Search results for "Solutions"

A paradigm of extreme rainfall pluvial floods in complex urban areas: the flood event of 15 July 2020 in Palermo (Italy)

2021

Abstract. In the last few years, some regions of the Mediterranean area have witnessed a progressive increase in extreme events, such as urban and flash floods, as a response to the increasingly frequent and severe extreme rainfall events, which are often exacerbated by the ever-growing urbanization. In such a context, the urban drainage systems may not be sufficient to convey the rainwater, thus increasing the risk deriving from the occurrence of such events. This study focuses on a particularly intense urban flood that occurred in Palermo (Italy) on 15 July 2020; it represents a typical pluvial flood due to extreme rainfall on a complex urban area that many cities have experienced in rece…

Dimer-tetramer transition between solution and crystalline states of streptavidin and avidin mutants.

2003

ABSTRACT The biotin-binding tetrameric proteins, streptavidin from Streptomyces avidinii and chicken egg white avidin, are excellent models for the study of subunit-subunit interactions of a multimeric protein. Efforts are thus being made to prepare mutated forms of streptavidin and avidin, which would form monomers or dimers, in order to examine their effect on quaternary structure and assembly. In the present communication, we compared the crystal structures of binding site W→K mutations in streptavidin and avidin. In solution, both mutant proteins are known to form dimers, but upon crystallization, both formed tetramers with the same parameters as the native proteins. All of the intersub…

Stress fields in general composite laminates

1996

A direct approach is employed to obtain a general boundary integral formulation for the analysis of composite laminates subjected to uniform axial strain. The integral equations governing the problem are directly deduced from the reciprocity theorem, employing the generalized orthotropic elasticity fundamental solutions expressly inferred. The solution is achieved by the boundary element method, which gives, once the traction-free boundary conditions and the interfacial continuity conditions are enforced, a linear system of algebraic equations. The formulation does not present restrictions with regard to the laminate stacking sequence and it does not require any aprioristic assumption. The …

Nonlinear Nonhomogeneous Elliptic Problems

2019

We consider nonlinear elliptic equations driven by a nonhomogeneous differential operator plus an indefinite potential. The boundary condition is either Dirichlet or Robin (including as a special case the Neumann problem). First we present the corresponding regularity theory (up to the boundary). Then we develop the nonlinear maximum principle and present some important nonlinear strong comparison principles. Subsequently we see how these results together with variational methods, truncation and perturbation techniques, and Morse theory (critical groups) can be used to analyze different classes of elliptic equations. Special attention is given to (p, 2)-equations (these are equations driven…

THE MINIMIZING TOTAL VARIATION FLOW WITH MEASURE INITIAL CONDITIONS

2004

In this paper we obtain existence and uniqueness of solutions for the Cauchy problem for the minimizing total variation flow when the initial condition is a Radon measure in ℝN. We study limit solutions obtained by weakly approximating the initial measure μ by functions in L1(ℝN). We are able to characterize limit solutions when the initial condition μ=h+μs, where h∈L1(ℝN)∩L∞(ℝN), and μs=αℋk⌊ S,α≥0,k is an integer and S is a k-dimensional manifold with bounded curvatures. In case k<N-1 we prove that the singular part of the solution does not move, it remains equal to μs for all t≥0. In particular, u(t)=δ0 when u(0)=δ0. In case k=N-1 we prove that the singular part of the limit solution …

Ascorbic Acid Oxidation in Sucrose Aqueous Model Systems at Subzero Temperatures

2004

The reduction of Tempol by ascorbic acid in concentrated sucrose solutions was measured by electron paramagnetic resonance (EPR) at temperatures ranging from 16 to −16 °C. This method allowed the determination of the rate constants (k) of this fast reaction, by recording the Tempol reduction as a function of time. The two reactants were initially separated and had to migrate for the reaction to occur. The experimental findings were compared with predicted values according to the equation for diffusion-controlled reaction proposed by Atkins. The experimental reaction rate constants were observed to be lower than the calculated ones. However, the experimental values were found to be controlle…

Thermal Aggregation of Bovine Serum Albumin in Trehalose and Sucrose Aqueous Solutions

2012

We report results of static and dynamic light scattering measurements performed on bovine serum albumin (BSA) in saccharide (trehalose and sucrose) solutions. Our aim is to study the effects of the two disaccharides on the first steps of thermal aggregation of BSA in aqueous solutions at two protein concentrations (1 and 30 mg/mL) at increasing sugar/water ratio. Results show that sugars modify early stages of aggregation mainly by perturbing the thermodynamic behavior of the solvent (i.e., general solvent effects) without involving direct, specific sugar-protein interactions. This agrees with current hypotheses on sugar action in protein solutions. (1-3) The linear correlation detected bet…

Impact of Destroying the Structure of Model Gels on Volatile Release

2007

International audience; The release of a strawberry aroma from different composite gels taken as models of fruit preparations and from a sucrose solution was investigated. The composition of the model systems differed with regard to the gelling agent, either pectin or carrageenan, and to the rigidity of the gel. With the use of atmospheric pressure chemical ionization−mass spectrometry, the release profiles of the aroma compounds were determined under stirring. At the same time, purge and trap measurements were performed to determine the release profiles of the aroma compounds without stirring. The comparison of the patterns obtained using these two complementary methods made it possible to…

Molecular surface area and hydrophobic effect.

1992

Multi-residue analysis of 30 currently used pesticides in fine airborne particulate matter (PM 2.5) by microwave-assisted extraction and liquid chrom…

2009

A confirmatory and rapid procedure has been developed for the determination of 30 currently used pesticides (CUP) in fine airborne particulate matter (PM 2.5) at trace level. The proposed method includes extraction of PM 2.5-bound pesticides by microwave-assisted extraction (MAE) followed by a direct injection into LC-MS/MS. The main parameters affecting the MAE extraction (time, temperature and volume of solvent) were optimised using statistical design of experiments (DoE). The matrix effect was also evaluated. Recoveries ranged from 72 to 109% and the limit of quantification (LoQ) was 32.5 pg m(-3) for chlorpyrifos, 13.5 pg m(-3) for fenhexamid, imazalil and prochloraz, and 6.5 pg m(-3) f…