Search results for "Substrate"
showing 10 items of 1018 documents
Etude des mécanismes physiologiques et moléculaires permettant la prise en charge des substrats hydrophobes par la levure Yarrowia lipolytica au nive…
2012
Yarrowia lipolytica can grow on many hydrophobic substrates and metabolize them via the β-oxidation pathway or store them into lipid bodies. The first contact between the cell and the hydrophobic substrates is by the cell wall. In this step, cell-wall proteins (CWP), β-glucane and chitin of the cell wall could play an important role for the adhesion and uptake of hydrophobic substrates. The aim of this work was to study the effect of a hydrophobic carbon source (compared with glucose) and the culture medium composition on the cell wall composition and the melanin production by Y. lipolytica. The results of our biochemical and molecular analysis showed that the presence of methyl oleate and …
Establishment of Petunia hybrida as model for studying mycorrhiza-induced resistance
2012
Root pathogens play an important economical role in the production of vegetable and ornamental plants. They cause high losses especially in green house production and are difficult to combat by conventional methods, because the use of pesticides in soils or substrates is limited. The application of arbuscular mycorrhiza could be an alternative as mycorrhizal plants generally showed higher resistance against various soil-borne pathogenic fungi. The success of the inoculation is, however, not predictable and mechanisms behind the protective effects are largely unknown. The ornamental crop Petunia hybrida is among other solanaceous plants confronted with attack by root pathogens and was theref…
Cyclopropane Fatty Acid Synthase from Oenococcus oeni: Expression in Lactococcus lactis subsp. Cremoris and Biochemical Characterization
2015
Bacterial cyclopropane fatty acid synthases (CFA synthases) catalyze the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to the double bond of a lipid chain, thereby forming a cyclopropane ring. CFAs contribute to resistance to acidity, dryness, and osmotic imbalance in many bacteria. This work describes the first biochemical characterization of a lactic acid bacterium CFA synthase. We have overexpressed Oenococcus oeni CFA synthase in E. coli in order to purify the enzyme. The optimum cyclopropanation activity was obtained at pH 5.6 and 35.8 °C. The high K(m) (AdoMet) value obtained (2.26 mM) demonstrates the low affinity of O. oeni enzyme toward the L. lactis subsp. cremo…
Guidance of surface elastic waves along a linear chain of pillars
2016
International audience; The propagation of surface elastic waves, or surface phonons, is considered along a linear and periodic chain of cylindrical pillars sitting on a semi-infinite solid substrate. A variety of guided modes, some of them exhibiting a very low group velocity, are shown to exist at frequencies close to the resonance frequencies of the pillars. Although the pillar diameter is typically smaller than half the relevant wavelength, lateral radiation on the surface is found to be canceled. Surface guidance is explained by the hybridization of the resonating pillars with the continuum of elastic waves of the substrate.
Development of Removable Visual Impact Indicator or Polymer Composite Materials
2021
The prototype of removable visual impact indicator for thermoset polymer composite materials is developed, and its characteristics are experimentally determined. The indicator is a fabric tape glued by epoxy to the surface of the polymer composite. The tape is impregnated with a composition that provides a visual response at the place of an impact on the composite surface. Ball-drop tests demonstrated the increase of the magnitude of the visual response with the impact energy at different substrate hardnesses. The shelf-life and mode of the tape storage until commissioning are determined. Peel tests showed the ability to remove a used indicator without damaging the surface of the composite.
Biochemical and structural features of a novel cyclodextrinase from cow rumen metagenome.
2007
A novel enzyme, RA.04, belonging to the alpha-amylase family was obtained after expression of metagenomic DNA from rumen fluid (Ferrer et al.: Environ. Microbiol. 2005, 7, 1996-2010). The purified RA.04 has a tetrameric structure (280 kDa) and exhibited maximum activity (5000 U/mg protein) at 70 degrees C and was active within an unusually broad pH range from 5.5 to 9.0. It maintained 80% activity at pH 5.0 and 9.5 and 75 degrees C. The enzyme hydrolyzed alpha-D-(1,4) bonds 13-fold faster than alpha-D-(1,6) bonds to yield maltose and glucose as the main products, and it exhibited transglycosylation activity. Its preferred substrates, in the descending order, were maltooligosaccharides (C3-C…
Compost di qualità per la coltivazione del peperoncino ornamentale in vaso
2010
In two years (2008 and 2009), the research centre Co.Ri.SSIA evalueted the suitability of compost obtained from organic municipal waste used a growth substrate for three varieties of ornamental chili pepper (Capsicum annuum). Research was carried out at the Orleans Experimental Farm at the University of Palermo. In addition to the control standard substrate (a 70:30 mix by volume of peat and perlite), three substrates were tested, formed by varying percentage mixes of compost, peat and perlite. During the initial cultivation stage, chemical and physical analyses were carried out on the different substrates. Subsequently, morphometric data were collected in order to evaluate the plant orname…
Biochemical methane potential (BMP) of solid organic substrates: evaluation of anaerobic biodegradability using data from an international interlabor…
2011
Background: This paper describes results obtained for different participating research groups in an interlaboratory study related to biochemical methane potential (BMP). In this research work, all experimental conditions influencing the test such as inoculum, substrate characteristics and experimental conditions were investigated. The study was performed using four substrates: three positive control substrates (starch, cellulose and gelatine), and one raw biomass material (mung bean) at two different inoculum to substrate ratios (ISR). Results: The average methane yields for starch, cellulose, gelatine and mung bean at ISR of 2 and 1 were 350 ± 33, 350 ± 29, 380 ± 42, 370 ± 36 and 370 ± 35 …
Energy transfer between polyamine chains bearing naphthalene terminal units and k3[Co(CN)6]: An example of a molecular photoreactor
2002
Molecular photoreactors consisting of polyamine chains (receptors) bearing terminal naphthalene units (antennae) are described. The receptors are used to bind the substrate hexacyanocobaltate(III) and the antennae to transfer energy to the complex and thus promote a photoaquation reaction.
A novel TiO2-assisted magnetic nanoparticle separator for treatment and inactivation of bacterial contaminants in aquatic systems
2014
Ferromagnetic nanoparticles (Fe-nanoparticles) have been functionalized with recombinant poly- Glu [glutamic acid]-tagged silicatein, a biomineral-synthesizing enzyme from siliceous sponges that forms the inorganic silica skeleton of those animals. The biocatalytic activity of silicatein was used to form a titania (TiO2) shell around the iron nanoparticle core, using the water-soluble non- natural substrate titanium bis(ammonium lactato)-dihydroxide (TiBALDH). Thereby the diameter of the nanoparticles increases from 7 nm to ≈22 nm. This procedure also allows the layer-by-layer fabrication of titania/silica- Fe-nanoparticles. SEM/EDX analysis confirmed the presence of the Ti and Si signals i…