Search results for "Sterols"

7keto-stigmasterol and 7keto-cholesterol induce differential proteome changes to intestinal epitelial (Caco-2) cells

2015

Abstract Recent studies have expanded the appreciation of the roles of oxysterols triggering inflammatory, immune cytotoxic and apoptotic processes, but have not been considered for proteome analysis. A comparative proteomic study in intestinal epithelial cell cultures incubated (60 μM/24 h) with 7keto-cholesterol or 7keto-stigmasterol was performed. The influence of both compounds was studied following the nLC-TripleTOF analysis. Findings were compared to results for control cultures. In the principal component analysis (PCA) of proteome patterns, two components were extracted accounting for 99.8% of the variance in the protein expression. PCA analysis clearly discriminated between the per…

Versatile, sensitive liquid chromatography mass spectrometry – Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins

2016

AbstractWe have designed a versatile and sensitive liquid chromatographic (LC) system, featuring a monolithic trap column and a very narrow (10 μm ID) fused silica open tubular liquid chromatography (OTLC) separation column functionalized with C18-groups, for separating a wide range of molecules (from small metabolites to intact proteins). Compared to today’s capillary/nanoLC approaches, our system provides significantly enhanced sensitivity (up to several orders) with matching or improved separation efficiency, and highly repeatable chromatographic performance. The chemical properties of the trap column and the analytical column were fine-tuned to obtain practical sample loading capacities…

Statīnu lietošana kardiovaskulāro traucējumu gadījumos

2018

Paaugstinātu holesterola līmeni uzskata par vienu no biežākajiem kardiovaskulāro slimību cēloņiem, padarot statīnus biežāk lietotajiem medikamentiem. Konstatēts, ka statīni izraisa muskuļu bojājumus, paaugstinātu glikozes līmeni, gastrointestinālos un kognitīvos traucējumus, aknu un nieru bojājumus, taču 74,49 % gadījumos pacienti par to nav brīdināti. Maģistra darba mērķis ir apkopot statīnu lietotāju sniegto informāciju par medikamentu radītajām blakusparādībām un izpētīt to apriti “Mēness aptiekā 47”, salīdzināt 2007. un 2017.gadā E.Gulbja laboratorijā veikto analīžu rezultātus. Dati liecina, ka ārsti biežāk izraksta atorvastatīnu un rosuvastatīnu 20 mg devā, turklāt atorvastatīna lietoš…

Fatty acids, sterols and antioxidant compounds of minor and neglected cultivar of Sicilian virgin olive oils

2007

Acidic and sterol composition, pigment fraction and antioxidant compounds (tocopherols, total phenols and polar phenols) from eight monovarietal Sicilian olive oil samples, analyzed during the crop seasons from 2003/2004 to 2005/2006, are reported. Cultivars were divided, according to their diffusion, in main, minor and neglected cultivars. Encouraging data were observed for minor and neglected cultivars, for some revaluation in regard to their relevance, because they showed the highest content in tocopherols, in total phenols and polar phenols compared with main cultivars.

HPLC PROFILE AND EVOLUTION DURING STORAGE OF TRIGLYCERIDES AND DIGLYCERIDES IN VIRGIN OLIVE OILS

2007

Acidic and sterol composition, pigment fraction and antioxidant compounds (tocopherols, total phenols and polar phenols) from eight monovarietal Sicilian olive oil samples, analyzed during the crop seasons from 2003/2004 to 2005/2006, are reported. Cultivars were divided, according to their diffusion, in main, minor and neglected cultivars. Encouraging data were observed for minor and neglected cultivars, for some revaluation in regard to their relevance, because they showed the highest content in tocopherols, in total phenols and polar phenols compared with main cultivars.

The diet phytochemical Indicaxanthin suppresses 7-ketocholesterol-induced THP-1 cell apoptosis via inhibition of redox unbalance, cytosolic Ca2+ incr…

2012

Analysis of sterols by high-performance liquid chromatography/mass spectrometry combined with chemometrics

2006

A newly developed high-performance liquid chromatography/mass spectrometry (HPLC/MS) method has been successfully used to analyze plasma concentrations of various phytosterols (cholestanol and beta-sitosterol) and cholesterol metabolites (desmosterol and lathosterol). This was based on an unusual solvent combination of water/methanol vs. methanol/acetone/n-hexane applied on a Purospher Star RP-18e (125 x 2 mm, 3 microm) column, which proved excellent for the separation, identification and quantification of plasma sterols. Simple solid-phase extraction preparation of plasma samples was performed, followed by the developed fast and robust HPLC separation. Results on four groups of people were…

Steroidal saponins from Chlorophytum deistelianum.

2016

Abstract Phytochemical investigation of the aerial parts of Chlorophytum deistelianum led to the isolation of four previously undescribed steroidal saponins called chlorodeistelianosides A–D with five known ones. Their structures were established mainly by extensive 1D and 2D NMR spectroscopic techniques and mass spectrometry as (25R)-3β-[(β- d -glucopyranosyl-(1 → 3)-[α- l -rhamnopyranosyl-(1 → 4)]-β- d -xylopyranosyl-(1 → 3)-[β- d -glucopyranosyl-(1 → 2)]-β- d -glucopyranosyl-(1 → 4)-β- d -galactopyranosyl)oxy]-5α-spirostan-12-one, (24S,25S)-24-[(β- d -glucopyranosyl)oxy]-3β-[(β- d -glucopyranosyl-(1 → 2)-[β- d -xylopyranosyl-(1 → 3)]-β- d -glucopyranosyl-(1 → 4)-β- d -galactopyranosyl)ox…

Contribution à l’étude de la fraction insaponifiable de trois huiles d’olive issues des variétés Guasto, Rougette et Blanquette plantés dans l’est al…

2010

The sterol profile of three Algerian virgin olive oils produced from Guasto, Blanquette and Rougette cultivar’s, varieties grown in the East of the country, was established by gas chromatography using a flame ionization detector and coupled to mass spectrometry. Four sterol compounds were identified and characterized in the three oils which are, sitosterol always predominant (43-66%), delta 5-avenasterol (8-13%), stigmasterol (≤ 1,5%) and campesterol (1-2%); one methyl sterol (4-methylsterol) : citrostadienol (4-7%) and two triterpenic alcohols : 24-methylene cycloartanol (10-33%) and cycloartenol (3-13%). These results vary according to campany and variety. Our study shows that most of the…

Sterol Composition in Infant Formulas and Estimated Intake.

2015

Sterol contents in infant formulas (IFs) from the European market were determined, and their intakes by infants between 0 and 6 months were evaluated. Total animal sterols (mg/100 mL) ranged from 1.71 to 5.46, cholesterol being the main animal sterol (1.46-5.1). In general, cholesterol and desmosterol were lower than the human milk (HM) values indicated by other authors. Total plant sterol (mg/100 mL) ranged from 3.1 to 5.0. β-Sitosterol, the most abundant phytosterol, ranged from 1.82 to 3.01, followed by campesterol (0.72-1.15), stigmasterol (0.27-0.53), and brassicasterol (0.14-0.28). Cholesterol intake (mg/day) ranged from 9 to 51 and plant sterol intake (mg/day) from 19 to 50. The ster…