Search results for "Stabilization"

Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiae.

2009

Hyperosmotic stress yields reprogramming of gene expression in Saccharomyces cerevisiae cells. Most of this response is orchestrated by Hog1, a stress-activated, mitogen-activated protein kinase (MAPK) homologous to human p38. We investigated, on a genomic scale, the contribution of changes in transcription rates and mRNA stabilities to the modulation of mRNA amounts during the response to osmotic stress in wild-type and hog1 mutant cells. Mild osmotic shock induces a broad mRNA destabilization; however, osmo-mRNAs are up-regulated by increasing both transcription rates and mRNA half-lives. In contrast, mild or severe osmotic stress in hog1 mutants, or severe osmotic stress in wild-type cel…

DEALING WITH RISK IN AGRICULTURE: A CROP LEVEL ANALYSIS AND MANAGEMENT PROPOSAL FOR ITALIAN FARMS

2020

Risk management plays a critical role in agriculture, which is particularly exposed to multiple and heterogeneous risk factors. In addition to the traditional basic risks that generally characterize any business venture, agriculture faces external factors, generally difficult to control and with a strong impact on farm profitability. These are firstly environmental (pests and diseases) and climatic conditions that affect the quantity and quality of agricultural production, but also the structural constraints of the agricultural market, which is characterised by a high degree of supply rigidity, price volatility and inelasticity of demand. This leads to the need to implement risk management …

Multi-functional hybrid-carbon nanotubes for advanced Ultra-High Molecular Weight Polyethylene-based nanocomposites: a novel approach for stabilizati…

2015

Our results demonstrate that immobilizing stabilizing molecules on nanoparticles, and using the latter as multifunctional filler for nanocomposites, is a viable route towards to produce high-performance polymeric materials

Experimental and theoretical characterization of the strong effects on DNA stability caused by half-sandwich Ru(II) and Ir(III) bearing thiabendazole…

2020

Abstract: The synthesis and characterization of two half-sandwich complexes of Ru(II) and Ir(III) with thiabendazole as ancillary ligand and their DNA binding ability were investigated using experimental and computational methods. 1H NMR and acid–base studies have shown that aquo-complexes are the reactive species. Kinetic studies show that both complexes bind covalently to DNA through the metal site and non covalently through the ancillary ligand. Thermal stability studies, viscosity, circular dichroism measurements and quantum chemical calculations have shown that the covalent binding causes breaking of the H-bonding between base pairs, bringing about DNA denaturation and compaction. Addi…

Bovine Serum Albumin protofibril-like aggregates formation: Solo but not simple mechanism

2011

We report an experimental study on the model protein Bovine Serum Albumin (BSA), with the aim of elucidating the mechanisms by which a fully folded globular protein undergoes different aggregation pathways leading to the formation of amyloid fibrils or amorphous aggregates. We observe thermally induced formation of fibrillar structures at pH far from the protein isoelectric point. The increase of electrostatic repulsion results in protein destabilization and in modifications of inter and intra-molecular interactions leading to the growth of fibril-like aggregates stabilized by inter-molecular-β sheets. The aggregation kinetics is studied by means of fluorescence techniques, light scattering…

Experiencing with electronic image stabilization and PRNU through scene content image registration

2021

Abstract This paper explores content-based image registration as a means of dealing with and understanding better Electronic Image Stabilization (EIS) in the context of Photo Response Non-Uniformity (PRNU) alignment. A novel and robust solution to extrapolate the transformation relating the different image output formats for a given device model is proposed. This general approach can be adapted to specifically extract the scale factor (and, when appropriate, the translation) so as to align native resolution images to video frames, with or without EIS on, and proceed to compare PRNU patterns. Comparative evaluations show that the proposed approach outperforms those based on brute-force and p…

Comparative life-cycle assessment of conventional (double lane) and non-conventional (turbo and flower) roundabout intersections

2016

Abstract This research studied and compared different construction techniques for the road subgrade, embankment and pavement of different types of roundabout intersections in order to assess their environmental sustainability. A Life Cycle Assessment (LCA) was carried out on double lane, turbo- and flower roundabouts. We considered virgin materials and reclaimed asphalt pavement (RAP) for the pavement construction. Also the environmental effects due to in situ lime stabilization of fine-grained soils were assessed in order to reduce the use of virgin material in road subgrades. The use of reclaimed asphalt pavement (RAP) can lead to a significant reduction in pollutant emissions and energy …

Investigation of the magnetic field fluctuation and implementation of a temperature and pressure stabilization at SHIPTRAP

2011

Abstract Penning traps have proven to be powerful tools for the determination of nuclear masses with high accuracy. A crucial parameter for precision mass measurements in Penning traps is the accurate determination of the magnetic-field strength. However, the magnetic field of a superconducting magnet is not constant in time, but changes due to intrinsic effects of the solenoid and external perturbations. These effects have been investigated for SHIPTRAP. Furthermore, a stabilization of the temperature in the magnet bore as well as of the pressure in the liquid-helium cryostat has been implemented. Thus, the magnetic-field related uncertainties have been reduced to 7(6)×10−11/h.

Degradation and stabilization of the polyolefins and of the filled-polyolefins

2009

From the great depression to bretton woods: Jacob Viner and international monetary stabilization (1930-1945)

2009

This paper examines Jacob Viner's contribution to the debate and the policy decision-making concerning international monetary policy from the Great Depression to the Bretton Woods agreements. An outstanding member of the so-called 'early Chicago School of Political Economy', Viner was actively engaged in the debate over the causes and cures of the Depression, emphasizing the important role international economic problems played in producing its onset and in reinforcing its duration. During the 1930s Viner was an outspoken supporter of international monetary cooperation, set up to secure exchange rates stability, which he regarded as a paramount factor in restoring business confidence and fo…