Search results for "T1"
showing 10 items of 587 documents
SARS-CoV-2 vaccine response and rate of breakthrough infection in patients with hematological disorders
2022
Abstract Background The clinical efficacy of SARS-CoV-2 vaccines according to antibody response in immunosuppressed patients such as hematological patients has not yet been established. Patients and methods A prospective multicenter registry-based cohort study conducted from December 2020 to December 2021 by the Spanish transplant and cell therapy group was used to analyze the relationship of antibody response at 3–6 weeks after full vaccination (2 doses) with breakthrough SARS-CoV-2 infection in 1394 patients with hematological disorders. Results At a median follow-up of 165 days after complete immunization, 37 out of 1394 (2.6%) developed breakthrough SARS-CoV-2 infection at median of 77 …
Motivations, engagement and adoption of e-WOM in restaurants.
2022
Resumen El objetivo del presente trabajo fue estudiar el efecto que tienen las motivaciones para consultar y escribir e-WOM sobre el compromiso con el e-WOM y la influencia de dicho compromiso en la adopción del e-WOM consultado. Para analizar estas relaciones, se realizó una investigación empírica aplicada en el contexto de los restaurantes. El ámbito geográfico fue Ecuador, con una muestra de 461 consumidores. Se utilizó un modelo de ecuaciones estructurales y se validó la cadena de relaciones. Los resultados confirmaron las relaciones entre estas variables; además, se presentaron implicaciones académicas interesantes para profundizar en el estudio sobre el compromiso con el e-WOM, así co…
¿Cómo afecta la innovación en la satisfacción y la lealtad hacia el establecimiento minorista?
2021
Resumen En el presente estudio, se examinó el concepto de innovación en el sector minorista y se definieron sus relaciones con otras variables, como la satisfacción y la lealtad, tradicionalmente vinculadas con el establecimiento minorista. Para lograr los objetivos planteados, se delimitó un modelo teórico sustentado en la literatura, que se contrastó mediante un estudio empírico utilizando un cuestionario estructurado ad hoc aplicado a una muestra de 510 clientes de establecimientos de alimentación. El análisis de los datos se desarrolló mediante la técnica de regresión por mínimos cuadrados parciales. Los resultados permiten proponer un conjunto de recomendaciones para la gestión, fundam…
Experimental and numerical investigation on a new FSW based metal to composite joining technique
2018
Abstract In the last decades, different techniques were proposed to join aluminum sheets with composites materials. Each of them has advantages and weak points over the others and new techniques and patents are continuously developed to overcome these difficulties. In this paper an experimental and numerical investigation on a new Friction Stir Welding based approach to mechanically join AA6082-T6 to self-reinforced polypropylene is presented. The aluminum sheet is pre-holed along both the sides of the weld line and a pinless tool generates the heat and pressure needed to prompt back-extrusion of the composite. New experimental fixtures and hole designs were investigated in order to enhance…
Proliferation of aneuploid cells induced by CENP-E depletion is counteracted by the p14ARF tumor suppressor
2018
The spindle assembly checkpoint (SAC) is a cellular surveillance mechanism that ensures the fidelity of chromosomes segregation. Reduced expression of some of its components weakens the SAC and induces chromosome instability and aneuploidy, which are both well-known hallmarks of cancer cells. Centromere protein-E (CENP-E) is a crucial component of the SAC and its function is to facilitate kinetochore microtubule attachment required to achieve and maintain chromosome alignment. The present study investigates the possible role of p14ARF as a controller of aneuploid cells proliferation. We used RNA interference to induce aneuploidy by partial depletion of CENP-E in human primary fibroblasts (I…
A self-inducible heterologous protein expression system in Escherichia coli
2016
AbstractEscherichia coli is an important experimental, medical and industrial cell factory for recombinant protein production. The inducible lac promoter is one of the most commonly used promoters for heterologous protein expression in E. coli. Isopropyl-β-D-thiogalactoside (IPTG) is currently the most efficient molecular inducer for regulating this promoter’s transcriptional activity. However, limitations have been observed in large-scale and microplate production, including toxicity, cost and culture monitoring. Here, we report the novel SILEX (Self-InducibLe Expression) system, which is a convenient, cost-effective alternative that does not require cell density monitoring or IPTG inducti…
Assessment of genetically modified maize Bt11 x MIR162 x 1507 x GA21 and three subcombinations independently of their origin, for food and feed uses …
2018
In this opinion, the GMO Panel assessed the four-event stack maize Btll x MIR162 x 1507 x GA21 and three of its subcombinations, independently of their origin. The GMO Panel previously assessed the four single events and seven of their combinations and did not identify safety concerns. No new data on the single events or the seven subcombinations leading to modification of the original conclusions were identified. Based on the molecular, agronomic, phenotypic and compositional characteristics, the combination of the single events in the four-event stack maize did not give rise to food/feed safety issues. Based on the nutritional assessment of the compositional characteristics of maize Btll …
Evaluating the material resource efficiency of secondary aluminium production: A Monte Carlo-based decision-support tool
2019
The contamination of aluminium streams during the different life cycle stages by alloy mixing and/or accumulation of foreign elements, in combination with the limited melt purification options during remelting, represents an important limiting factor in recycling. Consequently, in secondary aluminium production, primary aluminium is used to dilute the concentration of the residual elements, and alloying elements are added to adjust the composition to the target alloy specifications. However, adding elements, for which their refinement in a subsequent recycling step is problematic, results in permanent down-cycling or ‘quality losses’. Hence, it is crucial to more efficiently control the com…
Análisis para la Implementación del Sistema de Gestión de Calidad y del Sistema de Gestión Ambiental para el Laboratorio de Análisis Instrumental de …
2019
El Laboratorio de Análisis Instrumental ubicado en el 5to piso del Edificio No. 17 de la Escuela Politécnica Nacional brinda servicios de análisis de gases de pozo, biogás, biocidas, solventes, vidrios laminados y templados, entre otros; no obstante, no cuenta con un sistema integrado de Gestión que incluya la ISO 9001:2015 ni ISO 14001:2015, los cuales deberían ser una base fundamental a fin de permitir una dirección basada en la optimización de procesos con una disminución de los impactos ambientales que puede generar cualquier empresa. Con estos antecedentes, el presente trabajo tiene por objetivo desarrollar un mecanismo para implementación de un SIG y que vaya acorde a la normativa ISO…
Process mechanics in Friction Stir Extrusion of magnesium alloys chips through experiments and numerical simulation
2017
Abstract Friction Stir Extrusion (FSE) is a novel process designed to directly recycle machining chips. An experimental campaign was carried out on AZ31 milling chips using variations in extrusion ratio, force and tool rotation rate. The process mechanics were studied and correlated to the material flow, which was elucidated through use of a copper marker. A 3D, Lagrangian, thermo-mechanically coupled dedicated numerical model was set up and validated through temperature measurements. The combination of experimental and numerical results permitted to reconstruct the complex 3D material flow induced by tool rotation and plunge into the extrusion billet chamber.