Search results for "Sorghum"

Metal distribution in the process of lignocellulosic ethanol production from heavy metal contaminated sorghum biomass

2016

Presence of mycotoxins in sorghum and intake estimation in Tunisia

2014

Sorghum samples (n = 60) from Tunisian markets were analysed for the occurrence of 22 of both traditional and emerging mycotoxins. Samples were extracted with a QuEChERS-like method and mycotoxins were detected by LC-MS/MS. This method was validated and adequate analytical parameters were obtained. All samples had contamination with mycotoxins and several samples had higher contamination levels than European Union legislative limits (MLs). The most frequently found mycotoxins were ENB (100%), OTA (98%), ENA₁ (63%), ENB₁ (56%), BEA (48%), AFB1 (38%) and STG (33%). Mean contaminations were 30.7, 1.93, 33.2, 51.0, 15.4, 1.49 and 20.5 µg kg(-1), respectively. While two samples were contaminated…

Potential predictability of crop impacting climate variables for East Africa and application to sorghum in the Mt Kenya area

2013

In Southern countries with rural low income populations, the vulnerability of rainfed agriculture to rainfall variability requires effective solutions to mitigate the effects of climatic hazards on crops. Predicting the characteristics of rainy seasons some time before they start should help the establishment of agricultural adaptation strategies to rainfall hazards. This is the objective of the present study, focused on East Africa (Kenya and northern Tanzania), and divided in three parts:- Define and document intra-seasonal descriptors (ISD) that will be considered in the predictability study. A new methodological approach has been developed in order to define the onset date (ORS) and the…

Hard Cap Espresso Machine Extraction of Polyphenolic Compounds from Pulses

2018

A hard cap espresso machine was employed for the extraction of polyphenolic compounds from food samples as dry legumes and pulses. 100 mg of ground dry sample was mixed with a dispersing agent and placed in a refillable stainless steel capsule with a borosilicate filter. A complete polyphenol extraction was achieved by using 150 mL ethanol 50 % (v/v) in water and determined by Folin-Ciocalteu spectrophotometric method. The method provided a limit of detection of 0.4 mg g<sup>-1</sup>. The whole extraction procedure was achieved in less than one minute using the mild extraction conditions of 72°C and 19 bars given by the domestic machine. Fifteen varieties of food samples (3 lent…

Determination of Fusarium mycotoxins enniatins, beauvericin and fusaproliferin in cereals and derived products from Tunisia

2011

Abstract In this study, 51 samples of cereals (wheat, Barley, maize and Sorghum) and by-products (mainly pasta and couscous) purchased from Tunisian supermarkets were examined for contamination with the emerging Fusarium mycotoxins: Enniatins ENs (EN A, EN A 1 , EN B and EN B 1 ), beauvericin (BEA) and fusaproliferin (FUS). The extraction of the samples was performed with methanol using an Ultra-turrax homogenizer. Mycotoxins were analyzed with a liquid chromatography (LC) coupled to a diode array detector (DAD). The frequencies of contamination of total samples with ENs were 96%. EN A 1 was the most common EN found with the highest prevalence of 92.1%, levels ranged between 11.1 and 480 mg…

Multiple methods for the identification of polymorphic simple sequence repeats (SSRs) in sorghum [Sorghum bicolor (L.) Moench]

1995

Simple sequence repeats (SSRs), also known as microsatellites, are highly variable DNA sequences that can be used as markers for the genetic analysis of plants. Three approaches were followed for the development of PCR primers for the amplification of DNA fragments containing SSRs from sorghum [Sorghum bicolor (L.) Moench]: a search for sorghum SSRs in public DNA databases; the use of SSR-specific primers developed in the Poaceae species maize (Zea mays L.) and seashore paspalum grass (Paspalum vaginatum Swartz); and the screening of sorghum genomic libraries by hybridization with SSR oligonucleotides. A total of 49 sorghum SSR-specific PCR primer pairs (two designed from GenBank SSR-contai…

The potential of the Phytotoxkit microbiotest for hazard evaluation of sediments in eutrophic freshwater ecosystems

2009

The applicability of the Phytotoxkit microbiotest for toxicity assessment of sediments in eutrophic freshwater ecosystems was evaluated. Sediments were collected from Turawa dam reservoir (southwestern Poland) which, for years, has been subjected to a marked nutrient enrichment and heavy metal contamination. The test plant species were exposed to whole sediments, solid phases of sediments, and pore waters. Phytotoxicity was estimated on the basis of seed germination and root elongation measurements, combined into an overall germination index (GI). For pore waters, the majority of GI values were not statistically different from the controls, which was consistent with chemical data. For solid…

Comparison of the Phytotoxkit microbiotest and chemical variables for toxicity evaluation of sediments

2006

The main objective of the research was to evaluate the suitability of the Phytotoxkit microbiotest as a tool for hazard assessment of sediments. The concentrations of oil derivatives, polycyclic aromatic hydrocarbons (PAHs), and heavy metals (Cd, Cr, Cu, Hg, Ni, Pb, and Zn) were determined in sediment samples collected from the urban canal in Opole (Poland), in order to obtain a general insight of the level of sediment contamination. Phytotoxicity of sediments was estimated on the basis of seed germination and root elongation measurements, combined into an overall germination index (Gl). The results revealed spatial and vertical differentiation in sediment contamination. A good correlation …

Determining water use of sorghum from two-source energy balance and radiometric temperatures

2011

Estimates of surface actual evapotranspiration (ET) can assist in predicting crop water requirements. An alternative to the traditional crop-coefficient methods are the energy balance models. The objective of this research was to show how surface temperature observations can be used, together with a two-source energy balance model, to determine crop water use throughout the different phenological stages of a crop grown. Radiometric temperatures were collected in a sorghum (<i>Sorghum bicolor</i>) field as part of an experimental campaign carried out in Barrax, Spain, during the 2010 summer growing season. Performance of the Simplified Two-Source Energy Balance (STSEB) model was …

Determining irrigation needs of sorghum from two-source energy balance and radiometric temperatures

2011

Abstract. Estimates of surface actual evapotranspiration (ET) can assist in predicting crop water requirements. An alternative to the traditional crop-coefficient methods are the energy balance models. The objective of this research was to show how surface temperature observations can be used, together with a two-source energy balance model, to determine crop water use throughout the different phenological stages of a crop grown. Radiometric temperatures were collected in a sorghum (Sorghum bicolor) field as part of an experimental campaign carried out in Barrax, Spain, during the 2010 summer growing season. Performance of the Simplified Two-Source Energy Balance (STSEB) model was evaluated…