Search results for "Spectrophotometry"

Characterization of prehispanic cosmetics found in a burial of the ancient city of Teotihuacan (Mexico)

2012

The present paper reports the chemical data obtained on samples of pigmenting materials contained in 31 miniature vessels found in a burial found in Teopancazco, a multiethnic neighborhood center located in the southeastern sector of the archaeological site of Teotihuacan (Central Mexico) and the analytical protocol established for the complete characterization of these archaeological materials. For this purpose a multi-technique approach based on the combination of several non destructive and micro-destructive instrumental techniques, namely, light microscopy (LM), scanning electron microscopy-X-ray micro-analysis (SEMe EDX), transmission electron microscopy (TEM), voltammetry of micropart…

Integrated methodologies for qualitative and quantitative characterization of historical pigments

2014

Design and synthesis of colloidal nanocrystal heterostructures with tetrapod morphology.

2006

Determination at low ppm levels of dithiocarbamate residues in foodstuff by vapour phase-liquid phase microextraction-infrared spectroscopy

2010

Abstract A methodology based on the vapour phase (VP) generation of carbon disulphide from non-volatile dithiocarbamate compounds has been developed for determination of pesticide residues in foodstuff at low ppm levels. The method involves a selective reaction combined with liquid phase microextraction (LPME) and transmission infrared measurements. The use of a home made transmission cell improved the detection limits (LOD) compared to the use of attenuated total reflectance measurements by a factor of approximately 80. Using the most appropriate experimental conditions for the CS2 generation and LPME preconcentration, the precision of the methodology, expressed as the relative standard de…

Effect of onion consumption by rats on hepatic drug-metabolizing enzymes

2001

Fruits and vegetables or their natural constituents which increase detoxication enzymes and/or reduce activating enzymes are considered as good candidates to prevent chemically-induced carcinogenesis. In this study, rats were fed a diet supplemented with 20% onion powder for 9 days. Several cytochrome P450 (CYP)s enzymes (CYP 1A, 2B, 2E1, 3A), which are involved in carcinogen activation, were determined by measuring their enzyme activities using specific substrates. In addition, phase II enzymes activities such as UDP-glucuronosyltransferase (UGT) and glutathione S-transferase (GST), involved in detoxication of carcinogens, were measured. Protein levels of CYPs and GST A1/A2, A3/A5, Ml, M2 …

Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria

2013

2 figuras, 2 tablas

Connection between Absorption Properties and Conformational Changes in Deinococcus radiodurans Phytochrome

2014

Phytochromes consist of several protein domains and a linear tetrapyrrole molecule, which interact as a red-light-sensing system. In this study, size-exclusion chromatography and light-scattering techniques are combined with UV-vis spectroscopy to investigate light-induced changes in dimeric Deinococcus radiodurans bacterial phytochrome (DrBphP) and its subdomains. The photosensory unit (DrCBD-PHY) shows an unusually stable Pfr state with minimal dark reversion, whereas the histidine kinase (HK) domain facilitates dark reversion to the resting state. Size-exclusion chromatography reveals that all phytochrome fragments remain as dimers in the illuminated state and dark state. Still, the elut…

Simultaneous Color Contrast in Goldfish— a Quantitative Study

1997

AbstractA set of 9–15 colored test fields was presented to goldfish. In Experiment 1, test field hues ranged from green through yellow to red; in Experiment 2, the hues varied from blue through gray to yellow. In the training conditions, the test fields were presented with a gray or black surround. The fish learned to choose one intermediate test field hue by rewarding them with food. In the test conditions, the color of the surround was changed from gray to green, or red (Experiment 1), and from black to blue, or yellow (Experiment 2). The choice behavior of the goldfish changed substantially: one of the test fields other than the training test field was preferred. Direction and strength o…

Pseudopeptidic ligands: exploring the self-assembly of isophthaloylbisglycine (H2IBG) and divalent metal ions.

2012

We present a systematic study of the complexation of the new pseudopeptidic ligand isophthaloylbisglycine (H(2)IBG) with divalent metal ions of varying ionic radius. This work represents the initial employment of H(2)IBG in the coordination chemistry of alkaline earth, 3d transition, Zn(II) and Cd(II) metal elements. Infrared, NMR, thermal, magnetic, adsorption and theoretical studies of these compounds are also discussed.

Sequestering Ability of Aminopolycarboxylic (APCs) and Aminopolyphosphonic (APPs) Ligands Toward Palladium(II) in Aqueous Solution

2014

The binding capacity of three aminopolycarboxylates [nitrilotriacetic acid (NTA), ethylene-glycol-bis(2-aminoethyl ether)-N,N,N,N-tetraacetic acid (EGTA), and diethylenetriamine-N,N,N,NN-pentaacetic acid (DTPA)] and two aminopolyphosphonates {(1-hydroxyethane-1,1-diyl)bis(phosphonic acid) (HEDP) and [[(phosphonomethyl)imino]bis[2,1-ethanediylnitrilobis(methylene)]] tetrakis-phosphonic acid (DTPP)} toward palladium(II) ion was studied by potentiometric and spectrophotometric titrations at different temperatures (283.15 ≤ T/K ≤ 318.15) and ionic strengths (0.1 ≤ I/mol·dm -3 ≤ 1.0) in NaClO4. The hydrolysis of Pd2+ and the protonation of ligands were always taken into account in the speciation…