Search results for "Synthetic"
showing 10 items of 723 documents
Metabolism of third generation synthetic cannabinoids using zebrafish larvae.
2021
Synthetic cannabinoids are the second largest group of new psychoactive substances reported by the United Nations Office on Drugs and Crime in the last decade and case reports bring attention to its high potency effects and its severe toxicity, including fatalities. Moreover, synthetic cannabinoids are usually entirely metabolized and metabolic pathways for many new generation synthetic cannabinoids are still unknown. In this study, the metabolism of five third generation synthetic cannabinoids were evaluated using zebrafish (Danio rerio) larvae as 24-hours in vivo model studied within 5 days after fertilization. The studied synthetic cannabinoids were MMB-CHMICA, ADB-CHMICA, ADB-CHMINACA, …
Microbial Diversity in the Midguts of Field and Lab-Reared Populations of the European Corn Borer Ostrinia nubilalis
2011
Background: Insects are associated with microorganisms that contribute to the digestion and processing of nutrients. The European Corn Borer (ECB) is a moth present world-wide, causing severe economical damage as a pest on corn and other crops. In the present work, we give a detailed view of the complexity of the microorganisms forming the ECB midgut microbiota with the objective of comparing the biodiversity of the midgut-associated microbiota and explore their potential as a source of genes and enzymes with biotechnological applications. Methodological/Principal Findings: A high-throughput sequencing approach has been used to identify bacterial species, genes and metabolic pathways, parti…
Insertion of light-harvesting chlorophyll a/b protein into the thylakoid
2000
The major light-harvesting chlorophyll a/b-binding protein (Lhcb1,2) of photosystem II is inserted into the thylakoid via the signal recognition particle dependent pathway. However, the mechanism by which the protein enters the membrane is at this time unknown. In order to define some topographical restrictions for this process, we constructed several recombinant derivatives of Lhcb1 carrying hexahistidine tags at either protein terminus or in the stromal loop domain. Additionally, green fluorescent protein (GFP) was fused to either terminus. None of the modifications significantly impair the pigment-binding properties of the protein in the in vitro reconstitution of LHCII. With the excepti…
Down-Scaling Modis Vegetation Products with Landsat GAP Filled Surface Reflectance in Google Earth Engine
2020
High spatial resolution vegetation products are fundamental in different fields, such as improving the understanding of crop seasonality at regional scales. Here, two new vegetation products such as the Leaf Area Index (LAI) and the Fraction of Absorbed Photosynthetically Active Radiation (FAPAR) are downscaled at continental scales. A novel HIghly Scalable Temporal Adaptive Reflectance Fusion Model (HIS-TARFM) is used to generate the gap-free time series of Landsat surface reflectance data by fusing MODIS and Landsat reflectance for the contiguous United States. An artificial neural network is trained to capture the relationship between the gap free Landsat surface reflectance and the MODI…
Urban monitoring using multi-temporal SAR and multi-spectral data
2006
In some key operational domains, the joint use of synthetic aperture radar (SAR) and multi-spectral sensors has shown to be a powerful tool for Earth observation. In this paper, we analyze the potentialities of combining interferometric SAR and multi-spectral data for urban area characterization and monitoring. This study is carried out following a standard multi-source processing chain. First, a pre-processing stage is performed taking into account the underlying physics, geometry, and statistical models for the data from each sensor. Second, two different methodologies, one for supervised and another for unsupervised approaches, are followed to obtain features that optimize the urban rela…
An overview on the recent developments of 1,2,4-triazine derivatives as anticancer compounds
2017
The synthesis, the antitumor activity, the SAR and, whenever described, the possible mode of action of 1,2,4-triazine derivatives, their N-oxides, N,. N'-dioxides as well as the benzo- and hetero-fused systems are reported. Herein are treated derivatives disclosed to literature from the beginning of this century up to 2016. Among the three possible triazine isomers, 1,2,4-triazines are the most studied ones and many derivatives having remarkable antitumor activity have been reported in the literature and also patented reaching advanced phases of clinical trials.
Oral immunization with HCV-NS3-transformed Salmonella: induction of HCV-specific CTL in a transgenic mouse model.
2001
Abstract Background & Aims: The ability to induce cytotoxic T cells is considered an important feature of a candidate hepatitis C virus (HCV) vaccine. We used an oral immunization strategy with attenuated HCV-NS3–transformed Salmonella typhimurium to deliver DNA directly to the gut-associated lymphoid tissue. Methods: HLA-A2.1 transgenic mice were immunized once with transformed attenuated Salmonella . HCV-specific CD8 + T cells were analyzed in vitro as well as in vivo by challenge of mice with recombinant HCV-NS3 vaccinia virus. Results: Salmonella (10 8 colony-forming units; 20 μg plasmid DNA) induced cytotoxic and IFN-γ–producing CD8 + T cells specific for the immunodominant epitope NS3…
Surface Soil Moisture Retrieval Using the L-Band Synthetic Aperture Radar Onboard the Soil Moisture Active–Passive Satellite and Evaluation at Core V…
2017
This paper evaluates the retrieval of soil moisture in the top 5-cm layer at 3-km spatial resolution using L-band dual-copolarized Soil Moisture Active–Passive (SMAP) synthetic aperture radar (SAR) data that mapped the globe every three days from mid-April to early July, 2015. Surface soil moisture retrievals using radar observations have been challenging in the past due to complicating factors of surface roughness and vegetation scattering. Here, physically based forward models of radar scattering for individual vegetation types are inverted using a time-series approach to retrieve soil moisture while correcting for the effects of static roughness and dynamic vegetation. Compared with the …
Clustering-based robust three-dimensional phase unwrapping algorithm
2010
Relatively recent techniques that produce phase volumes have motivated the study of three-dimensional (3D) unwrapping algorithms that inherently incorporate the third dimension into the process. We propose a novel 3D unwrapping algorithm that can be considered to be a generalization of the minimum spanning tree (MST) approach. The technique combines characteristics of some of the most robust existing methods: it uses a quality map to guide the unwrapping process, a region growing mechanism to progressively unwrap the signal, and also cut surfaces to avoid error propagation. The approach has been evaluated in the context of noncontact measurement of dynamic objects, suggesting a better perfo…
The Molecular Analysis of the Light Adaptation Reactions in the Yellow‐green Alga Pleurochloris meiringensis (Xanthophyceae)
1988
The xanthophycean alga Pleurochloris meiringensis was homocontinuously cultured under high light (16 W/m2) and low light (2 W/m2) conditions. In low light cells, the chlorophyll a content and the dry weight on per cell basis is increased, the maximal photosynthetic capacity per chlorophyll is decreased. The content of chlorophyll c, vaucheriaxanthin-ester and heteroxanthin is similar in both cultures, whereas the content of diadinoxanthin and s-carotene is twice as high in high light cultures. High light cells contain more photosystem I and cytochrome f per chlorophyll than low light cells, whereas the QB content is found to be unchanged. Therefore, the ratio reaction center II/reaction cen…