Search results for "Staphylococci"

showing 3 items of 13 documents

ANTIMICROBIAL AND ANTISTAPHYLOCOCCAL BIOFILM ACTIVITY FROM THE SEA URCHIN PARACENTROTUS LIVIDUS

2009

Aims: Staphylococcal biofilm-associated infections are resistant to conventional antibiotics. Consequently, new agents are needed to treat them. With this aim, we focused on the effector cells (coelomocytes) of the sea urchin Paracentrotus lividus immune system. Methods and Results: We tested the activity of the 5-kDa peptide fraction of the cytosol from coelomocytes (5-CC) against a group of Gram-positive, Gram-negative bacteria and fungi. We determined minimal inhibitory concentrations (MICs) ranging from 253.7 to 15.8 mg ml(-1). We observed an inhibitory activity and antibiofilm properties of 5-CC against staphylococcal biofilms of reference strains Staphylococcus epidermidis DSM 3269 an…

Staphylococcus aureusMicrobial ViabilityMicroscopy ConfocalStaining and LabelingMicrobial Sensitivity TestsStaphylococcal InfectionsCell FractionationSettore BIO/19 - Microbiologia GeneraleThymosinCytosolAnti-Infective AgentsBiofilmsParacentrotusStaphylococcus epidermidisAnimalsPeptidesantimicrobial antimicrobial peptides biofilminnate immunity staphylococci
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Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa…

2014

The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics. Many antimicrobial peptides show a high specificity for prokaryotes and a low toxicity for eukaryotic cells and, due to their mode of action the development of resistance is considered unlikely. We recently characterized an antimicrobial peptide that was called Paracentrin 1 from the 5-kDa peptide fraction from the coelomocyte cytosol of the Paracentrotus lividus. In this study, the chemically synthesized Paracentrin 1, was tested for its antimicrobial and antibiof…

medicine.drug_classAMP (Antimicrobial peptides); Biofilm; Staphylococci; Pseudomonas aeruginosa; Paracentrotus lividusAntibioticsAntimicrobial peptidesBiophysicsSettore BIO/05 - ZoologiaPeptideBiologymedicine.disease_causeSettore BIO/19 - Microbiologia GeneraleApplied Microbiology and BiotechnologyParacentrotus lividusMicrobiologymedicineMode of actionAMP (Antimicrobial peptides)Staphylococcichemistry.chemical_classificationPseudomonas aeruginosaBiofilmBiofilmAntimicrobialbiology.organism_classificationchemistryParacentrotus lividusSettore CHIM/03 - Chimica Generale E InorganicaPseudomonas aeruginosaOriginal Article
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Staphylococcus epidermidis virulences faktori un epidemioloģiskā nozīme

2016

Staphylococcus epidermidis svarīgākais virule nces faktors ir biofilmas veidošana. Lai diferencētu komensālos celmus no kolonizējošiem un invazīviem celmiem, pētījumā tika izmantotas fenotipiskās metodes, nosakot biofilmas veidošanu, antibiogrammu, un molekulārās bioloģijas metodes, nosakot virulences gēnus aap / ica A un mec A. Rezultāti liecina, ka biofilmu veidošana raksturīga klīniskajiem celmiem, statistiski ticami biežāk gēni aap/ica A bija noteikti klīniskajos S.epidermidis celmos, tie vis biežāk bija rezistenti pret meticilīnu (97,8%). S.epidermidis tipēšanai pirmo reizi tika pielietota MLST (multilokusu sekvenču tipēšana) metode. Pētījuma rezultāti ļauj secināt par S.epidermidis ge…

meticilīnrezistencemethicillin resistancegenotypingvirulence factorsMedicinegenotipēšanaS.epidermidisVeselības aprūpeMedicīnastaphylococci
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