmedicineHumansRadiology Nuclear Medicine and imagingAgedAged 80 and overmedicine.diagnostic_testbusiness.industryLiver NeoplasmsUltrasoundReproducibility of ResultsMagnetic resonance imagingGeneral MedicineMiddle Agedmedicine.diseaseUnited Statessmall hepatocellular carcinomaItalyHepatocellular carcinomaPractice Guidelines as TopicFemaleRadiologybusinesscontrast-enhanced ultrasoundContrast-enhanced ultrasound
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Review with novel markers facilitates precise categorization of 41 cases of diagnostically challenging, “undifferentiated small round cell tumors”. A…

2017

Abstract Background Despite extensive immunohistochemical (IHC) and molecular studies combined with morphologic findings, a group of round/ovoid cell tumors histologically similar to Ewing sarcomas (ES) but lacking EWSR1-rearrangements may remain unclassifiable. Design We retrospectively analyzed 41 Ewing-like tumors (formalin-fixed, paraffin-embedded) previously determined as negative or non-informative for EWSR1-rearrangements by FISH and/or RT-PCR. A new histopathology revision and additional IHC and molecular analyses were carried out in order to investigate whether additional IHC and/or molecular testing in combination with the morphological findings may help in reaching a definitive d…

AdultMale0301 basic medicinePathologymedicine.medical_specialtyAdolescentDesmoplastic small-round-cell tumorSarcoma EwingSclerosing rhabdomyosarcomaImmunophenotypingPathology and Forensic MedicineYoung Adult03 medical and health sciences0302 clinical medicineBiomarkers TumormedicineHumansStromal tumorChildAgedRetrospective StudiesHomeodomain ProteinsGiSTbusiness.industryNuclear ProteinsCell DifferentiationGeneral MedicineMiddle AgedPrognosismedicine.diseaseImmunohistochemistrySynovial sarcomaMolecular TypingHomeobox Protein Nkx-2.2030104 developmental biology030220 oncology & carcinogenesisSarcoma Small CellFemaleSarcomaClear-cell sarcomaRNA-Binding Protein EWSbusinessTranscription FactorsMyoepithelial TumorAnnals of Diagnostic Pathology
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Isolation in small populations of Wayampi Amerindians promotes endemicity and homogenisation of their faecal virome, but its distribution is not enti…

2018

The isolated community of the Wayampi Amerindians has been extensively studied for the presence of beta lactamase-producing enterobacteria and their gut microbiota. However, no information about their virome was available. This study tries to establish potential associations between the virome and diverse epidemiological data, through the metagenomic study of the faecal prophages and DNA viruses from 31 samples collected in 2010. Taxonomic assignments, composition, abundance and diversity analyses were obtained to characterise the virome and were compared between groups according to several demographic, environmental and medical data. Prophages outnumbered viruses. Composition and abundance…

AdultMale0301 basic medicineProphagesBeta diversityZoologyGut floraGroup comparisonApplied Microbiology and BiotechnologyMicrobiologybeta-LactamasesFeces03 medical and health sciencesEnterobacteriaceaeHumansHuman viromeFecesEcologybiologyViromeSingle factorDNA VirusesHigh-Throughput Nucleotide SequencingSmall population sizebiology.organism_classificationAnti-Bacterial AgentsFrench GuianaGastrointestinal MicrobiomeIsolated human population030104 developmental biologyLow exposure to antibioticsMetagenomicsNext-generation sequencingFemaleMetagenomicsFEMS Microbiology Ecology
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A First-in-Human Phase I Study of the ATP-Competitive AKT Inhibitor Ipatasertib Demonstrates Robust and Safe Targeting of AKT in Patients with Solid …

2016

Abstract Activation of AKT signaling by PTEN loss or PIK3CA mutations occurs frequently in human cancers, but targeting AKT has been difficult due to the mechanism-based toxicities of inhibitors that target the inactive conformation of AKT. Ipatasertib (GDC-0068) is a novel selective ATP-competitive small-molecule inhibitor of AKT that preferentially targets active phosphorylated AKT (pAKT) and is potent in cell lines with evidence of AKT activation. In this phase I study, ipatasertib was well tolerated; most adverse events were gastrointestinal and grade 1–2 in severity. The exposures of ipatasertib ≥200 mg daily in patients correlated with preclinical TGI90, and pharmacodynamic studies co…

AdultMale0301 basic medicineProto-Oncogene Proteins c-aktAdministration OralPharmacologyIpatasertibDrug Administration SchedulePiperazines03 medical and health sciences0302 clinical medicineCell Line TumorNeoplasmsHumansPTENMedicineProtein Kinase InhibitorsProtein kinase BPI3K/AKT/mTOR pathwayAgedbiologybusiness.industryMiddle AgedXenograft Model Antitumor AssaysSmall moleculePyrimidines030104 developmental biologyOncologyCell culture030220 oncology & carcinogenesisPharmacodynamicsbiology.proteinFemalebusinessProto-Oncogene Proteins c-akt
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Circulating miR-323-3p is a biomarker for cardiomyopathy and an indicator of phenotypic variability in Friedreich’s ataxia patients

2017

AbstractMicroRNAs (miRNAs) are noncoding RNAs that contribute to gene expression modulation by regulating important cellular pathways. In this study, we used small RNA sequencing to identify a series of circulating miRNAs in blood samples taken from Friedreich’s ataxia patients. We were thus able to develop a miRNA biomarker signature to differentiate Friedreich’s ataxia (FRDA) patients from healthy people. Most research on FDRA has focused on understanding the role of frataxin in the mitochondria, and a whole molecular view of pathological pathways underlying FRDA therefore remains to be elucidated. We found seven differentially expressed miRNAs, and we propose that these miRNAs represent …

AdultMale0301 basic medicineSmall RNAAtaxiaSciencePopulationCardiomyopathyBioinformaticsArticleYoung Adult03 medical and health sciencesmicroRNAmedicineHumanseducationCells CulturedAgedCell ProliferationGeneticseducation.field_of_studyMultidisciplinarybiologyQRHigh-Throughput Nucleotide SequencingMiddle AgedPrognosismedicine.diseasePhenotypeMicroRNAs030104 developmental biologyBiological Variation PopulationFriedreich AtaxiaCase-Control StudiesFrataxinbiology.proteinBiomarker (medicine)MedicineFemalemedicine.symptomCardiomyopathiesBiomarkersFollow-Up StudiesScientific Reports
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MUC4 impairs the anti-inflammatory effects of corticosteroids in patients with chronic rhinosinusitis with nasal polyps

2017

Background Current evidence suggests that membrane-tethered mucins could mediate corticosteroid efficacy, interacting with glucocorticoid receptor (GR) in patients with chronic rhinosinusitis with nasal polyps (CRSwNP). Mucin 4 (MUC4)–tethered mucin is expressed in nasal polyp (NP) epithelial cells and upregulated under inflammatory conditions. Moreover, MUC4β has the capacity to interact with other intracellular proteins. We hypothesized that MUC4 modulates corticosteroid efficacy of patients with CRSwNP. Objective We sought to analyze the role of MUC4 in corticosteroid effectiveness in different cohorts of patients with CRSwNP and elucidate the possible mechanisms involved. Methods Eighty…

AdultMale0301 basic medicineSmall interfering RNAmedicine.drug_classImmunologyAnti-Inflammatory AgentsDrug ResistanceBiologyDexamethasoneCell LineYoung Adult03 medical and health sciencesNasal PolypsGlucocorticoid receptorPregnenedionesmedicineglucocorticoid receptorHumansImmunology and AllergyNasal polypsSinusitisCells CulturedDexamethasoneAgedRhinitisnasal polypGene knockdownMucin-4chronic rhinosinusitisMucincorticosteroid resistanceEpithelial CellsMiddle Agedmedicine.diseaseHEK293 Cells030104 developmental biologyMUC4Chronic DiseaseImmunologyCorticosteroidImmunohistochemistryFemalesense organsmedicine.drug
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