Search results for "Swine."
showing 10 items of 466 documents
Toward morphological thoracic EIT: major signal sources correspond to respective organ locations in CT.
2012
Lung and cardiovascular monitoring applications of electrical impedance tomography (EIT) require localization of relevant functional structures or organs of interest within the reconstructed images. We describe an algorithm for automatic detection of heart and lung regions in a time series of EIT images. Using EIT reconstruction based on anatomical models, candidate regions are identified in the frequency domain and image-based classification techniques applied. The algorithm was validated on a set of simultaneously recorded EIT and CT data in pigs. In all cases, identified regions in EIT images corresponded to those manually segmented in the matched CT image. Results demonstrate the abilit…
Recovering coronavirus from large volumes of water
2021
The need for monitoring tools to better control the ongoing coronavirus disease (COVID-19) pandemic is extremely urgent and the contamination of water resources by excreted viral particles poses alarming questions to be answered. As a first step to overcome technical limitations in monitoring SARS-CoV-2 along the water cycle, we assessed the analytical performance of a dead end hollow fiber ultrafiltration coupled to different options for secondary concentrations to concentrate viral particles from large volume of spiked tap water, seawater and surface water together with two quantitative RT-qPCR detection kits. Spiking the porcine epidemic diarrhea virus (PEDV), an enveloped virus surrogat…
A influência do método de armazenamento na transparência do cristalino de porco
2019
Este artículo se ha publicado de forma definitiva en: http://www.scielo.br/pdf/abo/v82n1/0004-2749-abo-82-01-0056.pdf Purpose: The porcine eye is frequently used as a research model. This paper analyzes the effect of different storage methods on the transparency of pig crystalline lens. Methods: A spectral transmission curve (from 220 to 780 nm) for the crystalline lens was determined experimentally after storage in different conditions: saline solution, formalin, castor oil, and freezing at -80°C. The total transmission in the visible spectrum, which was used as an index of transparency, was calculated from these curves. For comparative purposes, fresh lenses were evaluated and used as con…
Vitrification of Immature Porcine Oocytes: Effects of Lipid Droplets, Temperature, Cytoskeleton, and Addition and Removal of Cryoprotectant
1998
Three experiments were conducted to investigate the effects of single-step and stepwise exposure to and removal of cryoprotectant, of temperature, and of a cytoskeletal relaxant on the development of germinal vesicle porcine oocytes to the M-II stage. In experiment I, noncooled cumulus-oocyte complexes (COCs) were treated using single-step/stepwise exposure to ethylene glycol (EG) and removal at 23 or 42 degrees C. Stepwise exposure to EG and dilution at 42 degrees C were found to have a positive effect on the COC developmental rate. In experiment II, also without cooling, COCs were treated with Cytochalasin B at 42 degrees C using single-step and stepwise protocols of exposure to and remov…
A comparative study of mechanical properties of fresh and frozen-thawed porcine intervertebral discs in a bioreactor environment
2016
Abstract Limited availability of fresh intervertebral discs (IVDs) necessitates the use of frozen-thawed samples in biomechanical investigations. Several authors have reported the mechanical properties of frozen-thawed IVDs, but few studies have investigated the effects of storage temperature and cooling rate. The validity and reliability of the applied freezing-thawing procedures are still relatively unknown. The study aims to compare the mechanical responses of fresh porcine IVDs and porcine IVDs, which were frozen at different freezing temperatures with a controlled-rate cooling process and then thawed. The specimens were obtained from pigs aged 6 months. A total of 18 functional spine u…
Epidemiology ofSalmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources
1993
SUMMARYSalmonella typhimuriumis the most frequently identified serovar ofSalmonellain Italy. This serovar is characterized by the widespread dissemination among human and non-human sources of phenotypically and genetically well-differentiated clones.In this study 457 strains ofS. typhimuriumisolated in Italy in the years 1982–91 from human and animal sources were submitted to characterization by the rDNA fingerprinting technique. Application of this typing method, after digestion of chromosomal DNA withHincII endonuclease, confirmed the greatest genetic differentiation of clones ofS. typhimurium, allowing reliable identification of 45 rDNA patterns linked into 9 major clusters. rDNA pattern…
Simultaneous detection of Carnobacterium and Leuconostoc in meat products by multiplex PCR.
2004
M.C. MACIAN, E. CHENOLL AND R. AZNAR. 2004. Aims: To develop a multiplex PCR approach for simultaneous detection of Leuconostoc and Carnobacterium and its validation in meat products. Methods and Results: Two multiplex PCR assays were developed using newly designed 16S rDNA-directed primers adapted to the current taxonomic situation of genera Leuconostoc and Carnobacterium that allow: (i) simultaneous detection of both genera, and members of the nonmotile species of genus Carnobacterium and (ii) identification in a single assay of the nonmotile species C. divergens, C. maltaromicum and C. gallinarum. Sensitivity values of 10 3 and 10 4 CFU g )1 were determined for multiplex PCR detection of…
Lactic acid bacteria associated with vacuum-packed cooked meat product spoilage: population analysis by rDNA-based methods.
2007
Aim: To determine the lactic acid bacteria (LAB) implicated in bloating spoilage of vacuum-packed and refrigerated meat products. Methods and Results: A total of 18 samples corresponding to four types of meat products, with and without spoilage symptoms, were studied. In all, 387 colonies growing on de Man, Rogosa and Sharpe, yeast glucose lactose peptone and trypticase soy yeast extract plates were identified by internal spacer region (ISR), ISR-restriction fragment length polymorphism and rapid amplified ribosomal DNA restriction analysis profiles as Lactobacillus (37%), Leuconostoc (43%), Carnobacterium (11%), Enterococcus (4%) and Lactococcus (2%). Leuconostoc mesenteroides dominated …
Comparison of conventional twist drill protocol and piezosurgery for implant insertion: an ex vivo study on different bone types.
2015
Objective The aim of this ex vivo study was to compare implant insertion procedures using piezosurgery and conventional drilling in different qualities of bone. Implant bed preparation time, generated heat, and primary implant stability were analyzed. Material and methods Fresh ex vivo porcine bone block samples (cancellous, mixed, and cortical bone) were obtained. The bone quality was quantified by ultrasound transmission velocity (UTV). Each bone sample received three implants of the same diameter using each of the techniques of piezosurgery and conventional twist drills. Time for preparation was taken and the temperature while performing the osteotomy was measured using infrared spectros…
High-performance liquid chromatographic determination of sumatriptan after in vitro transdermal diffusion studies.
2004
A simple, accurate, precise and rapid HPLC method with UV detection has been validated in order to determine the in vitro transdermal absorption of sumatriptan succinate. The HPLC method is a modification of that described by Nozal et al. [M.J. Nozal, J.L. Bernal, L. Toribio, M.T. Martin, F.J. Diez, J. Pharm. Biomed. Anal. 30 (2002) 285-291]. Separation was carried out on a 250 mm Kromasil C18 column at room temperature. The detector response, at 282.7 nm, was found to be linear in a concentration range between 0.145 and 145 microM. The limit of detection (LOD) was 0.019 microM and the limit of quantification (LOQ) was 0.145 microM.