Search results for "Sorghum"

Presence of mycotoxins in Tunisian infant foods samples and subsequent risk assessment

2018

Abstract A study on a set of cereal and cereal-based products (n = 117) intended for infant consumption was performed to determine the natural presence of twenty different mycotoxins by both liquid chromatography and gas chromatography coupled to tandem mass spectrometry (MS/MS). Analytical data showed that 67% of analyzed samples were contaminated by at least one mycotoxin at variable levels. Deoxynivalenol presented the highest incidence (38%) and reached a maximum level of 240 ng/g in a bsissa sample (oat product), higher than the maximum level set for DON in cereal products (200 ng/g). While, enniatin B was found in 25 samples with a maximum level of 316 ng/g found also in a bsissa samp…

Determination of Fusarium mycotoxins enniatins, beauvericin and fusaproliferin in cereals and derived products from Tunisia

2011

Abstract In this study, 51 samples of cereals (wheat, Barley, maize and Sorghum) and by-products (mainly pasta and couscous) purchased from Tunisian supermarkets were examined for contamination with the emerging Fusarium mycotoxins: Enniatins ENs (EN A, EN A 1 , EN B and EN B 1 ), beauvericin (BEA) and fusaproliferin (FUS). The extraction of the samples was performed with methanol using an Ultra-turrax homogenizer. Mycotoxins were analyzed with a liquid chromatography (LC) coupled to a diode array detector (DAD). The frequencies of contamination of total samples with ENs were 96%. EN A 1 was the most common EN found with the highest prevalence of 92.1%, levels ranged between 11.1 and 480 mg…

Isolation and partial characterization of antagonistic peptides produced by Paenibacillus sp. strain B2 isolated from the sorghum mycorrhizosphere.

2005

ABSTRACT Paenibacillus sp. strain B2, isolated from the mycorrhizosphere of sorghum colonized by Glomus mosseae , produces an antagonistic factor. This factor has a broad spectrum of activity against gram-positive and gram-negative bacteria and also against fungi. The antagonistic factor was isolated from the bacterial culture medium and purified by cation-exchange, reverse-phase, and size exclusion chromatography. The purified factor could be separated into three active compounds following characterization by amino acid analysis and by combined reverse-phase chromatography and mass spectrometry (liquid chromatography-mass spectrometry and mass spectrometry-mass spectrometry). The first com…

Presence of mycotoxins in sorghum and intake estimation in Tunisia

2014

Sorghum samples (n = 60) from Tunisian markets were analysed for the occurrence of 22 of both traditional and emerging mycotoxins. Samples were extracted with a QuEChERS-like method and mycotoxins were detected by LC-MS/MS. This method was validated and adequate analytical parameters were obtained. All samples had contamination with mycotoxins and several samples had higher contamination levels than European Union legislative limits (MLs). The most frequently found mycotoxins were ENB (100%), OTA (98%), ENA₁ (63%), ENB₁ (56%), BEA (48%), AFB1 (38%) and STG (33%). Mean contaminations were 30.7, 1.93, 33.2, 51.0, 15.4, 1.49 and 20.5 µg kg(-1), respectively. While two samples were contaminated…

Cropping system dynamics, climate variability, and seed losses among East African smallholder farmers: a retrospective survey.

2014

Abstract Climate variability directly affects traditional low input and rain-fed farming systems, but few studies have paid attention retrospectively to the cropping system’s ability to mitigate climate risk. This study analyzes the impacts of rainfall variability on farmers’ seed variety losses over time, considering changes in smallholder farming systems. The cropping system dynamics, in favoring maize at the expense of sorghum and pearl millet, have induced an increasing risk of seed loss during drought. Combining ecological anthropology and climatology, a retrospective survey asking farmers about the period 1961–2006 was carried out at three altitudinal levels (750, 950, and 1100 m) on …

Expression of Sorghum bicolor ammonium transporters upon colonization with arbuscular mycorrhizal fungi

2012

Arbuscular mycorrhizal fungi (AMF) are important plant symbionts, trading mineral nutrients beyond the reach of roots, in particular ammonium, in exchange to their host’s photosynthetic products. Sorghum bicolor is one of the world's leading cereal crops, providing food, fibre and fuel across a range of environments and production systems. It has a particular ability to be productive even under strongly adverse conditions, tolerating much more severe drought than most other grain crops. As its genome has recently been sequenced, we have characterized all eight members of the ammonium transporter (AMT) family and studied their expression in different tissues of field-grown plants. While most…

Effects of Inter-cropping and a Herbicide on Management of Striga hermonthica on Sorghum

2017

Aims: The present work was undertaken to determine the effects of the herbicide chlorsulfuron and intercropping sorghum (cv.Wad-Ahmed) with cowpea (T100K-901-6 cv.) on S. hermonthica incidence and sorghum growth. Study Design: Randomized Complete Block Design (RCBD) with four replicates. Place and Duration of Study: A series of laboratory and greenhouse experiments was undertaken at the College of Agricultural Studies, Sudan University of Science and Technology (SUST) at Shambat, during the season 2013/2014. Methodology: Striga free or infested soil was placed in plastic pots (9 cm i .d) with perforations at the bottoms. Pots filled with Striga free soil (0 mg) were included as control for …

Hard Cap Espresso Machine Extraction of Polyphenolic Compounds from Pulses

2018

A hard cap espresso machine was employed for the extraction of polyphenolic compounds from food samples as dry legumes and pulses. 100 mg of ground dry sample was mixed with a dispersing agent and placed in a refillable stainless steel capsule with a borosilicate filter. A complete polyphenol extraction was achieved by using 150 mL ethanol 50 % (v/v) in water and determined by Folin-Ciocalteu spectrophotometric method. The method provided a limit of detection of 0.4 mg g<sup>-1</sup>. The whole extraction procedure was achieved in less than one minute using the mild extraction conditions of 72°C and 19 bars given by the domestic machine. Fifteen varieties of food samples (3 lent…

Metal distribution in the process of lignocellulosic ethanol production from heavy metal contaminated sorghum biomass

2016

Determining water use of sorghum from two-source energy balance and radiometric temperatures

2011

Estimates of surface actual evapotranspiration (ET) can assist in predicting crop water requirements. An alternative to the traditional crop-coefficient methods are the energy balance models. The objective of this research was to show how surface temperature observations can be used, together with a two-source energy balance model, to determine crop water use throughout the different phenological stages of a crop grown. Radiometric temperatures were collected in a sorghum (<i>Sorghum bicolor</i>) field as part of an experimental campaign carried out in Barrax, Spain, during the 2010 summer growing season. Performance of the Simplified Two-Source Energy Balance (STSEB) model was …