Search results for "Streptococcus"
showing 10 items of 169 documents
Immune response to the 23-valent polysaccharide pneumococcal vaccine after the 7-valent conjugate vaccine in allogeneic stem cell transplant recipien…
2009
The current recommendations for active immunization after stem cell transplant (SCT) include 3 doses of 7-valent pneumococcal conjugate vaccine (PCV7) from 3 months after transplant, followed by a 23-valent polysaccharide pneumococcal vaccine (PPV23). However, until now, the immune response to PPV23 after PCV7 has not been assessed after SCT. In the EBMT IDWP01 trial, 101 patients received 1 dose of PPV23 at 12 or 18 months, both after 3 doses of PCV7. The efficacy of PPV23 was assessed 1 month later and at 24 months after transplant by the pneumococcal serotype 1 and 5 antibody levels. Serotype 1 and 5 are not included in PCV7. Although the geometric mean concentrations were significantly …
Streptococcus agalactiae and Chlamydia trachomatis detection in women without symptoms of infection.
2020
Background Chlamydia trachomatis (C. trachomatis) and Streptococcus agalactiae (GBS) may be present in the female cervical canal without any symptoms of infection. Chronic chlamydial infections lead to many serious complications and perinatal infections, while the presence of GBS is a reservoir for infections of newborns or invasive streptococcal infection in adults. Objectives To examine healthy women for C. trachomatis without symptoms from the reproductive system, assess the frequency of asymptomatic infections, detect GBS in the cervical canal, demonstrate differences in drug susceptibility, and determine the serotype of S. agalactiae strains and correlations among the ones present in t…
Acidic Monosaccharides become Incorporated into Calcite Single Crystals*.
2020
Streptococcus suis is an encapsulated bacterium and one of the most important swine pathogens and a zoonotic agent for which no effective vaccine exists. Bacterial capsular polysaccharides (CPSs) are poorly immunogenic, but anti-CPS antibodies are essential to the host defense against encapsulated bacteria. In addition to the previously known serotypes 2 and 14, that are non-immunogenic, we have recently purified and described the CPS structures for serotypes 1, 1/2, 3, 7, 8, and 9. Here, we aimed to elucidate how these new structurally diverse CPSs interact with the immune system to generate anti-CPS antibody responses. CPS-stimulated dendritic cells produced significant levels of C–C moti…
Safety, tolerability, and immunologic noninferiority of a 13-valent pneumococcal conjugate vaccine compared to a 7-valent pneumococcal conjugate vacc…
2010
13-valent pneumococcal conjugate vaccine (PCV13) was compared to PCV7 in infants administered 4 doses. For the 7 common serotypes, PCV13- and PCV7-elicited responses showed comparable percent responders achieving 0.35mug/mL IgG threshold (exception 6B, 77.5% versus 87.1%, respectively) and OPA titers of 1:8; IgGs were lower than PCV7 but functional responses were generally comparable. For the 6 additional serotypes, PCV13-elicited IgG and functional OPA responses were notably greater than PCV7. The toddler dose boosted immune responses. Vaccines were comparable with regard to safety. PCV13 should be as effective as PCV7 in preventing pneumococcal disease caused by the common serotypes and m…
Persistence of wild Streptococcus thermophilus strains on wooden vat and during the manufacture of a traditional Caciocavallo type cheese
2012
The present work was undertaken to evaluate the influence of the wooden dairy plant equipment on the microbiological characteristics of curd to be transformed into Caciocavallo Palermitano cheese. Traditional raw milk productions were performed concomitantly with standard cheese making trials carried out in stainless steel vat inoculated with a commercial starter. Milk from two different farms (A and B) was separately processed. The wooden vat was found to be a reservoir of lactic acid bacteria (LAB), while unwanted (spoilage and/or pathogenic) microorganisms were not hosted or were present at very low levels. All microbial groups were numerically different in bulk milks, showing higher lev…
Single tube real time PCR for detection of Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila from …
2012
We designed a multiplex real time PCR for rapid, sensitive and specific detection of Streptococcus pneumoniae, Legionella pneumophila, Chlamydophila pneumoniae and Mycoplasma pneumoniae. The study cases consisted of 129 patients with community acquired pneumonia (CAP). Bacteriological techniques were implemented for detection of the cultivable organisms. DNA were extracted from sputa, throat swabs, bronchoalveolar lavages and tracheal aspirates and used as templates in real time PCR. The primers and probes were designed for cbpA (S. pneumoniae), p1adhesin (M. pneumoniae), mip (L. pneumophila) and ompA (C. pneumoniae). After optimization of real time PCR for every organism, the experiments w…
EDEMA EMORRAGICO ACUTO DELL'INFANZIA: UN CASE REPORT
2019
Un lattante di 6 mesi maschio giunge alla nostra osservazione per comparsa di lesioni maculo-papulari alle guance estesesi, in poche ore, ai quattro arti ed evolventi in manifestazioni purpuriche su cute edematosa senza segno della fovea. All’EO all’ingresso si evidenziavano inoltre: angioedema dei padiglioni auricolari, aspetto a coccarda delle lesioni aventi diametro max di 2 cm con coinvolgimento anche dello scroto, cute del tronco e mucose integre, assenza di segni neurologici. Due giorni prima del ricovero e della comparsa delle manifestazioni cutanee il piccolo aveva presentato febbre (T max 39,5°C) e faringite trattata con amoxi-clavulanato. Esami al ricovero: PCR mossa (2,3 mg/dl; V…
Streptococcus Mutans sortāzes A ekspresija, attīrīšana un raksturošana zāļu vielu skrīningam
2019
Slimības, ko izraisa patogēni, īpaši multirezistenti, piemēram, Streptococcus mutans, ir nepieciešams apkarot. Multirezistentu grampozitīvu patogēnu baktēriju transpeptidāzes - sortāzes A - mērķproteīna inhibēšana mazina patogēnu saistīšanās spēju - virulenci - un līdz ar to arī rezistenci. Bakalaura darbā Streptococcus mutans sortāze A tiek izmantota kā instruments, lai pārbaudītu novatoriskus ķīmiskus savienojumus, kam piemīt spēja inhibēt sortāzi A. Mērķis bija, izmantojot zāļu vielu skrīninga metodi, atlasīt ķīmiskas vielas, kam piemīt sortāzes A inhibēšana un kuras varētu izmantot, lai apkarotu multirezistentu grampozitīvu patogēnu izraistītas slimības. Streptococcus mutans sortāzes A …
No klīniskajiem paraugiem izdalīto Streptococcus pneumoniae virulences faktori un rezistence pret antibakteriālajiem līdzekļiem
2020
Ievads: Latvijā un Pasaulē aktuāla problēma ir Streptococcus pneumoniae baktērijas rezistence, kas izveidojusies pret antibakteriālajiem līdzekļiem. Rezistento Streptococcus pneumoniae celmu skaits palielinās ar katru gadu. Preventīvie pasākumi, kas samazinātu rezistences incidenci ir svarīga atbildīga un piesardzīga antibakteriālo līdzekļu izmantošana cilvēku veselības uzlabošanai un pacientu informētība, izpratne par Streptococcus pneumoniae rezistenci. Darba mērķi: Noteikt Streptecoccus pneumoniae jutību pret antibakteriālajiem līdzekļiem, kas iegūti no klīniskā materiāla paraugiem. Materiāli un metodes: Pētījums veikts RAKUS stacionārs ‘’Tuberkulozes un plaušu slimību centrs’’ klīnikas …
No klīniskā materiāla izdalīto Streptococcus pneumoniae jutība pret antibakteriālajiem līdzekļiem
2016
Nozīmīga sabiedrības veselības problēma ir baktēriju rezistence pret antibakteriālajiem līdzekļiem. Pasaulē parādās arvien jauni pret antibakteriālajiem līdzekļiem rezistenti S. pneumoniae celmi. Tas galvenokārt, saistīts ar antibakteriālo līdzekļu nepareizu pielietošanu un plaša spektra preparātu lietošanu, kad ir iespējams izmantot šaura spektra antibakteriālus līdzekļus. Darba mērķis: Noteikt S. pneumoniae jutību pret antibakteriālajiem līdzekļiem, celmiem, kas izdalīti no klīniskā materiāla. Darba uzdevumi: Veikt laboratorijā identificēto S. pneumoniae jutības testus pret antibakteriālajiem līdzekļiem, pielietojot BBLTM DDT un BBLTM E – testu, analizēt statistiskos datus, izmantojot Mic…