Search results for "TAU"

GAL4-responsive UAS- tau as a tool for studying the anatomy and development of the Drosophila central nervous system

1997

To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.

Il metodo del confronto: un laboratorio di idee per il recupero della centrale termoelettrica “Tifeo” di Augusta

2018

Il saggio espone un lavoro di ricerca attorno ai temi del “restauro del Moderno” applicato sul recupero della centrale termoelettrica di Augusta, pregevole opera di Giuseppe Samonà. Tale ricerca si è sviluppata nell’ambito del Dottorato di ricerca in Progettazione Architettonica di Palermo grazie ad un protocollo d’intesa firmato cn ENEL. I presupposti metodologici della ricerca , si fondano sulla coincidenza tra restauro e progetto e sulla consapevolezza che l’esperienza del progetto di architettura (ancor più se attuato sul corpo di architetture di qualità già esistenti) sia un fattore di conoscenza decisivo e che solo mettendo il manufatto alla prova del progetto, è possibile acquisire (…

Piano col colore! Mezzo secolo di contraffazioni nei centri storici

2010

The transformation dynamics of the ancient towns are completely changing the urban landscape of the historical countries in Italy in the last fifty years. The most superficial part of material constitutes the image of the architectures and plays a meaningful role in the perception of the historical districts that still characterize the contemporary city, but the aptitude for destroying architectural finishes and deleting superficial patina involves therefore to alter the character of the old towns irreversibly. It had to be obvious it would have been essential to conserve the authentic materials and signs of time that denote the historical authenticity of monuments, but new surfaces of plas…

Streptolysin O-permeabilized granulocytes shed L-selectin concomitantly with ceramide generation via neutral sphingomyelinase

2000

Abstract Cleavage of membrane-associated L-selectin regulates leukocyte rolling on vascular endothelium at sites of inflammation. We report that rapid and massive shedding of L-selectin occurs from granulocytes attacked by the pore-forming bacterial toxin streptolysin O (SLO). Shedding was not induced by an SLO mutant that retained binding capacity but lacked pore-forming activity. Cells permeabilized with SLO exhibited a 1.5-fold increase in the activity of neutral sphingomyelinase, which was accompanied by increased ceramide formation. L-selectin cleavage was inducible by treatment of cells with bacterial sphingomyelinase, and also through exogenous application of a cell-permeable ceramid…

Quelle organisation pour répondre aux changements de société et aux nouvelles générations ? Cinq préconisations

2017

Identity Double-Proton Transfer in (3Z)-3-Hydroxy-1,4-di(quinolin-2-yl)but-3-en-2-one

2003

Although there is a very fast (on the NMR timescale) double-proton transfer in (1Z,3Z)-3-hydroxy-4-quinolin-2-yl-1-quinolin-2(1H)-ylidenbut-3-en-2-one (the product of the condensation of ethyl oxalate with 2-lithiomethylquinoline), it is the only species present in chloroform solution. Comparison of the product of condensation of ethyl oxalate with 2-lithiomethyl derivatives of pyridine (recent studies) and quinoline (present studies) shows that benzoannulation considerably affects the tautomeric equilibrium. The observed changes are not only quantitative but also qualitative. Moreover, contrary to the proton transfer in the pyridine tautomers, this process is fast in the quinoline tautomer…

The Effect of Paddle Stroke Variables Measured by Trainesense SmartPaddle® on the Velocity of the Kayak

2022

(1) Background: This study aimed to compare key variables of paddle stroke measured by a commercial Trainesense SmartPaddle® against the strain-gauge shaft and investigate how these variables are associated with the velocity of the boat among national-level canoe polo players. (2) Methods: This study involved 14 Finnish national-level canoe polo players. The measurement protocol consisted of three different paddling velocities, which were performed in indoor swimming pools. The velocity of the boat was calculated based on the performance time measured with the laser photocell gate. Canoe polo equipment was used in the study and a SmartPaddle sensor was attached to the paddle blade. A strain…

Sesquiterpene Lactones fromCentaurea paui

1994

Abstract A new elemanolide and two new heliangolides as well as (2R,3R)-(+)-3-hydroxy-2-methyl-butyrolactone have been isolated from Centaurea paui. The structures were elucidated by high field NMR techniques and chemical transformation.

GIAO/DFT calculated chemical shifts of tautomeric species. 2-Phenacylpyridines and (Z)-2-(2-hydroxy-2-phenylvinyl)pyridines

2001

1H, 13C and 15N NMR chemical shifts for 28 substituted 2-phenacylpyridines (ketimine forms) and their enolimine tautomers, (Z)-2-(2-hydroxy-2-phenylvinyl)pyridines, were calculated via the GIAO/DFT approach. Among four tested methods at the B3LYP level of theory, the 6–311G, 6–311++G and 6–311G** basis sets gave acceptable result for 13C NMR chemical shifts whereas the 6–311++G** basis set was the minimum needed for reproduction of 15N NMR chemical shifts. Satisfactory reproduction of 13C and 15N NMR chemical shifts for different tautomers revealed that intramolecular hydrogen bonding could be modeled reliably by these calculations when the geometry optimizations were done with the HF/3–21G…

A molecular electron density theory (MEDT) study of the role of halogens (X2= F2, Cl2, Br2and I2) on the aza-Michael-addition reactions

2020

Using the Molecular Electron Density Theory (MEDT), a set of uncatalyzed and X2 molecule-catalyzed (X2 = F2, Cl2, Br2, or I2) aza Michael addition reactions (aza-MARs) between pyrrolidine (PYR) and methyl acrylate (MA) have been studied in the gas phase and in dichloromethane (DCM) medium from the kinetics and molecular mechanism aspects. Analysis of the conceptual DFT reactivity indices indicates that the coordination of the X2 molecules to the oxygen of the MA's carbonyl group increases both the softness and the electrophilic character of MA, thus explaining the catalytic role of the X2 molecules. The nucleophilic attack of the nitrogen of PYR on the MA's β conjugated carbon is the rate-d…